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Evaluation of low-intensity pulsed ultrasound on doxorubicin delivery in 2D and 3D cancer cell cultures

The aim of our study was to evaluate the influence of low-intensity pulsed US on the delivery of doxorubicin (DOX) into MDA-MB-231 triple-negative breast cancer and A549 non-small cell lung cancer cell 2D and 3D cultures. US with pulse repetition frequency of 10 Hz and 1 MHz center frequency was gen...

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Autores principales: Paškevičiūtė, Miglė, Januškevičienė, Indrė, Sakalauskienė, Kristina, Raišutis, Renaldas, Petrikaitė, Vilma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527335/
https://www.ncbi.nlm.nih.gov/pubmed/32999381
http://dx.doi.org/10.1038/s41598-020-73204-y
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author Paškevičiūtė, Miglė
Januškevičienė, Indrė
Sakalauskienė, Kristina
Raišutis, Renaldas
Petrikaitė, Vilma
author_facet Paškevičiūtė, Miglė
Januškevičienė, Indrė
Sakalauskienė, Kristina
Raišutis, Renaldas
Petrikaitė, Vilma
author_sort Paškevičiūtė, Miglė
collection PubMed
description The aim of our study was to evaluate the influence of low-intensity pulsed US on the delivery of doxorubicin (DOX) into MDA-MB-231 triple-negative breast cancer and A549 non-small cell lung cancer cell 2D and 3D cultures. US with pulse repetition frequency of 10 Hz and 1 MHz center frequency was generated with peak negative pressure of 0.5 MPa and 50% duty cycle. SonoVue microbubbles were used. Spheroids were formed using 3D Bioprinting method. DOX delivery in 2D and 3D cultures was assessed using fluorescence microscopy. US without the addition of microbubbles did not enhance the penetration of DOX into monolayer-cultured cells and tumor spheroids. In the presence of microbubbles US improved the delivery of DOX into the edge end middle zones of A549 and MDA-MB-231 spheroids. Application of low-intensity pulsed US in combination with microbubbles may be a promising approach to enhance the delivery of DOX into tumor spheroids.
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spelling pubmed-75273352020-10-01 Evaluation of low-intensity pulsed ultrasound on doxorubicin delivery in 2D and 3D cancer cell cultures Paškevičiūtė, Miglė Januškevičienė, Indrė Sakalauskienė, Kristina Raišutis, Renaldas Petrikaitė, Vilma Sci Rep Article The aim of our study was to evaluate the influence of low-intensity pulsed US on the delivery of doxorubicin (DOX) into MDA-MB-231 triple-negative breast cancer and A549 non-small cell lung cancer cell 2D and 3D cultures. US with pulse repetition frequency of 10 Hz and 1 MHz center frequency was generated with peak negative pressure of 0.5 MPa and 50% duty cycle. SonoVue microbubbles were used. Spheroids were formed using 3D Bioprinting method. DOX delivery in 2D and 3D cultures was assessed using fluorescence microscopy. US without the addition of microbubbles did not enhance the penetration of DOX into monolayer-cultured cells and tumor spheroids. In the presence of microbubbles US improved the delivery of DOX into the edge end middle zones of A549 and MDA-MB-231 spheroids. Application of low-intensity pulsed US in combination with microbubbles may be a promising approach to enhance the delivery of DOX into tumor spheroids. Nature Publishing Group UK 2020-09-30 /pmc/articles/PMC7527335/ /pubmed/32999381 http://dx.doi.org/10.1038/s41598-020-73204-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Paškevičiūtė, Miglė
Januškevičienė, Indrė
Sakalauskienė, Kristina
Raišutis, Renaldas
Petrikaitė, Vilma
Evaluation of low-intensity pulsed ultrasound on doxorubicin delivery in 2D and 3D cancer cell cultures
title Evaluation of low-intensity pulsed ultrasound on doxorubicin delivery in 2D and 3D cancer cell cultures
title_full Evaluation of low-intensity pulsed ultrasound on doxorubicin delivery in 2D and 3D cancer cell cultures
title_fullStr Evaluation of low-intensity pulsed ultrasound on doxorubicin delivery in 2D and 3D cancer cell cultures
title_full_unstemmed Evaluation of low-intensity pulsed ultrasound on doxorubicin delivery in 2D and 3D cancer cell cultures
title_short Evaluation of low-intensity pulsed ultrasound on doxorubicin delivery in 2D and 3D cancer cell cultures
title_sort evaluation of low-intensity pulsed ultrasound on doxorubicin delivery in 2d and 3d cancer cell cultures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527335/
https://www.ncbi.nlm.nih.gov/pubmed/32999381
http://dx.doi.org/10.1038/s41598-020-73204-y
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