AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level

By including oligonucleotide-labeled antibodies into high-throughput single-cell RNA-sequencing protocols, combined transcript and protein expression data can be acquired on the single-cell level. Here, we describe a protocol for the combined analysis of over 40 proteins and 400 genes on over 10(4)...

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Autores principales: Erickson, Jami R., Mair, Florian, Bugos, Grace, Martin, Jody, Tyznik, Aaron J., Nakamoto, Margaret, Mortimer, Stefanie, Prlic, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523635/
https://www.ncbi.nlm.nih.gov/pubmed/33000001
http://dx.doi.org/10.1016/j.xpro.2020.100092
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author Erickson, Jami R.
Mair, Florian
Bugos, Grace
Martin, Jody
Tyznik, Aaron J.
Nakamoto, Margaret
Mortimer, Stefanie
Prlic, Martin
author_facet Erickson, Jami R.
Mair, Florian
Bugos, Grace
Martin, Jody
Tyznik, Aaron J.
Nakamoto, Margaret
Mortimer, Stefanie
Prlic, Martin
author_sort Erickson, Jami R.
collection PubMed
description By including oligonucleotide-labeled antibodies into high-throughput single-cell RNA-sequencing protocols, combined transcript and protein expression data can be acquired on the single-cell level. Here, we describe a protocol for the combined analysis of over 40 proteins and 400 genes on over 10(4) cells using the nano-well based Rhapsody platform. We also include a workflow for sample multiplexing, which uniquely identifies the initial source of cells (such as tissue type or donor) in the downstream analysis after upstream pooling. For complete information on the use and execution of this protocol, please refer to Mair et al. (2020).
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spelling pubmed-75236352020-09-29 AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level Erickson, Jami R. Mair, Florian Bugos, Grace Martin, Jody Tyznik, Aaron J. Nakamoto, Margaret Mortimer, Stefanie Prlic, Martin STAR Protoc Protocol By including oligonucleotide-labeled antibodies into high-throughput single-cell RNA-sequencing protocols, combined transcript and protein expression data can be acquired on the single-cell level. Here, we describe a protocol for the combined analysis of over 40 proteins and 400 genes on over 10(4) cells using the nano-well based Rhapsody platform. We also include a workflow for sample multiplexing, which uniquely identifies the initial source of cells (such as tissue type or donor) in the downstream analysis after upstream pooling. For complete information on the use and execution of this protocol, please refer to Mair et al. (2020). Elsevier 2020-08-25 /pmc/articles/PMC7523635/ /pubmed/33000001 http://dx.doi.org/10.1016/j.xpro.2020.100092 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Erickson, Jami R.
Mair, Florian
Bugos, Grace
Martin, Jody
Tyznik, Aaron J.
Nakamoto, Margaret
Mortimer, Stefanie
Prlic, Martin
AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level
title AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level
title_full AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level
title_fullStr AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level
title_full_unstemmed AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level
title_short AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level
title_sort abseq protocol using the nano-well cartridge-based rhapsody platform to generate protein and transcript expression data on the single-cell level
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523635/
https://www.ncbi.nlm.nih.gov/pubmed/33000001
http://dx.doi.org/10.1016/j.xpro.2020.100092
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