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Cellular Base of Mint Allelopathy: Menthone Affects Plant Microtubules

Plants can use volatiles for remote suppression of competitors. Mints produce essential oils, which are known to affect the growth of other plants. We used a comparative approach to identify allelopathic compounds from different Mints (genus Mentha, but also including Cat Mint, Nepeta cataria, and C...

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Autores principales: Sarheed, Mohammed Mahmood, Rajabi, Fatemeh, Kunert, Maritta, Boland, Wilhelm, Wetters, Sascha, Miadowitz, Kai, Kaźmierczak, Andrzej, Sahi, Vaidurya Pratap, Nick, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7524878/
https://www.ncbi.nlm.nih.gov/pubmed/33042176
http://dx.doi.org/10.3389/fpls.2020.546345
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author Sarheed, Mohammed Mahmood
Rajabi, Fatemeh
Kunert, Maritta
Boland, Wilhelm
Wetters, Sascha
Miadowitz, Kai
Kaźmierczak, Andrzej
Sahi, Vaidurya Pratap
Nick, Peter
author_facet Sarheed, Mohammed Mahmood
Rajabi, Fatemeh
Kunert, Maritta
Boland, Wilhelm
Wetters, Sascha
Miadowitz, Kai
Kaźmierczak, Andrzej
Sahi, Vaidurya Pratap
Nick, Peter
author_sort Sarheed, Mohammed Mahmood
collection PubMed
description Plants can use volatiles for remote suppression of competitors. Mints produce essential oils, which are known to affect the growth of other plants. We used a comparative approach to identify allelopathic compounds from different Mints (genus Mentha, but also including Cat Mint, Nepeta cataria, and Corean Mint, Agastache rugosa, belonging to sisters clades within the Mentheae) using the standard cress germination assay as readout. To understand the mechanism behind this allelopathic effect, we investigated the response of tobacco BY-2 cell lines, expressing GFP-tagged markers for microtubules and actin filaments to these essential oils. Based on the comparison between bioactivity and chemical components, we identified menthone as prime candidate for the allelopathic effect, and confirmed this bioactivity targeted to microtubules experimentally in both, plant cells (tobaccoBY-2), and seedlings (Arabidopsis thaliana). We could show that menthone disrupted microtubules and induced mortality linked with a rapid permeabilization (less than 15 min) of the plasma membrane. This mortality was elevated in a tubulin marker line, where microtubules are mildly stabilized. Our study paves the way for the development of novel bioherbicides that would be environmentally friendly.
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spelling pubmed-75248782020-10-09 Cellular Base of Mint Allelopathy: Menthone Affects Plant Microtubules Sarheed, Mohammed Mahmood Rajabi, Fatemeh Kunert, Maritta Boland, Wilhelm Wetters, Sascha Miadowitz, Kai Kaźmierczak, Andrzej Sahi, Vaidurya Pratap Nick, Peter Front Plant Sci Plant Science Plants can use volatiles for remote suppression of competitors. Mints produce essential oils, which are known to affect the growth of other plants. We used a comparative approach to identify allelopathic compounds from different Mints (genus Mentha, but also including Cat Mint, Nepeta cataria, and Corean Mint, Agastache rugosa, belonging to sisters clades within the Mentheae) using the standard cress germination assay as readout. To understand the mechanism behind this allelopathic effect, we investigated the response of tobacco BY-2 cell lines, expressing GFP-tagged markers for microtubules and actin filaments to these essential oils. Based on the comparison between bioactivity and chemical components, we identified menthone as prime candidate for the allelopathic effect, and confirmed this bioactivity targeted to microtubules experimentally in both, plant cells (tobaccoBY-2), and seedlings (Arabidopsis thaliana). We could show that menthone disrupted microtubules and induced mortality linked with a rapid permeabilization (less than 15 min) of the plasma membrane. This mortality was elevated in a tubulin marker line, where microtubules are mildly stabilized. Our study paves the way for the development of novel bioherbicides that would be environmentally friendly. Frontiers Media S.A. 2020-09-16 /pmc/articles/PMC7524878/ /pubmed/33042176 http://dx.doi.org/10.3389/fpls.2020.546345 Text en Copyright © 2020 Sarheed, Rajabi, Kunert, Boland, Wetters, Miadowitz, Kaźmierczak, Sahi and Nick http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Sarheed, Mohammed Mahmood
Rajabi, Fatemeh
Kunert, Maritta
Boland, Wilhelm
Wetters, Sascha
Miadowitz, Kai
Kaźmierczak, Andrzej
Sahi, Vaidurya Pratap
Nick, Peter
Cellular Base of Mint Allelopathy: Menthone Affects Plant Microtubules
title Cellular Base of Mint Allelopathy: Menthone Affects Plant Microtubules
title_full Cellular Base of Mint Allelopathy: Menthone Affects Plant Microtubules
title_fullStr Cellular Base of Mint Allelopathy: Menthone Affects Plant Microtubules
title_full_unstemmed Cellular Base of Mint Allelopathy: Menthone Affects Plant Microtubules
title_short Cellular Base of Mint Allelopathy: Menthone Affects Plant Microtubules
title_sort cellular base of mint allelopathy: menthone affects plant microtubules
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7524878/
https://www.ncbi.nlm.nih.gov/pubmed/33042176
http://dx.doi.org/10.3389/fpls.2020.546345
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