Microtubule nucleation promoters Mto1 and Mto2 regulate cytokinesis in fission yeast

Microtubules of the mitotic spindle direct cytokinesis in metazoans but this has not been documented in fungi. We report evidence that microtubule nucleators at the spindle pole body help coordinate cytokinetic furrow formation in fission yeast. The temperature-sensitive cps1-191 strain (Liu et al., 1999) with a D277N substitution in β-glucan synthase 1 (Cps1/Bgs1) was reported to arrest with an unconstricted contractile ring. We discovered that contractile rings in cps1-191 cells constrict slowly and that an mto2(S338N) mutation is required with the bgs1(D277N)mutation to reproduce the cps1-191 phenotype. Complexes of Mto2 and Mto1 with γ-tubulin regulate microtubule assembly. Deletion of Mto1 along with the bgs1(D277N) mutation also gives the cps1-191 phenotype, which is not observed in mto2(S338N) or mto1Δ cells expressing bgs1(+). Both mto2(S338N) and mto1Δ cells nucleate fewer astral microtubules than normal and have higher levels of Rho1-GTP at the division site than wild-type cells. We report multiple conditions that sensitize mto1Δ and mto2(S338N) cells to furrow ingression phenotypes.