Characterization of blood-derived exosomal proteins after exercise

OBJECTIVE: To assess changes in plasma exosome levels and protein content in mice after long-term exercise. METHODS: We subjected 9-month-old adult C57BL/6J mice to daily treadmill running exercise for 4 weeks prior to the isolation of blood-derived exosomes. Exosomal proteins were identified using...

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Autores principales: Xiang, Hongkai, Chen, Shisheng, Zhou, Junhan, Guo, Junxiu, Zhou, Qingfeng, Zhou, Qishuang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Pre-Clinical Research Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522842/
https://www.ncbi.nlm.nih.gov/pubmed/32972266
http://dx.doi.org/10.1177/0300060520957541
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author Xiang, Hongkai
Chen, Shisheng
Zhou, Junhan
Guo, Junxiu
Zhou, Qingfeng
Zhou, Qishuang
author_facet Xiang, Hongkai
Chen, Shisheng
Zhou, Junhan
Guo, Junxiu
Zhou, Qingfeng
Zhou, Qishuang
author_sort Xiang, Hongkai
collection PubMed
description OBJECTIVE: To assess changes in plasma exosome levels and protein content in mice after long-term exercise. METHODS: We subjected 9-month-old adult C57BL/6J mice to daily treadmill running exercise for 4 weeks prior to the isolation of blood-derived exosomes. Exosomal proteins were identified using mass spectrometry. RESULTS: Extracellular bodies were successfully isolated from mouse blood. Protein levels were altered in blood-derived exosomes after chronic treadmill exercise. Levels of the secretagogue secretogranin 2 were markedly elevated in exercise-induced exosomes. CONCLUSION: Our data suggest that levels of secretogranin 2 were increased in mouse exosomes following chronic treadmill exercise. We conclude that exercise increases exocrine secretion of secretogranin 2.
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spelling pubmed-75228422020-10-06 Characterization of blood-derived exosomal proteins after exercise Xiang, Hongkai Chen, Shisheng Zhou, Junhan Guo, Junxiu Zhou, Qingfeng Zhou, Qishuang J Int Med Res Pre-Clinical Research Report OBJECTIVE: To assess changes in plasma exosome levels and protein content in mice after long-term exercise. METHODS: We subjected 9-month-old adult C57BL/6J mice to daily treadmill running exercise for 4 weeks prior to the isolation of blood-derived exosomes. Exosomal proteins were identified using mass spectrometry. RESULTS: Extracellular bodies were successfully isolated from mouse blood. Protein levels were altered in blood-derived exosomes after chronic treadmill exercise. Levels of the secretagogue secretogranin 2 were markedly elevated in exercise-induced exosomes. CONCLUSION: Our data suggest that levels of secretogranin 2 were increased in mouse exosomes following chronic treadmill exercise. We conclude that exercise increases exocrine secretion of secretogranin 2. SAGE Publications 2020-09-24 /pmc/articles/PMC7522842/ /pubmed/32972266 http://dx.doi.org/10.1177/0300060520957541 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Pre-Clinical Research Report
Xiang, Hongkai
Chen, Shisheng
Zhou, Junhan
Guo, Junxiu
Zhou, Qingfeng
Zhou, Qishuang
Characterization of blood-derived exosomal proteins after exercise
title Characterization of blood-derived exosomal proteins after exercise
title_full Characterization of blood-derived exosomal proteins after exercise
title_fullStr Characterization of blood-derived exosomal proteins after exercise
title_full_unstemmed Characterization of blood-derived exosomal proteins after exercise
title_short Characterization of blood-derived exosomal proteins after exercise
title_sort characterization of blood-derived exosomal proteins after exercise
topic Pre-Clinical Research Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522842/
https://www.ncbi.nlm.nih.gov/pubmed/32972266
http://dx.doi.org/10.1177/0300060520957541
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