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Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation

BACKGROUND: Quantitative polymerase chain reaction (qPCR) is the technique of choice for quantifying gene expression. While the technique itself is well established, approaches for the analysis of qPCR data continue to improve. RESULTS: Here we expand on the common base method to develop procedures...

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Detalles Bibliográficos
Autores principales: Ganger, Michael T., Dietz, Geoffrey D., Headley, Patrick, Ewing, Sarah J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523404/
https://www.ncbi.nlm.nih.gov/pubmed/32993490
http://dx.doi.org/10.1186/s12859-020-03696-y