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First hybrid complete genome of Aeromonas veronii reveals chromosome-mediated novel structural variant mcr-3.30 from a human clinical sample

Recent findings demonstrate the origin of the plasmid-mediated colistin resistance gene mcr-3 from aeromonads. The present study aimed to screen for plasmid-mediated colistin resistance among 30 clinical multidrug-resistant (MDR) Aeromonas spp. PCR was used to screen for the presence of mcr-1, mcr-2...

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Autores principales: Ragupathi, Naveen Kumar Devanga, Sethuvel, Dhiviya Prabaa Muthuirulandi, Anandan, Shalini, Murugan, Dhivya, Asokan, Kalaiarasi, Neethi Mohan, Ramya Gajaraj, Vasudevan, Karthick, D, Thirumal Kumar, C, George Priya Doss, Veeraraghavan, Balaji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523623/
https://www.ncbi.nlm.nih.gov/pubmed/33005867
http://dx.doi.org/10.1099/acmi.0.000103
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author Ragupathi, Naveen Kumar Devanga
Sethuvel, Dhiviya Prabaa Muthuirulandi
Anandan, Shalini
Murugan, Dhivya
Asokan, Kalaiarasi
Neethi Mohan, Ramya Gajaraj
Vasudevan, Karthick
D, Thirumal Kumar
C, George Priya Doss
Veeraraghavan, Balaji
author_facet Ragupathi, Naveen Kumar Devanga
Sethuvel, Dhiviya Prabaa Muthuirulandi
Anandan, Shalini
Murugan, Dhivya
Asokan, Kalaiarasi
Neethi Mohan, Ramya Gajaraj
Vasudevan, Karthick
D, Thirumal Kumar
C, George Priya Doss
Veeraraghavan, Balaji
author_sort Ragupathi, Naveen Kumar Devanga
collection PubMed
description Recent findings demonstrate the origin of the plasmid-mediated colistin resistance gene mcr-3 from aeromonads. The present study aimed to screen for plasmid-mediated colistin resistance among 30 clinical multidrug-resistant (MDR) Aeromonas spp. PCR was used to screen for the presence of mcr-1, mcr-2, mcr-3 and mcr-4, which revealed mcr-3 in a colistin-susceptible isolate (FC951). All other isolates were negative for mcr. Sequencing of FC951 revealed that the mcr-3 (mcr-3.30) identified was different from previously reported variants and had 95.62 and 95.28 % nucleotide similarity with mcr-3.3 and mcr-3.10. Hybrid assembly using IonTorrent and MinION reads revealed structural genetic information for mcr-3.30 with an insertion of ISAs18 within the gene. Due to this, mcr-3.30 was non-expressive, which makes FC951 susceptible to colistin. Further, in silico sequence and protein structural analysis confirmed the new variant. To the best of our knowledge, this is the first report on a novel mcr-3 variant from India. The significant role of mcr-like genes in different Aeromonas species remains unknown and requires additional investigation to obtains insights into the mechanism of colistin resistance.
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spelling pubmed-75236232020-09-30 First hybrid complete genome of Aeromonas veronii reveals chromosome-mediated novel structural variant mcr-3.30 from a human clinical sample Ragupathi, Naveen Kumar Devanga Sethuvel, Dhiviya Prabaa Muthuirulandi Anandan, Shalini Murugan, Dhivya Asokan, Kalaiarasi Neethi Mohan, Ramya Gajaraj Vasudevan, Karthick D, Thirumal Kumar C, George Priya Doss Veeraraghavan, Balaji Access Microbiol Research Article Recent findings demonstrate the origin of the plasmid-mediated colistin resistance gene mcr-3 from aeromonads. The present study aimed to screen for plasmid-mediated colistin resistance among 30 clinical multidrug-resistant (MDR) Aeromonas spp. PCR was used to screen for the presence of mcr-1, mcr-2, mcr-3 and mcr-4, which revealed mcr-3 in a colistin-susceptible isolate (FC951). All other isolates were negative for mcr. Sequencing of FC951 revealed that the mcr-3 (mcr-3.30) identified was different from previously reported variants and had 95.62 and 95.28 % nucleotide similarity with mcr-3.3 and mcr-3.10. Hybrid assembly using IonTorrent and MinION reads revealed structural genetic information for mcr-3.30 with an insertion of ISAs18 within the gene. Due to this, mcr-3.30 was non-expressive, which makes FC951 susceptible to colistin. Further, in silico sequence and protein structural analysis confirmed the new variant. To the best of our knowledge, this is the first report on a novel mcr-3 variant from India. The significant role of mcr-like genes in different Aeromonas species remains unknown and requires additional investigation to obtains insights into the mechanism of colistin resistance. Microbiology Society 2020-02-17 /pmc/articles/PMC7523623/ /pubmed/33005867 http://dx.doi.org/10.1099/acmi.0.000103 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons.
spellingShingle Research Article
Ragupathi, Naveen Kumar Devanga
Sethuvel, Dhiviya Prabaa Muthuirulandi
Anandan, Shalini
Murugan, Dhivya
Asokan, Kalaiarasi
Neethi Mohan, Ramya Gajaraj
Vasudevan, Karthick
D, Thirumal Kumar
C, George Priya Doss
Veeraraghavan, Balaji
First hybrid complete genome of Aeromonas veronii reveals chromosome-mediated novel structural variant mcr-3.30 from a human clinical sample
title First hybrid complete genome of Aeromonas veronii reveals chromosome-mediated novel structural variant mcr-3.30 from a human clinical sample
title_full First hybrid complete genome of Aeromonas veronii reveals chromosome-mediated novel structural variant mcr-3.30 from a human clinical sample
title_fullStr First hybrid complete genome of Aeromonas veronii reveals chromosome-mediated novel structural variant mcr-3.30 from a human clinical sample
title_full_unstemmed First hybrid complete genome of Aeromonas veronii reveals chromosome-mediated novel structural variant mcr-3.30 from a human clinical sample
title_short First hybrid complete genome of Aeromonas veronii reveals chromosome-mediated novel structural variant mcr-3.30 from a human clinical sample
title_sort first hybrid complete genome of aeromonas veronii reveals chromosome-mediated novel structural variant mcr-3.30 from a human clinical sample
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523623/
https://www.ncbi.nlm.nih.gov/pubmed/33005867
http://dx.doi.org/10.1099/acmi.0.000103
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