The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device

[Image: see text] There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the...

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Autores principales: Baker, Alexander N., Richards, Sarah-Jane, Guy, Collette S., Congdon, Thomas R., Hasan, Muhammad, Zwetsloot, Alexander J., Gallo, Angelo, Lewandowski, Józef R., Stansfeld, Phillip J., Straube, Anne, Walker, Marc, Chessa, Simona, Pergolizzi, Giulia, Dedola, Simone, Field, Robert A., Gibson, Matthew I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523238/
https://www.ncbi.nlm.nih.gov/pubmed/33269329
http://dx.doi.org/10.1021/acscentsci.0c00855
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author Baker, Alexander N.
Richards, Sarah-Jane
Guy, Collette S.
Congdon, Thomas R.
Hasan, Muhammad
Zwetsloot, Alexander J.
Gallo, Angelo
Lewandowski, Józef R.
Stansfeld, Phillip J.
Straube, Anne
Walker, Marc
Chessa, Simona
Pergolizzi, Giulia
Dedola, Simone
Field, Robert A.
Gibson, Matthew I.
author_facet Baker, Alexander N.
Richards, Sarah-Jane
Guy, Collette S.
Congdon, Thomas R.
Hasan, Muhammad
Zwetsloot, Alexander J.
Gallo, Angelo
Lewandowski, Józef R.
Stansfeld, Phillip J.
Straube, Anne
Walker, Marc
Chessa, Simona
Pergolizzi, Giulia
Dedola, Simone
Field, Robert A.
Gibson, Matthew I.
author_sort Baker, Alexander N.
collection PubMed
description [Image: see text] There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.
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spelling pubmed-75232382020-09-30 The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device Baker, Alexander N. Richards, Sarah-Jane Guy, Collette S. Congdon, Thomas R. Hasan, Muhammad Zwetsloot, Alexander J. Gallo, Angelo Lewandowski, Józef R. Stansfeld, Phillip J. Straube, Anne Walker, Marc Chessa, Simona Pergolizzi, Giulia Dedola, Simone Field, Robert A. Gibson, Matthew I. ACS Cent Sci [Image: see text] There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform. American Chemical Society 2020-09-23 2020-11-25 /pmc/articles/PMC7523238/ /pubmed/33269329 http://dx.doi.org/10.1021/acscentsci.0c00855 Text en This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Baker, Alexander N.
Richards, Sarah-Jane
Guy, Collette S.
Congdon, Thomas R.
Hasan, Muhammad
Zwetsloot, Alexander J.
Gallo, Angelo
Lewandowski, Józef R.
Stansfeld, Phillip J.
Straube, Anne
Walker, Marc
Chessa, Simona
Pergolizzi, Giulia
Dedola, Simone
Field, Robert A.
Gibson, Matthew I.
The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device
title The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device
title_full The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device
title_fullStr The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device
title_full_unstemmed The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device
title_short The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device
title_sort sars-cov-2 spike protein binds sialic acids and enables rapid detection in a lateral flow point of care diagnostic device
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523238/
https://www.ncbi.nlm.nih.gov/pubmed/33269329
http://dx.doi.org/10.1021/acscentsci.0c00855
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