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The meconium microbiota shares more features with the amniotic fluid microbiota than the maternal fecal and vaginal microbiota

The early-life gut microbiota is associated with potential development of diseases in adulthood. The sterile womb paradigm has been challenged by recent reports that revealed the presence of the meconium, amniotic fluid, and placenta microbiome. This study aimed to explore the maternal origin of the...

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Autores principales: He, Qiuwen, Kwok, Lai-Yu, Xi, Xiaoxia, Zhong, Zhi, Ma, Teng, Xu, Haiyan, Meng, Haixia, Zhao, Fangqing, Zhang, Heping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7524391/
https://www.ncbi.nlm.nih.gov/pubmed/32744162
http://dx.doi.org/10.1080/19490976.2020.1794266
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author He, Qiuwen
Kwok, Lai-Yu
Xi, Xiaoxia
Zhong, Zhi
Ma, Teng
Xu, Haiyan
Meng, Haixia
Zhao, Fangqing
Zhang, Heping
author_facet He, Qiuwen
Kwok, Lai-Yu
Xi, Xiaoxia
Zhong, Zhi
Ma, Teng
Xu, Haiyan
Meng, Haixia
Zhao, Fangqing
Zhang, Heping
author_sort He, Qiuwen
collection PubMed
description The early-life gut microbiota is associated with potential development of diseases in adulthood. The sterile womb paradigm has been challenged by recent reports that revealed the presence of the meconium, amniotic fluid, and placenta microbiome. This study aimed to explore the maternal origin of the microbiota of neonate meconium by using the PacBio single-molecule real-time circular consensus sequencing technology. Such technology could produce high fidelity reads of full-length 16S rRNA genes, improving the sensitivity and specificity of taxonomic profiling. It also reduced the risk of false positives. This study analyzed the full-length 16S rRNA-based microbiota of maternal samples (amniotic fluid, feces, vaginal fluid, saliva) and first-pass meconium of 39 maternal-neonate pairs. Alpha- and beta-diversity analyses revealed sample type-specific microbiota features. Most sample types were dominated by sequences representing different genera (Lactobacillus and Curvibacter in the amniotic fluid and vaginal fluid microbiota; Bacillus and Escherichia/Shigella in the meconium microbiota; Bacteroides and Faecalibacterium in the maternal fecal microbiota; Streptococcus and Prevotella in the maternal saliva microbiota). Moreover, specific operational taxonomic units (OTUs) were identified in all sample types. Dyad analysis revealed common OTUs between the meconium microbiota and microbiota of multiple maternal samples. The meconium microbiota shared more features with the amniotic fluid microbiota than the maternal fecal and vaginal microbiota. Our results strongly suggested that the meconium microbiota was seeded from multiple maternal body sites, and the amniotic fluid microbiota contributed most to the seeding of the meconium microbiota among the investigated maternal body sites.
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spelling pubmed-75243912020-10-06 The meconium microbiota shares more features with the amniotic fluid microbiota than the maternal fecal and vaginal microbiota He, Qiuwen Kwok, Lai-Yu Xi, Xiaoxia Zhong, Zhi Ma, Teng Xu, Haiyan Meng, Haixia Zhao, Fangqing Zhang, Heping Gut Microbes Research Paper The early-life gut microbiota is associated with potential development of diseases in adulthood. The sterile womb paradigm has been challenged by recent reports that revealed the presence of the meconium, amniotic fluid, and placenta microbiome. This study aimed to explore the maternal origin of the microbiota of neonate meconium by using the PacBio single-molecule real-time circular consensus sequencing technology. Such technology could produce high fidelity reads of full-length 16S rRNA genes, improving the sensitivity and specificity of taxonomic profiling. It also reduced the risk of false positives. This study analyzed the full-length 16S rRNA-based microbiota of maternal samples (amniotic fluid, feces, vaginal fluid, saliva) and first-pass meconium of 39 maternal-neonate pairs. Alpha- and beta-diversity analyses revealed sample type-specific microbiota features. Most sample types were dominated by sequences representing different genera (Lactobacillus and Curvibacter in the amniotic fluid and vaginal fluid microbiota; Bacillus and Escherichia/Shigella in the meconium microbiota; Bacteroides and Faecalibacterium in the maternal fecal microbiota; Streptococcus and Prevotella in the maternal saliva microbiota). Moreover, specific operational taxonomic units (OTUs) were identified in all sample types. Dyad analysis revealed common OTUs between the meconium microbiota and microbiota of multiple maternal samples. The meconium microbiota shared more features with the amniotic fluid microbiota than the maternal fecal and vaginal microbiota. Our results strongly suggested that the meconium microbiota was seeded from multiple maternal body sites, and the amniotic fluid microbiota contributed most to the seeding of the meconium microbiota among the investigated maternal body sites. Taylor & Francis 2020-08-02 /pmc/articles/PMC7524391/ /pubmed/32744162 http://dx.doi.org/10.1080/19490976.2020.1794266 Text en © 2020 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
He, Qiuwen
Kwok, Lai-Yu
Xi, Xiaoxia
Zhong, Zhi
Ma, Teng
Xu, Haiyan
Meng, Haixia
Zhao, Fangqing
Zhang, Heping
The meconium microbiota shares more features with the amniotic fluid microbiota than the maternal fecal and vaginal microbiota
title The meconium microbiota shares more features with the amniotic fluid microbiota than the maternal fecal and vaginal microbiota
title_full The meconium microbiota shares more features with the amniotic fluid microbiota than the maternal fecal and vaginal microbiota
title_fullStr The meconium microbiota shares more features with the amniotic fluid microbiota than the maternal fecal and vaginal microbiota
title_full_unstemmed The meconium microbiota shares more features with the amniotic fluid microbiota than the maternal fecal and vaginal microbiota
title_short The meconium microbiota shares more features with the amniotic fluid microbiota than the maternal fecal and vaginal microbiota
title_sort meconium microbiota shares more features with the amniotic fluid microbiota than the maternal fecal and vaginal microbiota
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7524391/
https://www.ncbi.nlm.nih.gov/pubmed/32744162
http://dx.doi.org/10.1080/19490976.2020.1794266
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