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Immune-triggering effect of the foodborne parasite Kudoa septempunctata through the C-type lectin Mincle in HT29 cells

Kudoa septempunctata is a myxozoan parasite that causes food poisoning in individuals consuming olive flounder. The present study aimed to investigate the currently insufficiently elucidated early molecular mechanisms of inflammatory responses in the intestine owing to parasite ingestion. After Kudo...

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Autores principales: Shin, Ji-Hun, Yang, Jung-Pyo, Seo, Seung-Hwan, Kim, Sang-Gyun, Kim, Eun-Min, Ham, Do-Won, Shin, Eun-Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7526980/
https://www.ncbi.nlm.nih.gov/pubmed/32843128
http://dx.doi.org/10.5483/BMBRep.2020.53.9.079
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author Shin, Ji-Hun
Yang, Jung-Pyo
Seo, Seung-Hwan
Kim, Sang-Gyun
Kim, Eun-Min
Ham, Do-Won
Shin, Eun-Hee
author_facet Shin, Ji-Hun
Yang, Jung-Pyo
Seo, Seung-Hwan
Kim, Sang-Gyun
Kim, Eun-Min
Ham, Do-Won
Shin, Eun-Hee
author_sort Shin, Ji-Hun
collection PubMed
description Kudoa septempunctata is a myxozoan parasite that causes food poisoning in individuals consuming olive flounder. The present study aimed to investigate the currently insufficiently elucidated early molecular mechanisms of inflammatory responses in the intestine owing to parasite ingestion. After Kudoa spores were isolated from olive flounder, HT29 cells were exposed to spores identified to be alive using SYTO-9 and propidium iodide staining or to antigens of Kudoa spores (KsAg). IL-1β, IL-8, TNF-α and NFKB1 expression and NF-κB activation were assessed using real-time PCR, cytokine array and western blotting. The immunofluorescence of FITC-conjugated lectins, results of ligand binding assays using Mincle-Fc and IgG-Fc, CLEC4E expres-sions in response to KsAg stimulation, and Mincle-dependent NF-κB activation were assessed to clarify the early immunetriggering mechanism. Inflammatory cytokines (IL-1β, GM-CSF and TNF-α), chemokines (IL-8, CCL2, CCL5 and CXCL1) and NF-κB activation (pNF-κB/NF-κB) in HT29 cells increased following stimulation by KsAg. The immunofluorescence results of spores and lectins (concanavalin A and wheat germ agglu-tinin) suggested the importance of Mincle in molecular recog-nition between Kudoa spores and intestinal cells. Practically, data for Mincle-Fc and KsAg binding affinity, CLEC4E mRNA expression, Mincle immunofluorescence staining and hMincle-dependent NF-κB activation demonstrated the involvement of Mincle in the early immune-triggering mechanism. The present study newly elucidated that the molecular recognition and immune-triggering mechanism of K. septempunctata are associated with Mincle on human intestinal epithelial cells.
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spelling pubmed-75269802020-10-12 Immune-triggering effect of the foodborne parasite Kudoa septempunctata through the C-type lectin Mincle in HT29 cells Shin, Ji-Hun Yang, Jung-Pyo Seo, Seung-Hwan Kim, Sang-Gyun Kim, Eun-Min Ham, Do-Won Shin, Eun-Hee BMB Rep Article Kudoa septempunctata is a myxozoan parasite that causes food poisoning in individuals consuming olive flounder. The present study aimed to investigate the currently insufficiently elucidated early molecular mechanisms of inflammatory responses in the intestine owing to parasite ingestion. After Kudoa spores were isolated from olive flounder, HT29 cells were exposed to spores identified to be alive using SYTO-9 and propidium iodide staining or to antigens of Kudoa spores (KsAg). IL-1β, IL-8, TNF-α and NFKB1 expression and NF-κB activation were assessed using real-time PCR, cytokine array and western blotting. The immunofluorescence of FITC-conjugated lectins, results of ligand binding assays using Mincle-Fc and IgG-Fc, CLEC4E expres-sions in response to KsAg stimulation, and Mincle-dependent NF-κB activation were assessed to clarify the early immunetriggering mechanism. Inflammatory cytokines (IL-1β, GM-CSF and TNF-α), chemokines (IL-8, CCL2, CCL5 and CXCL1) and NF-κB activation (pNF-κB/NF-κB) in HT29 cells increased following stimulation by KsAg. The immunofluorescence results of spores and lectins (concanavalin A and wheat germ agglu-tinin) suggested the importance of Mincle in molecular recog-nition between Kudoa spores and intestinal cells. Practically, data for Mincle-Fc and KsAg binding affinity, CLEC4E mRNA expression, Mincle immunofluorescence staining and hMincle-dependent NF-κB activation demonstrated the involvement of Mincle in the early immune-triggering mechanism. The present study newly elucidated that the molecular recognition and immune-triggering mechanism of K. septempunctata are associated with Mincle on human intestinal epithelial cells. Korean Society for Biochemistry and Molecular Biology 2020-09-30 2020-09-30 /pmc/articles/PMC7526980/ /pubmed/32843128 http://dx.doi.org/10.5483/BMBRep.2020.53.9.079 Text en Copyright © 2020 by the The Korean Society for Biochemistry and Molecular Biology This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Shin, Ji-Hun
Yang, Jung-Pyo
Seo, Seung-Hwan
Kim, Sang-Gyun
Kim, Eun-Min
Ham, Do-Won
Shin, Eun-Hee
Immune-triggering effect of the foodborne parasite Kudoa septempunctata through the C-type lectin Mincle in HT29 cells
title Immune-triggering effect of the foodborne parasite Kudoa septempunctata through the C-type lectin Mincle in HT29 cells
title_full Immune-triggering effect of the foodborne parasite Kudoa septempunctata through the C-type lectin Mincle in HT29 cells
title_fullStr Immune-triggering effect of the foodborne parasite Kudoa septempunctata through the C-type lectin Mincle in HT29 cells
title_full_unstemmed Immune-triggering effect of the foodborne parasite Kudoa septempunctata through the C-type lectin Mincle in HT29 cells
title_short Immune-triggering effect of the foodborne parasite Kudoa septempunctata through the C-type lectin Mincle in HT29 cells
title_sort immune-triggering effect of the foodborne parasite kudoa septempunctata through the c-type lectin mincle in ht29 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7526980/
https://www.ncbi.nlm.nih.gov/pubmed/32843128
http://dx.doi.org/10.5483/BMBRep.2020.53.9.079
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