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Changes in the fecal bacterial microbiota associated with disease severity in alcoholic hepatitis patients

BACKGROUND AND AIMS: Alcoholic hepatitis is the most severe form of alcohol-related liver disease. While the gut microbiome is known to play a role in disease development and progression, less is known about specific compositional changes of the gut bacterial microbiome associated with disease sever...

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Autores principales: Lang, Sonja, Fairfied, Bradley, Gao, Bei, Duan, Yi, Zhang, Xinlian, Fouts, Derrick E., Schnabl, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7524371/
https://www.ncbi.nlm.nih.gov/pubmed/32684075
http://dx.doi.org/10.1080/19490976.2020.1785251
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author Lang, Sonja
Fairfied, Bradley
Gao, Bei
Duan, Yi
Zhang, Xinlian
Fouts, Derrick E.
Schnabl, Bernd
author_facet Lang, Sonja
Fairfied, Bradley
Gao, Bei
Duan, Yi
Zhang, Xinlian
Fouts, Derrick E.
Schnabl, Bernd
author_sort Lang, Sonja
collection PubMed
description BACKGROUND AND AIMS: Alcoholic hepatitis is the most severe form of alcohol-related liver disease. While the gut microbiome is known to play a role in disease development and progression, less is known about specific compositional changes of the gut bacterial microbiome associated with disease severity. Therefore, the aim of our study was to correlate gut microbiota features with disease severity in alcoholic hepatitis patients. METHODS: We used 16S rRNA gene sequencing on fecal samples from 74 alcoholic hepatitis patients, which were enrolled at 9 centers in Europe, the United States, and Mexico in a multi-center observational study. The relative abundance of gut bacterial taxa on genus level, as well as the microbiome diversity, was correlated to various clinical, laboratory, and histologic parameters. RESULTS: We observed a negative correlation between the model for end-stage liver disease score and Shannon diversity, independent of potentially confounding factors (P(adjust) = 0.046). Alcoholic hepatitis patients with more severe disease had significantly decreased relative abundances of Akkermansia while the relative abundance of Veillonella was increased. We observed a reduction in the Bacteroides abundance (P(adjust) = 0.048) and Shannon diversity (P(adjust) = 0.018) in antibiotic-treated patients and patients receiving steroids had an increase in Veillonella abundance (P(adjust) = 0.005), which was both independent of potentially confounding factors. CONCLUSION: We observed distinct changes in the gut bacterial microbiome of alcoholic hepatitis patients with more severe disease. The gut bacterial microbiome might be an attractive target to prevent and treat this deadly disease.
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spelling pubmed-75243712020-10-06 Changes in the fecal bacterial microbiota associated with disease severity in alcoholic hepatitis patients Lang, Sonja Fairfied, Bradley Gao, Bei Duan, Yi Zhang, Xinlian Fouts, Derrick E. Schnabl, Bernd Gut Microbes Research Paper BACKGROUND AND AIMS: Alcoholic hepatitis is the most severe form of alcohol-related liver disease. While the gut microbiome is known to play a role in disease development and progression, less is known about specific compositional changes of the gut bacterial microbiome associated with disease severity. Therefore, the aim of our study was to correlate gut microbiota features with disease severity in alcoholic hepatitis patients. METHODS: We used 16S rRNA gene sequencing on fecal samples from 74 alcoholic hepatitis patients, which were enrolled at 9 centers in Europe, the United States, and Mexico in a multi-center observational study. The relative abundance of gut bacterial taxa on genus level, as well as the microbiome diversity, was correlated to various clinical, laboratory, and histologic parameters. RESULTS: We observed a negative correlation between the model for end-stage liver disease score and Shannon diversity, independent of potentially confounding factors (P(adjust) = 0.046). Alcoholic hepatitis patients with more severe disease had significantly decreased relative abundances of Akkermansia while the relative abundance of Veillonella was increased. We observed a reduction in the Bacteroides abundance (P(adjust) = 0.048) and Shannon diversity (P(adjust) = 0.018) in antibiotic-treated patients and patients receiving steroids had an increase in Veillonella abundance (P(adjust) = 0.005), which was both independent of potentially confounding factors. CONCLUSION: We observed distinct changes in the gut bacterial microbiome of alcoholic hepatitis patients with more severe disease. The gut bacterial microbiome might be an attractive target to prevent and treat this deadly disease. Taylor & Francis 2020-07-20 /pmc/articles/PMC7524371/ /pubmed/32684075 http://dx.doi.org/10.1080/19490976.2020.1785251 Text en © 2020 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Lang, Sonja
Fairfied, Bradley
Gao, Bei
Duan, Yi
Zhang, Xinlian
Fouts, Derrick E.
Schnabl, Bernd
Changes in the fecal bacterial microbiota associated with disease severity in alcoholic hepatitis patients
title Changes in the fecal bacterial microbiota associated with disease severity in alcoholic hepatitis patients
title_full Changes in the fecal bacterial microbiota associated with disease severity in alcoholic hepatitis patients
title_fullStr Changes in the fecal bacterial microbiota associated with disease severity in alcoholic hepatitis patients
title_full_unstemmed Changes in the fecal bacterial microbiota associated with disease severity in alcoholic hepatitis patients
title_short Changes in the fecal bacterial microbiota associated with disease severity in alcoholic hepatitis patients
title_sort changes in the fecal bacterial microbiota associated with disease severity in alcoholic hepatitis patients
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7524371/
https://www.ncbi.nlm.nih.gov/pubmed/32684075
http://dx.doi.org/10.1080/19490976.2020.1785251
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