Characterization of blood-derived exosomal proteins after exercise

OBJECTIVE: To assess changes in plasma exosome levels and protein content in mice after long-term exercise. METHODS: We subjected 9-month-old adult C57BL/6J mice to daily treadmill running exercise for 4 weeks prior to the isolation of blood-derived exosomes. Exosomal proteins were identified using...

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Detalles Bibliográficos
Autores principales: Xiang, Hongkai, Chen, Shisheng, Zhou, Junhan, Guo, Junxiu, Zhou, Qingfeng, Zhou, Qishuang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Pre-Clinical Research Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522842/
https://www.ncbi.nlm.nih.gov/pubmed/32972266
http://dx.doi.org/10.1177/0300060520957541
Descripción
Sumario:OBJECTIVE: To assess changes in plasma exosome levels and protein content in mice after long-term exercise. METHODS: We subjected 9-month-old adult C57BL/6J mice to daily treadmill running exercise for 4 weeks prior to the isolation of blood-derived exosomes. Exosomal proteins were identified using mass spectrometry. RESULTS: Extracellular bodies were successfully isolated from mouse blood. Protein levels were altered in blood-derived exosomes after chronic treadmill exercise. Levels of the secretagogue secretogranin 2 were markedly elevated in exercise-induced exosomes. CONCLUSION: Our data suggest that levels of secretogranin 2 were increased in mouse exosomes following chronic treadmill exercise. We conclude that exercise increases exocrine secretion of secretogranin 2.

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