Neutralization Assay with SARS-CoV-1 and SARS-CoV-2 Spike Pseudotyped Murine Leukemia Virions

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Antibody neutralization is an important prognostic factor in many viral diseases. To easily and rapidly measure titers of neutralizing antibodies in serum or plasma, we developed pseudovirion particles composed of the spike glycoprotein of SARS-CoV-2 incorporated onto murine leukemia virus capsids a...

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Autores principales: Zheng, Yue, Larragoite, Erin T., Lama, Juan, Cisneros, Isabel, Delgado, Julio C., Slev, Patricia, Rychert, Jenna, Innis, Emily A., Williams, Elizabeth S.C.P., Coiras, Mayte, Rondina, Matthew T., Spivak, Adam M., Planelles, Vicente
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523104/
https://www.ncbi.nlm.nih.gov/pubmed/32995778
http://dx.doi.org/10.1101/2020.07.17.207563
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author Zheng, Yue
Larragoite, Erin T.
Lama, Juan
Cisneros, Isabel
Delgado, Julio C.
Slev, Patricia
Rychert, Jenna
Innis, Emily A.
Williams, Elizabeth S.C.P.
Coiras, Mayte
Rondina, Matthew T.
Spivak, Adam M.
Planelles, Vicente
author_facet Zheng, Yue
Larragoite, Erin T.
Lama, Juan
Cisneros, Isabel
Delgado, Julio C.
Slev, Patricia
Rychert, Jenna
Innis, Emily A.
Williams, Elizabeth S.C.P.
Coiras, Mayte
Rondina, Matthew T.
Spivak, Adam M.
Planelles, Vicente
author_sort Zheng, Yue
collection PubMed
description Antibody neutralization is an important prognostic factor in many viral diseases. To easily and rapidly measure titers of neutralizing antibodies in serum or plasma, we developed pseudovirion particles composed of the spike glycoprotein of SARS-CoV-2 incorporated onto murine leukemia virus capsids and a modified minimal MLV genome encoding firefly luciferase. These pseudovirions provide a practical means of assessing immune responses under laboratory conditions consistent with biocontainment level 2.
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spelling pubmed-75231042020-09-30 Neutralization Assay with SARS-CoV-1 and SARS-CoV-2 Spike Pseudotyped Murine Leukemia Virions Zheng, Yue Larragoite, Erin T. Lama, Juan Cisneros, Isabel Delgado, Julio C. Slev, Patricia Rychert, Jenna Innis, Emily A. Williams, Elizabeth S.C.P. Coiras, Mayte Rondina, Matthew T. Spivak, Adam M. Planelles, Vicente bioRxiv Article Antibody neutralization is an important prognostic factor in many viral diseases. To easily and rapidly measure titers of neutralizing antibodies in serum or plasma, we developed pseudovirion particles composed of the spike glycoprotein of SARS-CoV-2 incorporated onto murine leukemia virus capsids and a modified minimal MLV genome encoding firefly luciferase. These pseudovirions provide a practical means of assessing immune responses under laboratory conditions consistent with biocontainment level 2. Cold Spring Harbor Laboratory 2020-09-21 /pmc/articles/PMC7523104/ /pubmed/32995778 http://dx.doi.org/10.1101/2020.07.17.207563 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/It is made available under a CC-BY-NC-ND 4.0 International license (http://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Article
Zheng, Yue
Larragoite, Erin T.
Lama, Juan
Cisneros, Isabel
Delgado, Julio C.
Slev, Patricia
Rychert, Jenna
Innis, Emily A.
Williams, Elizabeth S.C.P.
Coiras, Mayte
Rondina, Matthew T.
Spivak, Adam M.
Planelles, Vicente
Neutralization Assay with SARS-CoV-1 and SARS-CoV-2 Spike Pseudotyped Murine Leukemia Virions
title Neutralization Assay with SARS-CoV-1 and SARS-CoV-2 Spike Pseudotyped Murine Leukemia Virions
title_full Neutralization Assay with SARS-CoV-1 and SARS-CoV-2 Spike Pseudotyped Murine Leukemia Virions
title_fullStr Neutralization Assay with SARS-CoV-1 and SARS-CoV-2 Spike Pseudotyped Murine Leukemia Virions
title_full_unstemmed Neutralization Assay with SARS-CoV-1 and SARS-CoV-2 Spike Pseudotyped Murine Leukemia Virions
title_short Neutralization Assay with SARS-CoV-1 and SARS-CoV-2 Spike Pseudotyped Murine Leukemia Virions
title_sort neutralization assay with sars-cov-1 and sars-cov-2 spike pseudotyped murine leukemia virions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523104/
https://www.ncbi.nlm.nih.gov/pubmed/32995778
http://dx.doi.org/10.1101/2020.07.17.207563
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