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Expressions of miR-155 and miR-181 and predictions of their structures and targets in pigs (Sus scrofa)
BACKGROUND AND AIM: MicroRNAs (miRNAs) are responsible for gene expression control at the post-transcription level in many species. Several miRNAs are required in the regulation of immune responses, such as B-cell differentiation, T-cell receptor signaling pathway, CD4(+) T cell selection, and so on...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522940/ https://www.ncbi.nlm.nih.gov/pubmed/33061243 http://dx.doi.org/10.14202/vetworld.2020.1667-1673 |
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author | Ninsuwon, Jirapat Waiyamitra, Pitchaporn Roongsitthichai, Atthaporn Surachetpong, Win |
author_facet | Ninsuwon, Jirapat Waiyamitra, Pitchaporn Roongsitthichai, Atthaporn Surachetpong, Win |
author_sort | Ninsuwon, Jirapat |
collection | PubMed |
description | BACKGROUND AND AIM: MicroRNAs (miRNAs) are responsible for gene expression control at the post-transcription level in many species. Several miRNAs are required in the regulation of immune responses, such as B-cell differentiation, T-cell receptor signaling pathway, CD4(+) T cell selection, and so on. Studies on miRNAs have been extensively conducted in humans and mice; however, reports relevant to miRNAs, especially miR-155 and miR-181, in pigs are limited. Consequently, the present study aimed to investigate the structures, target genes, and expressions of miR-155 and miR-181 in various porcine cells and tissues. MATERIALS AND METHODS: Five healthy male pigs from a porcine reproductive and respiratory syndrome virus-negative farm were studied. Before slaughter, blood samples were collected for peripheral blood mononuclear cell isolation. After slaughter, samples of spleen, lymph nodes, and forelimb muscles were collected. Both miR-155 and miR-181 were investigated for their structures with RNAfold web server, for their target genes from three online web servers, and for their expressions using polymerase chain reaction (PCR). RESULTS: The structures of miR-155 and miR-181 contained hairpins with free energies of −35.27 and −35.29 kcal/mole, respectively. Target gene prediction revealed that miR-155 had perfect complementarity with Socs1 and Mapk3k14, while miR-181 had perfect complementarity with Ddx3x, Nfat5, Foxp1, and Mpp5. PCR showed that both miRNAs were detectable from all investigated cells and tissues. Moreover, the highest expression of both miRNAs was found from the lymph node of the pigs. CONCLUSION: Both miR-155 and miR-181 might be involved with the regulation of porcine immune functions as both miRNAs were detected in several cells and tissues of the pigs. In addition, they had very high complementarities with the seed regions of several immune-related genes. |
format | Online Article Text |
id | pubmed-7522940 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-75229402020-10-14 Expressions of miR-155 and miR-181 and predictions of their structures and targets in pigs (Sus scrofa) Ninsuwon, Jirapat Waiyamitra, Pitchaporn Roongsitthichai, Atthaporn Surachetpong, Win Vet World Research Article BACKGROUND AND AIM: MicroRNAs (miRNAs) are responsible for gene expression control at the post-transcription level in many species. Several miRNAs are required in the regulation of immune responses, such as B-cell differentiation, T-cell receptor signaling pathway, CD4(+) T cell selection, and so on. Studies on miRNAs have been extensively conducted in humans and mice; however, reports relevant to miRNAs, especially miR-155 and miR-181, in pigs are limited. Consequently, the present study aimed to investigate the structures, target genes, and expressions of miR-155 and miR-181 in various porcine cells and tissues. MATERIALS AND METHODS: Five healthy male pigs from a porcine reproductive and respiratory syndrome virus-negative farm were studied. Before slaughter, blood samples were collected for peripheral blood mononuclear cell isolation. After slaughter, samples of spleen, lymph nodes, and forelimb muscles were collected. Both miR-155 and miR-181 were investigated for their structures with RNAfold web server, for their target genes from three online web servers, and for their expressions using polymerase chain reaction (PCR). RESULTS: The structures of miR-155 and miR-181 contained hairpins with free energies of −35.27 and −35.29 kcal/mole, respectively. Target gene prediction revealed that miR-155 had perfect complementarity with Socs1 and Mapk3k14, while miR-181 had perfect complementarity with Ddx3x, Nfat5, Foxp1, and Mpp5. PCR showed that both miRNAs were detectable from all investigated cells and tissues. Moreover, the highest expression of both miRNAs was found from the lymph node of the pigs. CONCLUSION: Both miR-155 and miR-181 might be involved with the regulation of porcine immune functions as both miRNAs were detected in several cells and tissues of the pigs. In addition, they had very high complementarities with the seed regions of several immune-related genes. Veterinary World 2020-08 2020-08-21 /pmc/articles/PMC7522940/ /pubmed/33061243 http://dx.doi.org/10.14202/vetworld.2020.1667-1673 Text en Copyright: © Ninsuwon, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Ninsuwon, Jirapat Waiyamitra, Pitchaporn Roongsitthichai, Atthaporn Surachetpong, Win Expressions of miR-155 and miR-181 and predictions of their structures and targets in pigs (Sus scrofa) |
title | Expressions of miR-155 and miR-181 and predictions of their structures and targets in pigs (Sus scrofa) |
title_full | Expressions of miR-155 and miR-181 and predictions of their structures and targets in pigs (Sus scrofa) |
title_fullStr | Expressions of miR-155 and miR-181 and predictions of their structures and targets in pigs (Sus scrofa) |
title_full_unstemmed | Expressions of miR-155 and miR-181 and predictions of their structures and targets in pigs (Sus scrofa) |
title_short | Expressions of miR-155 and miR-181 and predictions of their structures and targets in pigs (Sus scrofa) |
title_sort | expressions of mir-155 and mir-181 and predictions of their structures and targets in pigs (sus scrofa) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522940/ https://www.ncbi.nlm.nih.gov/pubmed/33061243 http://dx.doi.org/10.14202/vetworld.2020.1667-1673 |
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