An optimized step‐by‐step protocol for isolation of nucleus pulposus, annulus fibrosus, and end plate cells from the mouse intervertebral discs and subsequent preparation of high‐quality intact total RNA

Intervertebral disc degeneration is the most significant, and least understood, cause of chronic back pain, affecting almost one in seven individuals at some point of time. Each intervertebral disc has three components; central nucleus pulposus (NP), concentric layers of annulus fibrosus (AF), and a...

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Detalles Bibliográficos
Autores principales: Piprode, Vikrant, Mohanty, Sarthak, Bonavita, Raffaella, Loh, Sarah, Anbazhagan, Rajakumar, Saini, Chandan, Pinelli, Robert, Pricop, Paul, Dahia, Chitra L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2020
Materias:
Protocols, Methods, and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7524240/
https://www.ncbi.nlm.nih.gov/pubmed/33015579
http://dx.doi.org/10.1002/jsp2.1108

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