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Histological classification and expression of markers of canine mast cell tumors

BACKGROUND AND AIM: Mast cell tumors (MCTs) are malignant neoplasms that are common in dogs. Their biological behavior is variable and unpredictable. The aim of the present study was to analyze the histological classification and expression of markers of canine MCTs. MATERIALS AND METHODS: Thirty sa...

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Autores principales: Cruz, V. S., Borges, J. C. A., Nepomuceno, L. L., Gonçalves, P. A. M., Prado, Y. C. L., Bianchi, C., Fioravanti, M. C. S., Araújo, E. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522949/
https://www.ncbi.nlm.nih.gov/pubmed/33061237
http://dx.doi.org/10.14202/vetworld.2020.1627-1634
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author Cruz, V. S.
Borges, J. C. A.
Nepomuceno, L. L.
Gonçalves, P. A. M.
Prado, Y. C. L.
Bianchi, C.
Fioravanti, M. C. S.
Araújo, E. G.
author_facet Cruz, V. S.
Borges, J. C. A.
Nepomuceno, L. L.
Gonçalves, P. A. M.
Prado, Y. C. L.
Bianchi, C.
Fioravanti, M. C. S.
Araújo, E. G.
author_sort Cruz, V. S.
collection PubMed
description BACKGROUND AND AIM: Mast cell tumors (MCTs) are malignant neoplasms that are common in dogs. Their biological behavior is variable and unpredictable. The aim of the present study was to analyze the histological classification and expression of markers of canine MCTs. MATERIALS AND METHODS: Thirty samples of canine MCTs were graded according to the histological classification methods of Patnaik and those of Kiupel. The expression of phosphoprotein 53 (p53) and c-kit proteins was quantified by immunohistochemistry using image processing software, ImageJ - a public domain computer program, developed at the National Institutes of Health. RESULTS: It was possible to determine the grade of 100% of the samples. According to Patnaik’s classification, 20.00% of the samples were Grade 1, 43.30% were Grade 2, and 36.70% were Grade 3. According to Kiupel’s classification, 56.67% of the samples were of high intensity and 43.33% were of low intensity. Grade 1 tumors had the highest expression of p53 and c-kit, and Grade 2 had the lowest expression. The results showed that it is necessary to perform both histological grading methods. The classification into high and low intensity may provide more consistent results than the three-level grading system. However, a smaller number of categories, although it facilitates the classification, may not be sufficient for the prognosis. CONCLUSION: Quantitative evaluation of p-53 and c-kit expression is a useful tool to increase the accuracy of the analysis and to aid in choosing the treatment method for canine MCTs. Histological grading should be combined with other diagnostic methods.
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spelling pubmed-75229492020-10-14 Histological classification and expression of markers of canine mast cell tumors Cruz, V. S. Borges, J. C. A. Nepomuceno, L. L. Gonçalves, P. A. M. Prado, Y. C. L. Bianchi, C. Fioravanti, M. C. S. Araújo, E. G. Vet World Research Article BACKGROUND AND AIM: Mast cell tumors (MCTs) are malignant neoplasms that are common in dogs. Their biological behavior is variable and unpredictable. The aim of the present study was to analyze the histological classification and expression of markers of canine MCTs. MATERIALS AND METHODS: Thirty samples of canine MCTs were graded according to the histological classification methods of Patnaik and those of Kiupel. The expression of phosphoprotein 53 (p53) and c-kit proteins was quantified by immunohistochemistry using image processing software, ImageJ - a public domain computer program, developed at the National Institutes of Health. RESULTS: It was possible to determine the grade of 100% of the samples. According to Patnaik’s classification, 20.00% of the samples were Grade 1, 43.30% were Grade 2, and 36.70% were Grade 3. According to Kiupel’s classification, 56.67% of the samples were of high intensity and 43.33% were of low intensity. Grade 1 tumors had the highest expression of p53 and c-kit, and Grade 2 had the lowest expression. The results showed that it is necessary to perform both histological grading methods. The classification into high and low intensity may provide more consistent results than the three-level grading system. However, a smaller number of categories, although it facilitates the classification, may not be sufficient for the prognosis. CONCLUSION: Quantitative evaluation of p-53 and c-kit expression is a useful tool to increase the accuracy of the analysis and to aid in choosing the treatment method for canine MCTs. Histological grading should be combined with other diagnostic methods. Veterinary World 2020-08 2020-08-18 /pmc/articles/PMC7522949/ /pubmed/33061237 http://dx.doi.org/10.14202/vetworld.2020.1627-1634 Text en Copyright: © Cruz, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Cruz, V. S.
Borges, J. C. A.
Nepomuceno, L. L.
Gonçalves, P. A. M.
Prado, Y. C. L.
Bianchi, C.
Fioravanti, M. C. S.
Araújo, E. G.
Histological classification and expression of markers of canine mast cell tumors
title Histological classification and expression of markers of canine mast cell tumors
title_full Histological classification and expression of markers of canine mast cell tumors
title_fullStr Histological classification and expression of markers of canine mast cell tumors
title_full_unstemmed Histological classification and expression of markers of canine mast cell tumors
title_short Histological classification and expression of markers of canine mast cell tumors
title_sort histological classification and expression of markers of canine mast cell tumors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522949/
https://www.ncbi.nlm.nih.gov/pubmed/33061237
http://dx.doi.org/10.14202/vetworld.2020.1627-1634
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