Molecular characterization of the first reported Aichivirus A in Australia

A novel real-time reverse transcription polymerase chain reaction (RT-rPCR) assay was developed to detect Aichivirus A (AiV-A) based on four complete genomes. The assay successfully detected AiV-A in a sample from a patient with acute gastroenteritis in January 2008. Screening of 756 samples submitt...

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Detalles Bibliográficos
Autores principales: Northill, Judith A., Simmons, Russell J., Genge, Doris, Moore, Frederick A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523624/
https://www.ncbi.nlm.nih.gov/pubmed/33005865
http://dx.doi.org/10.1099/acmi.0.000099
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author Northill, Judith A.
Simmons, Russell J.
Genge, Doris
Moore, Frederick A.
author_facet Northill, Judith A.
Simmons, Russell J.
Genge, Doris
Moore, Frederick A.
author_sort Northill, Judith A.
collection PubMed
description A novel real-time reverse transcription polymerase chain reaction (RT-rPCR) assay was developed to detect Aichivirus A (AiV-A) based on four complete genomes. The assay successfully detected AiV-A in a sample from a patient with acute gastroenteritis in January 2008. Screening of 756 samples submitted for norovirus testing during May 2008 detected a further 23 AiV-A-positive samples from 18 individual patients. Genotyping using novel primers targeting the 3C–3D junction region identified AiV-A genotype B. Further sequencing of the VP1 region supported the 3C–3D result. All three assays proved useful to support foodborne outbreak investigations. This is the first report of AiV-A detection in Australia.
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spelling pubmed-75236242020-09-30 Molecular characterization of the first reported Aichivirus A in Australia Northill, Judith A. Simmons, Russell J. Genge, Doris Moore, Frederick A. Access Microbiol Research Article A novel real-time reverse transcription polymerase chain reaction (RT-rPCR) assay was developed to detect Aichivirus A (AiV-A) based on four complete genomes. The assay successfully detected AiV-A in a sample from a patient with acute gastroenteritis in January 2008. Screening of 756 samples submitted for norovirus testing during May 2008 detected a further 23 AiV-A-positive samples from 18 individual patients. Genotyping using novel primers targeting the 3C–3D junction region identified AiV-A genotype B. Further sequencing of the VP1 region supported the 3C–3D result. All three assays proved useful to support foodborne outbreak investigations. This is the first report of AiV-A detection in Australia. Microbiology Society 2020-02-11 /pmc/articles/PMC7523624/ /pubmed/33005865 http://dx.doi.org/10.1099/acmi.0.000099 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License.
spellingShingle Research Article
Northill, Judith A.
Simmons, Russell J.
Genge, Doris
Moore, Frederick A.
Molecular characterization of the first reported Aichivirus A in Australia
title Molecular characterization of the first reported Aichivirus A in Australia
title_full Molecular characterization of the first reported Aichivirus A in Australia
title_fullStr Molecular characterization of the first reported Aichivirus A in Australia
title_full_unstemmed Molecular characterization of the first reported Aichivirus A in Australia
title_short Molecular characterization of the first reported Aichivirus A in Australia
title_sort molecular characterization of the first reported aichivirus a in australia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523624/
https://www.ncbi.nlm.nih.gov/pubmed/33005865
http://dx.doi.org/10.1099/acmi.0.000099
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