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Comparing methods for controlled capture and quantification of pollen in Cannabis sativa

PREMISE: Precise pollen collection methods are necessary for crop breeding, but anemophilous pollen is notoriously difficult to capture and control. Here we compared a variety of methods for the controlled capture of cannabis pollen, intended to ease the process of cross‐fertilization for breeding t...

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Detalles Bibliográficos
Autores principales: Wizenberg, Sydney B., Weis, Arthur E., Campbell, Lesley G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7526430/
https://www.ncbi.nlm.nih.gov/pubmed/33014633
http://dx.doi.org/10.1002/aps3.11389
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author Wizenberg, Sydney B.
Weis, Arthur E.
Campbell, Lesley G.
author_facet Wizenberg, Sydney B.
Weis, Arthur E.
Campbell, Lesley G.
author_sort Wizenberg, Sydney B.
collection PubMed
description PREMISE: Precise pollen collection methods are necessary for crop breeding, but anemophilous pollen is notoriously difficult to capture and control. Here we compared a variety of methods for the controlled capture of cannabis pollen, intended to ease the process of cross‐fertilization for breeding this wind‐pollinated plant, and measured the utility of light spectroscopy for quantifying relative pollen yield. METHODS AND RESULTS: In two independent trials, we compared a control method of pollen collection (hand collection) to either vacuum‐, water‐, or bag‐collection methods. We used visible light spectroscopy to quantify relative pollen yield, and validated this approach using microscopic pollen counts. We determined that pollen yield was highest when using hand collection or vacuum collection, but efficiency did not differ significantly among methods. CONCLUSIONS: To maximize yield, pollen should be collected by hand or vacuum, but all collection methods were equally efficient in a relative sense because yield increased with collection time. We also found that light spectroscopy is an accurate and rapid method of quantifying pollen abundance (R (2) = 0.86) in a liquid suspension.
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spelling pubmed-75264302020-10-02 Comparing methods for controlled capture and quantification of pollen in Cannabis sativa Wizenberg, Sydney B. Weis, Arthur E. Campbell, Lesley G. Appl Plant Sci Protocol Notes PREMISE: Precise pollen collection methods are necessary for crop breeding, but anemophilous pollen is notoriously difficult to capture and control. Here we compared a variety of methods for the controlled capture of cannabis pollen, intended to ease the process of cross‐fertilization for breeding this wind‐pollinated plant, and measured the utility of light spectroscopy for quantifying relative pollen yield. METHODS AND RESULTS: In two independent trials, we compared a control method of pollen collection (hand collection) to either vacuum‐, water‐, or bag‐collection methods. We used visible light spectroscopy to quantify relative pollen yield, and validated this approach using microscopic pollen counts. We determined that pollen yield was highest when using hand collection or vacuum collection, but efficiency did not differ significantly among methods. CONCLUSIONS: To maximize yield, pollen should be collected by hand or vacuum, but all collection methods were equally efficient in a relative sense because yield increased with collection time. We also found that light spectroscopy is an accurate and rapid method of quantifying pollen abundance (R (2) = 0.86) in a liquid suspension. John Wiley and Sons Inc. 2020-09-30 /pmc/articles/PMC7526430/ /pubmed/33014633 http://dx.doi.org/10.1002/aps3.11389 Text en © 2020 Wizenberg et al. Applications in Plant Sciences is published by Wiley Periodicals LLC on behalf of the Botanical Society of America This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Protocol Notes
Wizenberg, Sydney B.
Weis, Arthur E.
Campbell, Lesley G.
Comparing methods for controlled capture and quantification of pollen in Cannabis sativa
title Comparing methods for controlled capture and quantification of pollen in Cannabis sativa
title_full Comparing methods for controlled capture and quantification of pollen in Cannabis sativa
title_fullStr Comparing methods for controlled capture and quantification of pollen in Cannabis sativa
title_full_unstemmed Comparing methods for controlled capture and quantification of pollen in Cannabis sativa
title_short Comparing methods for controlled capture and quantification of pollen in Cannabis sativa
title_sort comparing methods for controlled capture and quantification of pollen in cannabis sativa
topic Protocol Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7526430/
https://www.ncbi.nlm.nih.gov/pubmed/33014633
http://dx.doi.org/10.1002/aps3.11389
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