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Changes in the proteomic profile of blood serum in coronary atherosclerosis

BACKGROUND: Our aim was to study changes in the serum proteomic profile in coronary atherosclerosis. METHODS: The study involved two groups of patients: 1) men with coronary heart disease and coronary atherosclerosis (n = 15); 2) control (n = 15): men without coronary heart disease. The object of th...

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Detalles Bibliográficos
Autores principales: Stakhneva, Ekaterina M., Meshcheryakova, Irina A., Demidov, Evgeny A., Starostin, Konstantin V., Peltek, Sergey E., Voevoda, Michael I., Ragino, Yuliya I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Medical Biochemists of Serbia, Belgrade 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7526017/
https://www.ncbi.nlm.nih.gov/pubmed/33033454
http://dx.doi.org/10.2478/jomb-2019-0022
Descripción
Sumario:BACKGROUND: Our aim was to study changes in the serum proteomic profile in coronary atherosclerosis. METHODS: The study involved two groups of patients: 1) men with coronary heart disease and coronary atherosclerosis (n = 15); 2) control (n = 15): men without coronary heart disease. The object of this study was blood serum. Separation of proteins for the investigation of differences in serum protein components was performed by two-dimensional electrophoresis. Identification of protein fractions was carried out using peptide mass maps by the matrix-assisted laser desorption ionization method. RESULTS: In blood serum samples from patients with coronary atherosclerosis, protein separation in two-dimensional gels with mass-spectrometric identification revealed an increase of some proteins: hemopexin, transthyretin (monomeric form), retinol-binding protein 4, and components of the complement system: C3 (chain B) and C9. There was a decrease of some proteins: kininogen, zinc finger protein 133, and B-cell CLL/lymphoma 6 member B protein. Comparisons between the experimental and control group were carried out in protein fractions where the protein amount differed more than 1.5-fold (p < 0.05). CONCLUSIONS: Proteome profiling of serum revealed a change in the content of kininogen, hemopexin, transthyretin, retinol-binding protein, and proteins of the complement system (C9, and C3) in coronary atherosclerosis. The contribution to the differential expression of a protein was often made by isoforms of the protein, particularly transthyretin. The change in the concentrations of functionally interacting proteins, such as transthyretin and retinol-binding protein, were noted.