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Human Cytomegalovirus Genomes Survive Mitosis via the IE19 Chromatin-Tethering Domain
The genomes of DNA tumor viruses regain nuclear localization after nuclear envelope breakdown during mitosis through the action of a viral protein with a chromatin-tethering domain (CTD). Here, we report that the human cytomegalovirus (HCMV) genome is maintained during mitosis by the CTD of the vira...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527735/ https://www.ncbi.nlm.nih.gov/pubmed/32994332 http://dx.doi.org/10.1128/mBio.02410-20 |
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author | Lyon, Shelby M. Yetming, Kristen D. Paulus, Christina Nevels, Michael Kalejta, Robert F. |
author_facet | Lyon, Shelby M. Yetming, Kristen D. Paulus, Christina Nevels, Michael Kalejta, Robert F. |
author_sort | Lyon, Shelby M. |
collection | PubMed |
description | The genomes of DNA tumor viruses regain nuclear localization after nuclear envelope breakdown during mitosis through the action of a viral protein with a chromatin-tethering domain (CTD). Here, we report that the human cytomegalovirus (HCMV) genome is maintained during mitosis by the CTD of the viral IE19 protein. Deletion of the IE19 CTD or disruption of the IE19 splice acceptor site reduced viral genome maintenance and progeny virion formation during infection of dividing fibroblasts, both of which were rescued by IE19 ectopic expression. The discovery of a viral genome maintenance factor during productive infection provides new insight into the mode of HCMV infection implicated in birth defects, organ transplant failure, and cancer. |
format | Online Article Text |
id | pubmed-7527735 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-75277352020-10-19 Human Cytomegalovirus Genomes Survive Mitosis via the IE19 Chromatin-Tethering Domain Lyon, Shelby M. Yetming, Kristen D. Paulus, Christina Nevels, Michael Kalejta, Robert F. mBio Research Article The genomes of DNA tumor viruses regain nuclear localization after nuclear envelope breakdown during mitosis through the action of a viral protein with a chromatin-tethering domain (CTD). Here, we report that the human cytomegalovirus (HCMV) genome is maintained during mitosis by the CTD of the viral IE19 protein. Deletion of the IE19 CTD or disruption of the IE19 splice acceptor site reduced viral genome maintenance and progeny virion formation during infection of dividing fibroblasts, both of which were rescued by IE19 ectopic expression. The discovery of a viral genome maintenance factor during productive infection provides new insight into the mode of HCMV infection implicated in birth defects, organ transplant failure, and cancer. American Society for Microbiology 2020-09-29 /pmc/articles/PMC7527735/ /pubmed/32994332 http://dx.doi.org/10.1128/mBio.02410-20 Text en Copyright © 2020 Lyon et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Lyon, Shelby M. Yetming, Kristen D. Paulus, Christina Nevels, Michael Kalejta, Robert F. Human Cytomegalovirus Genomes Survive Mitosis via the IE19 Chromatin-Tethering Domain |
title | Human Cytomegalovirus Genomes Survive Mitosis via the IE19 Chromatin-Tethering Domain |
title_full | Human Cytomegalovirus Genomes Survive Mitosis via the IE19 Chromatin-Tethering Domain |
title_fullStr | Human Cytomegalovirus Genomes Survive Mitosis via the IE19 Chromatin-Tethering Domain |
title_full_unstemmed | Human Cytomegalovirus Genomes Survive Mitosis via the IE19 Chromatin-Tethering Domain |
title_short | Human Cytomegalovirus Genomes Survive Mitosis via the IE19 Chromatin-Tethering Domain |
title_sort | human cytomegalovirus genomes survive mitosis via the ie19 chromatin-tethering domain |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527735/ https://www.ncbi.nlm.nih.gov/pubmed/32994332 http://dx.doi.org/10.1128/mBio.02410-20 |
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