Cargando…

The effect of anti-tuberculosis drugs therapy on mRNA efflux pump gene expression of Rv1250 in Mycobacterium tuberculosis collected from tuberculosis patients

Efflux pumps are transmembrane proteins that vigorously participate in extruding a wide range of substrates, including drugs, outside the bacterial cell. We aimed to investigate the mRNA expression level of the Rv1250 efflux pump gene in Mycobacterium tuberculosis isolated from individuals with tube...

Descripción completa

Detalles Bibliográficos
Autores principales: Umar, F., Hatta, M., Husain, D.R., Natzir, R., Dwiyanti, R., Junita, A.R., Primaguna, M.R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7525134/
https://www.ncbi.nlm.nih.gov/pubmed/33014381
http://dx.doi.org/10.1016/j.nmni.2019.100609
_version_ 1783588679996932096
author Umar, F.
Hatta, M.
Husain, D.R.
Natzir, R.
Dwiyanti, R.
Junita, A.R.
Primaguna, M.R.
author_facet Umar, F.
Hatta, M.
Husain, D.R.
Natzir, R.
Dwiyanti, R.
Junita, A.R.
Primaguna, M.R.
author_sort Umar, F.
collection PubMed
description Efflux pumps are transmembrane proteins that vigorously participate in extruding a wide range of substrates, including drugs, outside the bacterial cell. We aimed to investigate the mRNA expression level of the Rv1250 efflux pump gene in Mycobacterium tuberculosis isolated from individuals with tuberculosis who received drug therapy, at the 1st, 3rd and 5th months, and newly diagnosed patients with tuberculosis who will receive drug therapy (0 month). The study was a multiple cross-sectional longitudinal design—50 different M. tuberculosis isolates and a reference strain H37Rv were subcultured in LJ medium and confirmed by multiplex PCR for identification of M. tuberculosis and collected for RNA extraction. Total bacterial mRNA was analysed using real-time quantitative PCR to evaluate mRNA quantification gene expression. There were differences in the level of Rv1250 mRNA expression between sensitive (n = 11) and resistant (n = 40) groups of 5.961 ± 0.414 and 10.192 ± 1.978, respectively (fold changes; p < 0.05). There were significant differences of expression level among M. tuberculosis-resistant groups (p < 0.05) specifically 7.573 ± 0.424 for 0-month drug therapy (n = 10), 9.438 ± 0.644 for 1st month drug therapy (n = 10), 11.057 ± 0.262 for 3rd month drug therapy (n = 10) and 12.701 ± 0.460 for 5th month drug therapy (n = 10). We assume that the extent of Rv1250 gene expression in M. tuberculosis clinical isolates is a result of the exposure to antimicrobials during treatment, which affect the basic expression of the efflux pump Rv1250 gene.
format Online
Article
Text
id pubmed-7525134
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-75251342020-10-02 The effect of anti-tuberculosis drugs therapy on mRNA efflux pump gene expression of Rv1250 in Mycobacterium tuberculosis collected from tuberculosis patients Umar, F. Hatta, M. Husain, D.R. Natzir, R. Dwiyanti, R. Junita, A.R. Primaguna, M.R. New Microbes New Infect New Resistant Microbes in Humans Efflux pumps are transmembrane proteins that vigorously participate in extruding a wide range of substrates, including drugs, outside the bacterial cell. We aimed to investigate the mRNA expression level of the Rv1250 efflux pump gene in Mycobacterium tuberculosis isolated from individuals with tuberculosis who received drug therapy, at the 1st, 3rd and 5th months, and newly diagnosed patients with tuberculosis who will receive drug therapy (0 month). The study was a multiple cross-sectional longitudinal design—50 different M. tuberculosis isolates and a reference strain H37Rv were subcultured in LJ medium and confirmed by multiplex PCR for identification of M. tuberculosis and collected for RNA extraction. Total bacterial mRNA was analysed using real-time quantitative PCR to evaluate mRNA quantification gene expression. There were differences in the level of Rv1250 mRNA expression between sensitive (n = 11) and resistant (n = 40) groups of 5.961 ± 0.414 and 10.192 ± 1.978, respectively (fold changes; p < 0.05). There were significant differences of expression level among M. tuberculosis-resistant groups (p < 0.05) specifically 7.573 ± 0.424 for 0-month drug therapy (n = 10), 9.438 ± 0.644 for 1st month drug therapy (n = 10), 11.057 ± 0.262 for 3rd month drug therapy (n = 10) and 12.701 ± 0.460 for 5th month drug therapy (n = 10). We assume that the extent of Rv1250 gene expression in M. tuberculosis clinical isolates is a result of the exposure to antimicrobials during treatment, which affect the basic expression of the efflux pump Rv1250 gene. Elsevier 2019-10-08 /pmc/articles/PMC7525134/ /pubmed/33014381 http://dx.doi.org/10.1016/j.nmni.2019.100609 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle New Resistant Microbes in Humans
Umar, F.
Hatta, M.
Husain, D.R.
Natzir, R.
Dwiyanti, R.
Junita, A.R.
Primaguna, M.R.
The effect of anti-tuberculosis drugs therapy on mRNA efflux pump gene expression of Rv1250 in Mycobacterium tuberculosis collected from tuberculosis patients
title The effect of anti-tuberculosis drugs therapy on mRNA efflux pump gene expression of Rv1250 in Mycobacterium tuberculosis collected from tuberculosis patients
title_full The effect of anti-tuberculosis drugs therapy on mRNA efflux pump gene expression of Rv1250 in Mycobacterium tuberculosis collected from tuberculosis patients
title_fullStr The effect of anti-tuberculosis drugs therapy on mRNA efflux pump gene expression of Rv1250 in Mycobacterium tuberculosis collected from tuberculosis patients
title_full_unstemmed The effect of anti-tuberculosis drugs therapy on mRNA efflux pump gene expression of Rv1250 in Mycobacterium tuberculosis collected from tuberculosis patients
title_short The effect of anti-tuberculosis drugs therapy on mRNA efflux pump gene expression of Rv1250 in Mycobacterium tuberculosis collected from tuberculosis patients
title_sort effect of anti-tuberculosis drugs therapy on mrna efflux pump gene expression of rv1250 in mycobacterium tuberculosis collected from tuberculosis patients
topic New Resistant Microbes in Humans
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7525134/
https://www.ncbi.nlm.nih.gov/pubmed/33014381
http://dx.doi.org/10.1016/j.nmni.2019.100609
work_keys_str_mv AT umarf theeffectofantituberculosisdrugstherapyonmrnaeffluxpumpgeneexpressionofrv1250inmycobacteriumtuberculosiscollectedfromtuberculosispatients
AT hattam theeffectofantituberculosisdrugstherapyonmrnaeffluxpumpgeneexpressionofrv1250inmycobacteriumtuberculosiscollectedfromtuberculosispatients
AT husaindr theeffectofantituberculosisdrugstherapyonmrnaeffluxpumpgeneexpressionofrv1250inmycobacteriumtuberculosiscollectedfromtuberculosispatients
AT natzirr theeffectofantituberculosisdrugstherapyonmrnaeffluxpumpgeneexpressionofrv1250inmycobacteriumtuberculosiscollectedfromtuberculosispatients
AT dwiyantir theeffectofantituberculosisdrugstherapyonmrnaeffluxpumpgeneexpressionofrv1250inmycobacteriumtuberculosiscollectedfromtuberculosispatients
AT junitaar theeffectofantituberculosisdrugstherapyonmrnaeffluxpumpgeneexpressionofrv1250inmycobacteriumtuberculosiscollectedfromtuberculosispatients
AT primagunamr theeffectofantituberculosisdrugstherapyonmrnaeffluxpumpgeneexpressionofrv1250inmycobacteriumtuberculosiscollectedfromtuberculosispatients
AT umarf effectofantituberculosisdrugstherapyonmrnaeffluxpumpgeneexpressionofrv1250inmycobacteriumtuberculosiscollectedfromtuberculosispatients
AT hattam effectofantituberculosisdrugstherapyonmrnaeffluxpumpgeneexpressionofrv1250inmycobacteriumtuberculosiscollectedfromtuberculosispatients
AT husaindr effectofantituberculosisdrugstherapyonmrnaeffluxpumpgeneexpressionofrv1250inmycobacteriumtuberculosiscollectedfromtuberculosispatients
AT natzirr effectofantituberculosisdrugstherapyonmrnaeffluxpumpgeneexpressionofrv1250inmycobacteriumtuberculosiscollectedfromtuberculosispatients
AT dwiyantir effectofantituberculosisdrugstherapyonmrnaeffluxpumpgeneexpressionofrv1250inmycobacteriumtuberculosiscollectedfromtuberculosispatients
AT junitaar effectofantituberculosisdrugstherapyonmrnaeffluxpumpgeneexpressionofrv1250inmycobacteriumtuberculosiscollectedfromtuberculosispatients
AT primagunamr effectofantituberculosisdrugstherapyonmrnaeffluxpumpgeneexpressionofrv1250inmycobacteriumtuberculosiscollectedfromtuberculosispatients