An Electrochemical Sandwich Immunosensor Based on Signal Amplification Technique for the Determination of Alpha-Fetoprotein

The synthesis of Au nanocubes is used to label alpha-fetoprotein antibody (anti-AFP) and horseradish peroxidase (HRP) to form an immune complex for antibody detection. Graphene oxide-methylene blue-gold nanoparticles (GO-MB-AuNPs) nanocomposites were used as the immunosensing platform. This proposed...

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Autores principales: Shen, Changming, Wang, Lin, Zhang, Hongyan, Liu, Shaojuan, Jiang, Jianwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7525061/
https://www.ncbi.nlm.nih.gov/pubmed/33195093
http://dx.doi.org/10.3389/fchem.2020.589560
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author Shen, Changming
Wang, Lin
Zhang, Hongyan
Liu, Shaojuan
Jiang, Jianwei
author_facet Shen, Changming
Wang, Lin
Zhang, Hongyan
Liu, Shaojuan
Jiang, Jianwei
author_sort Shen, Changming
collection PubMed
description The synthesis of Au nanocubes is used to label alpha-fetoprotein antibody (anti-AFP) and horseradish peroxidase (HRP) to form an immune complex for antibody detection. Graphene oxide-methylene blue-gold nanoparticles (GO-MB-AuNPs) nanocomposites were used as the immunosensing platform. This proposed sandwich-type immunoassay shows good performance. This method establishes a feasible amperometric immunoassay method for sensitive analysis of AFP in serum samples. Under the optimal experimental conditions, the DPV current response of the immunosensor is proportional to the logarithmic value of the AFP concentration. The linear detection range can achieve to 0.005–20 ng/mL with a detection limit of 1.5 pg/mL. The proposed immunosensor has good precision, selectivity and stability, and can be used for AFP determination in clinical tests.
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spelling pubmed-75250612020-11-13 An Electrochemical Sandwich Immunosensor Based on Signal Amplification Technique for the Determination of Alpha-Fetoprotein Shen, Changming Wang, Lin Zhang, Hongyan Liu, Shaojuan Jiang, Jianwei Front Chem Chemistry The synthesis of Au nanocubes is used to label alpha-fetoprotein antibody (anti-AFP) and horseradish peroxidase (HRP) to form an immune complex for antibody detection. Graphene oxide-methylene blue-gold nanoparticles (GO-MB-AuNPs) nanocomposites were used as the immunosensing platform. This proposed sandwich-type immunoassay shows good performance. This method establishes a feasible amperometric immunoassay method for sensitive analysis of AFP in serum samples. Under the optimal experimental conditions, the DPV current response of the immunosensor is proportional to the logarithmic value of the AFP concentration. The linear detection range can achieve to 0.005–20 ng/mL with a detection limit of 1.5 pg/mL. The proposed immunosensor has good precision, selectivity and stability, and can be used for AFP determination in clinical tests. Frontiers Media S.A. 2020-09-16 /pmc/articles/PMC7525061/ /pubmed/33195093 http://dx.doi.org/10.3389/fchem.2020.589560 Text en Copyright © 2020 Shen, Wang, Zhang, Liu and Jiang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Shen, Changming
Wang, Lin
Zhang, Hongyan
Liu, Shaojuan
Jiang, Jianwei
An Electrochemical Sandwich Immunosensor Based on Signal Amplification Technique for the Determination of Alpha-Fetoprotein
title An Electrochemical Sandwich Immunosensor Based on Signal Amplification Technique for the Determination of Alpha-Fetoprotein
title_full An Electrochemical Sandwich Immunosensor Based on Signal Amplification Technique for the Determination of Alpha-Fetoprotein
title_fullStr An Electrochemical Sandwich Immunosensor Based on Signal Amplification Technique for the Determination of Alpha-Fetoprotein
title_full_unstemmed An Electrochemical Sandwich Immunosensor Based on Signal Amplification Technique for the Determination of Alpha-Fetoprotein
title_short An Electrochemical Sandwich Immunosensor Based on Signal Amplification Technique for the Determination of Alpha-Fetoprotein
title_sort electrochemical sandwich immunosensor based on signal amplification technique for the determination of alpha-fetoprotein
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7525061/
https://www.ncbi.nlm.nih.gov/pubmed/33195093
http://dx.doi.org/10.3389/fchem.2020.589560
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