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Myelin replacement triggered by single-cell demyelination in mouse cortex
Myelin, rather than being a static insulator of axons, is emerging as an active participant in circuit plasticity. This requires precise regulation of oligodendrocyte numbers and myelination patterns. Here, by devising a laser ablation approach of single oligodendrocytes, followed by in vivo imaging...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7525521/ https://www.ncbi.nlm.nih.gov/pubmed/32994410 http://dx.doi.org/10.1038/s41467-020-18632-0 |
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author | Snaidero, Nicolas Schifferer, Martina Mezydlo, Aleksandra Zalc, Bernard Kerschensteiner, Martin Misgeld, Thomas |
author_facet | Snaidero, Nicolas Schifferer, Martina Mezydlo, Aleksandra Zalc, Bernard Kerschensteiner, Martin Misgeld, Thomas |
author_sort | Snaidero, Nicolas |
collection | PubMed |
description | Myelin, rather than being a static insulator of axons, is emerging as an active participant in circuit plasticity. This requires precise regulation of oligodendrocyte numbers and myelination patterns. Here, by devising a laser ablation approach of single oligodendrocytes, followed by in vivo imaging and correlated ultrastructural reconstructions, we report that in mouse cortex demyelination as subtle as the loss of a single oligodendrocyte can trigger robust cell replacement and remyelination timed by myelin breakdown. This results in reliable reestablishment of the original myelin pattern along continuously myelinated axons, while in parallel, patchy isolated internodes emerge on previously unmyelinated axons. Therefore, in mammalian cortex, internodes along partially myelinated cortical axons are typically not reestablished, suggesting that the cues that guide patchy myelination are not preserved through cycles of de- and remyelination. In contrast, myelin sheaths forming continuous patterns show remarkable homeostatic resilience and remyelinate with single axon precision. |
format | Online Article Text |
id | pubmed-7525521 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-75255212020-10-19 Myelin replacement triggered by single-cell demyelination in mouse cortex Snaidero, Nicolas Schifferer, Martina Mezydlo, Aleksandra Zalc, Bernard Kerschensteiner, Martin Misgeld, Thomas Nat Commun Article Myelin, rather than being a static insulator of axons, is emerging as an active participant in circuit plasticity. This requires precise regulation of oligodendrocyte numbers and myelination patterns. Here, by devising a laser ablation approach of single oligodendrocytes, followed by in vivo imaging and correlated ultrastructural reconstructions, we report that in mouse cortex demyelination as subtle as the loss of a single oligodendrocyte can trigger robust cell replacement and remyelination timed by myelin breakdown. This results in reliable reestablishment of the original myelin pattern along continuously myelinated axons, while in parallel, patchy isolated internodes emerge on previously unmyelinated axons. Therefore, in mammalian cortex, internodes along partially myelinated cortical axons are typically not reestablished, suggesting that the cues that guide patchy myelination are not preserved through cycles of de- and remyelination. In contrast, myelin sheaths forming continuous patterns show remarkable homeostatic resilience and remyelinate with single axon precision. Nature Publishing Group UK 2020-09-29 /pmc/articles/PMC7525521/ /pubmed/32994410 http://dx.doi.org/10.1038/s41467-020-18632-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Snaidero, Nicolas Schifferer, Martina Mezydlo, Aleksandra Zalc, Bernard Kerschensteiner, Martin Misgeld, Thomas Myelin replacement triggered by single-cell demyelination in mouse cortex |
title | Myelin replacement triggered by single-cell demyelination in mouse cortex |
title_full | Myelin replacement triggered by single-cell demyelination in mouse cortex |
title_fullStr | Myelin replacement triggered by single-cell demyelination in mouse cortex |
title_full_unstemmed | Myelin replacement triggered by single-cell demyelination in mouse cortex |
title_short | Myelin replacement triggered by single-cell demyelination in mouse cortex |
title_sort | myelin replacement triggered by single-cell demyelination in mouse cortex |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7525521/ https://www.ncbi.nlm.nih.gov/pubmed/32994410 http://dx.doi.org/10.1038/s41467-020-18632-0 |
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