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Characterization of a new case of XMLV (Bxv1) contamination in the human cell line Hep2 (clone 2B)
The use of misidentified cell lines contaminated by other cell lines and/or microorganisms has generated much confusion in the scientific literature. Detailed characterization of such contaminations is therefore crucial to avoid misinterpretation and ensure robustness and reproducibility of research...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7524804/ https://www.ncbi.nlm.nih.gov/pubmed/32994520 http://dx.doi.org/10.1038/s41598-020-73169-y |
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author | Loiseau, Vincent Cordaux, Richard Giraud, Isabelle Beby-Defaux, Agnès Lévêque, Nicolas Gilbert, Clément |
author_facet | Loiseau, Vincent Cordaux, Richard Giraud, Isabelle Beby-Defaux, Agnès Lévêque, Nicolas Gilbert, Clément |
author_sort | Loiseau, Vincent |
collection | PubMed |
description | The use of misidentified cell lines contaminated by other cell lines and/or microorganisms has generated much confusion in the scientific literature. Detailed characterization of such contaminations is therefore crucial to avoid misinterpretation and ensure robustness and reproducibility of research. Here we use DNA-seq data produced in our lab to first confirm that the Hep2 (clone 2B) cell line (Sigma-Aldrich catalog number: 85011412-1VL) is indistinguishable from the HeLa cell line by mapping integrations of the human papillomavirus 18 (HPV18) at their expected loci on chromosome 8. We then show that the cell line is also contaminated by a xenotropic murine leukemia virus (XMLV) that is nearly identical to the mouse Bxv1 provirus and we characterize one Bxv1 provirus, located in the second intron of the pseudouridylate synthase 1 (PUS1) gene. Using an RNA-seq dataset, we confirm the high expression of the E6 and E7 HPV18 oncogenes, show that the entire Bxv1 genome is moderately expressed, and retrieve a Bxv1 splicing event favouring expression of the env gene. Hep2 (clone 2B) is the fourth human cell line so far known to be contaminated by the Bxv1 XMLV. This contamination has to be taken into account when using the cell line in future experiments. |
format | Online Article Text |
id | pubmed-7524804 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-75248042020-10-01 Characterization of a new case of XMLV (Bxv1) contamination in the human cell line Hep2 (clone 2B) Loiseau, Vincent Cordaux, Richard Giraud, Isabelle Beby-Defaux, Agnès Lévêque, Nicolas Gilbert, Clément Sci Rep Article The use of misidentified cell lines contaminated by other cell lines and/or microorganisms has generated much confusion in the scientific literature. Detailed characterization of such contaminations is therefore crucial to avoid misinterpretation and ensure robustness and reproducibility of research. Here we use DNA-seq data produced in our lab to first confirm that the Hep2 (clone 2B) cell line (Sigma-Aldrich catalog number: 85011412-1VL) is indistinguishable from the HeLa cell line by mapping integrations of the human papillomavirus 18 (HPV18) at their expected loci on chromosome 8. We then show that the cell line is also contaminated by a xenotropic murine leukemia virus (XMLV) that is nearly identical to the mouse Bxv1 provirus and we characterize one Bxv1 provirus, located in the second intron of the pseudouridylate synthase 1 (PUS1) gene. Using an RNA-seq dataset, we confirm the high expression of the E6 and E7 HPV18 oncogenes, show that the entire Bxv1 genome is moderately expressed, and retrieve a Bxv1 splicing event favouring expression of the env gene. Hep2 (clone 2B) is the fourth human cell line so far known to be contaminated by the Bxv1 XMLV. This contamination has to be taken into account when using the cell line in future experiments. Nature Publishing Group UK 2020-09-29 /pmc/articles/PMC7524804/ /pubmed/32994520 http://dx.doi.org/10.1038/s41598-020-73169-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Loiseau, Vincent Cordaux, Richard Giraud, Isabelle Beby-Defaux, Agnès Lévêque, Nicolas Gilbert, Clément Characterization of a new case of XMLV (Bxv1) contamination in the human cell line Hep2 (clone 2B) |
title | Characterization of a new case of XMLV (Bxv1) contamination in the human cell line Hep2 (clone 2B) |
title_full | Characterization of a new case of XMLV (Bxv1) contamination in the human cell line Hep2 (clone 2B) |
title_fullStr | Characterization of a new case of XMLV (Bxv1) contamination in the human cell line Hep2 (clone 2B) |
title_full_unstemmed | Characterization of a new case of XMLV (Bxv1) contamination in the human cell line Hep2 (clone 2B) |
title_short | Characterization of a new case of XMLV (Bxv1) contamination in the human cell line Hep2 (clone 2B) |
title_sort | characterization of a new case of xmlv (bxv1) contamination in the human cell line hep2 (clone 2b) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7524804/ https://www.ncbi.nlm.nih.gov/pubmed/32994520 http://dx.doi.org/10.1038/s41598-020-73169-y |
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