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Effects of Peptide C(12)-OOWW-NH(2) on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum
In this study, the transcriptional profiling of Penicillium digitatum after C(12)O(3)TR treatment was analyzed by RNA-Seq technology. A total of 2562 and 667 genes in P. digitatum were differentially expressed after 2 and 12 h treatment, respectively. These genes were respectively mapped to 91 and 7...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527529/ https://www.ncbi.nlm.nih.gov/pubmed/33042086 http://dx.doi.org/10.3389/fmicb.2020.574882 |
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author | Li, Xindan Feng, Guirong Wang, Wenjun Yi, Lanhua Deng, Lili Zeng, Kaifang |
author_facet | Li, Xindan Feng, Guirong Wang, Wenjun Yi, Lanhua Deng, Lili Zeng, Kaifang |
author_sort | Li, Xindan |
collection | PubMed |
description | In this study, the transcriptional profiling of Penicillium digitatum after C(12)O(3)TR treatment was analyzed by RNA-Seq technology. A total of 2562 and 667 genes in P. digitatum were differentially expressed after 2 and 12 h treatment, respectively. These genes were respectively mapped to 91 and 79 KEGG pathways. The expression patterns of differentially expressed genes (DEGs) at 2 and 12 h were similar, mainly were the metabolic processes in cell wall, cell membrane, genetic information and energy. Particularly, the main metabolic process which was affected by C(12)O(3)TR stress for 2 and 12 h was cell integrity, including cell wall and cell membrane. The changes of chitin in cell wall was observed by Calcofluor White (CFW) staining assay. The weaker blue fluorescence in the cell wall septa, the decrease of β-1, 3-glucan synthase activity and the increase of chitinase and AKP activity showed that C(12)O(3)TR could damage the cell wall integrity. In conclusion, these results suggested that C(12)O(3)TR could inhibit the growth of P. digitatum through various mechanisms at transcriptional level, and could influence the cell wall permeability and integrity. |
format | Online Article Text |
id | pubmed-7527529 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75275292020-10-09 Effects of Peptide C(12)-OOWW-NH(2) on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum Li, Xindan Feng, Guirong Wang, Wenjun Yi, Lanhua Deng, Lili Zeng, Kaifang Front Microbiol Microbiology In this study, the transcriptional profiling of Penicillium digitatum after C(12)O(3)TR treatment was analyzed by RNA-Seq technology. A total of 2562 and 667 genes in P. digitatum were differentially expressed after 2 and 12 h treatment, respectively. These genes were respectively mapped to 91 and 79 KEGG pathways. The expression patterns of differentially expressed genes (DEGs) at 2 and 12 h were similar, mainly were the metabolic processes in cell wall, cell membrane, genetic information and energy. Particularly, the main metabolic process which was affected by C(12)O(3)TR stress for 2 and 12 h was cell integrity, including cell wall and cell membrane. The changes of chitin in cell wall was observed by Calcofluor White (CFW) staining assay. The weaker blue fluorescence in the cell wall septa, the decrease of β-1, 3-glucan synthase activity and the increase of chitinase and AKP activity showed that C(12)O(3)TR could damage the cell wall integrity. In conclusion, these results suggested that C(12)O(3)TR could inhibit the growth of P. digitatum through various mechanisms at transcriptional level, and could influence the cell wall permeability and integrity. Frontiers Media S.A. 2020-09-17 /pmc/articles/PMC7527529/ /pubmed/33042086 http://dx.doi.org/10.3389/fmicb.2020.574882 Text en Copyright © 2020 Li, Feng, Wang, Yi, Deng and Zeng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Li, Xindan Feng, Guirong Wang, Wenjun Yi, Lanhua Deng, Lili Zeng, Kaifang Effects of Peptide C(12)-OOWW-NH(2) on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum |
title | Effects of Peptide C(12)-OOWW-NH(2) on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum |
title_full | Effects of Peptide C(12)-OOWW-NH(2) on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum |
title_fullStr | Effects of Peptide C(12)-OOWW-NH(2) on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum |
title_full_unstemmed | Effects of Peptide C(12)-OOWW-NH(2) on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum |
title_short | Effects of Peptide C(12)-OOWW-NH(2) on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum |
title_sort | effects of peptide c(12)-ooww-nh(2) on transcriptome and cell wall of the postharvest fungal pathogen penicillium digitatum |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527529/ https://www.ncbi.nlm.nih.gov/pubmed/33042086 http://dx.doi.org/10.3389/fmicb.2020.574882 |
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