Selective monitoring of insulin secretion after CRISPR interference in intact pancreatic islets despite submaximal infection

Virus-mediated gene knockdown in intact pancreatic islets is technically challenging due to poor infection of the center of the islet. Because the cells that do not have knockdown have normal insulin secretion, measuring changes in insulin secretion after gene knockdown is challenging. We describe a...

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Detalles Bibliográficos
Autores principales: Shariati, Kaavian, Pappalardo, Zachary, Chopra, Deeksha G., Yiv, Nicholas, Sheen, Robin, Ku, Gregory
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527018/
https://www.ncbi.nlm.nih.gov/pubmed/32579048
http://dx.doi.org/10.1080/19382014.2020.1752072

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527018/
https://www.ncbi.nlm.nih.gov/pubmed/32579048
http://dx.doi.org/10.1080/19382014.2020.1752072

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