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Dietary fatty acid intake and gut microbiota determine circulating endocannabinoidome signaling beyond the effect of body fat
The endocannabinoidome encompasses several fatty acid (FA)-derived mediators, including the endocannabinoid anandamide (AEA) and 2-arachidonoyl-glycerol (2-AG), which served as targets for anti-obesity drug development, and their congener N-acyl-ethanolamines (NAEs) and 2-monoacyl-glycerols (2‑MAGs)...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7524791/ https://www.ncbi.nlm.nih.gov/pubmed/32994521 http://dx.doi.org/10.1038/s41598-020-72861-3 |
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author | Castonguay-Paradis, Sophie Lacroix, Sébastien Rochefort, Gabrielle Parent, Lydiane Perron, Julie Martin, Cyril Lamarche, Benoît Raymond, Frédéric Flamand, Nicolas Di Marzo, Vincenzo Veilleux, Alain |
author_facet | Castonguay-Paradis, Sophie Lacroix, Sébastien Rochefort, Gabrielle Parent, Lydiane Perron, Julie Martin, Cyril Lamarche, Benoît Raymond, Frédéric Flamand, Nicolas Di Marzo, Vincenzo Veilleux, Alain |
author_sort | Castonguay-Paradis, Sophie |
collection | PubMed |
description | The endocannabinoidome encompasses several fatty acid (FA)-derived mediators, including the endocannabinoid anandamide (AEA) and 2-arachidonoyl-glycerol (2-AG), which served as targets for anti-obesity drug development, and their congener N-acyl-ethanolamines (NAEs) and 2-monoacyl-glycerols (2‑MAGs), which are involved in food intake and energy metabolism. Body weight and fat distribution have been suggested as determinants of peripheral endocannabinoid levels. We aimed at investigating factors, beyond body fat composition, that are associated with circulating NAE and 2-MAG levels in a heterogeneous human population. Plasma NAEs and 2-MAGs were measured using LC–MS/MS in a cross-sectional sample of healthy men and women (n = 195) covering a wide range of BMI and individuals before and after a 2-day Mediterranean diet (n = 21). Circulating levels of all 2-MAGs and NAEs, other than N-oleoyl-ethanolamine (OEA), correlated with body fat mass and visceral adipose tissue (0.26 < r < 0.54). NAE levels were elevated in individuals with elevated fat mass, while 2-MAGs were increased in individuals with predominantly visceral body fat distribution. Dietary intakes of specific FAs were associated with 2-AG and omega-3-FA-derived NAEs or 2-MAGs, irrespective of the body fat distribution. Some gut bacterial families (e.g. Veillonellaceae, Peptostreptococcaceae and Akkermansiaceae) were associated with variations in most NAEs or omega-3-FA-derived 2‑MAGs, independently of fat mass and dietary FA intake. Finally, a 2-day Mediterranean diet intervention increased circulating levels of NAEs and 2-MAGs in agreement with changes in FA intake (p < 0.01). Self-reported intake and short-term dietary intervention increased in oleic acid and EPA and DHA intake as well as certain gut microbiota taxa are associated to circulating NAEs and 2‑MAGs independently of adiposity measures, thus highlighting the potential importance of these variables in determining endocannabinoidome signaling in humans. |
format | Online Article Text |
id | pubmed-7524791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-75247912020-10-01 Dietary fatty acid intake and gut microbiota determine circulating endocannabinoidome signaling beyond the effect of body fat Castonguay-Paradis, Sophie Lacroix, Sébastien Rochefort, Gabrielle Parent, Lydiane Perron, Julie Martin, Cyril Lamarche, Benoît Raymond, Frédéric Flamand, Nicolas Di Marzo, Vincenzo Veilleux, Alain Sci Rep Article The endocannabinoidome encompasses several fatty acid (FA)-derived mediators, including the endocannabinoid anandamide (AEA) and 2-arachidonoyl-glycerol (2-AG), which served as targets for anti-obesity drug development, and their congener N-acyl-ethanolamines (NAEs) and 2-monoacyl-glycerols (2‑MAGs), which are involved in food intake and energy metabolism. Body weight and fat distribution have been suggested as determinants of peripheral endocannabinoid levels. We aimed at investigating factors, beyond body fat composition, that are associated with circulating NAE and 2-MAG levels in a heterogeneous human population. Plasma NAEs and 2-MAGs were measured using LC–MS/MS in a cross-sectional sample of healthy men and women (n = 195) covering a wide range of BMI and individuals before and after a 2-day Mediterranean diet (n = 21). Circulating levels of all 2-MAGs and NAEs, other than N-oleoyl-ethanolamine (OEA), correlated with body fat mass and visceral adipose tissue (0.26 < r < 0.54). NAE levels were elevated in individuals with elevated fat mass, while 2-MAGs were increased in individuals with predominantly visceral body fat distribution. Dietary intakes of specific FAs were associated with 2-AG and omega-3-FA-derived NAEs or 2-MAGs, irrespective of the body fat distribution. Some gut bacterial families (e.g. Veillonellaceae, Peptostreptococcaceae and Akkermansiaceae) were associated with variations in most NAEs or omega-3-FA-derived 2‑MAGs, independently of fat mass and dietary FA intake. Finally, a 2-day Mediterranean diet intervention increased circulating levels of NAEs and 2-MAGs in agreement with changes in FA intake (p < 0.01). Self-reported intake and short-term dietary intervention increased in oleic acid and EPA and DHA intake as well as certain gut microbiota taxa are associated to circulating NAEs and 2‑MAGs independently of adiposity measures, thus highlighting the potential importance of these variables in determining endocannabinoidome signaling in humans. Nature Publishing Group UK 2020-09-29 /pmc/articles/PMC7524791/ /pubmed/32994521 http://dx.doi.org/10.1038/s41598-020-72861-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Castonguay-Paradis, Sophie Lacroix, Sébastien Rochefort, Gabrielle Parent, Lydiane Perron, Julie Martin, Cyril Lamarche, Benoît Raymond, Frédéric Flamand, Nicolas Di Marzo, Vincenzo Veilleux, Alain Dietary fatty acid intake and gut microbiota determine circulating endocannabinoidome signaling beyond the effect of body fat |
title | Dietary fatty acid intake and gut microbiota determine circulating endocannabinoidome signaling beyond the effect of body fat |
title_full | Dietary fatty acid intake and gut microbiota determine circulating endocannabinoidome signaling beyond the effect of body fat |
title_fullStr | Dietary fatty acid intake and gut microbiota determine circulating endocannabinoidome signaling beyond the effect of body fat |
title_full_unstemmed | Dietary fatty acid intake and gut microbiota determine circulating endocannabinoidome signaling beyond the effect of body fat |
title_short | Dietary fatty acid intake and gut microbiota determine circulating endocannabinoidome signaling beyond the effect of body fat |
title_sort | dietary fatty acid intake and gut microbiota determine circulating endocannabinoidome signaling beyond the effect of body fat |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7524791/ https://www.ncbi.nlm.nih.gov/pubmed/32994521 http://dx.doi.org/10.1038/s41598-020-72861-3 |
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