Neutralization Assay with SARS-CoV-1 and SARS-CoV-2 Spike Pseudotyped Murine Leukemia Virions

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Antibody neutralization is an important prognostic factor in many viral diseases. To easily and rapidly measure titers of neutralizing antibodies in serum or plasma, we developed pseudovirion particles composed of the spike glycoprotein of SARS-CoV-2 incorporated onto murine leukemia virus capsids a...

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Detalles Bibliográficos
Autores principales: Zheng, Yue, Larragoite, Erin T., Lama, Juan, Cisneros, Isabel, Delgado, Julio C., Slev, Patricia, Rychert, Jenna, Innis, Emily A., Williams, Elizabeth S.C.P., Coiras, Mayte, Rondina, Matthew T., Spivak, Adam M., Planelles, Vicente
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523104/
https://www.ncbi.nlm.nih.gov/pubmed/32995778
http://dx.doi.org/10.1101/2020.07.17.207563
Descripción
Sumario:Antibody neutralization is an important prognostic factor in many viral diseases. To easily and rapidly measure titers of neutralizing antibodies in serum or plasma, we developed pseudovirion particles composed of the spike glycoprotein of SARS-CoV-2 incorporated onto murine leukemia virus capsids and a modified minimal MLV genome encoding firefly luciferase. These pseudovirions provide a practical means of assessing immune responses under laboratory conditions consistent with biocontainment level 2.

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