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Enrichment of beneficial cucumber rhizosphere microbes mediated by organic acid secretion
Resistant cultivars have played important roles in controlling Fusarium wilt disease, but the roles of rhizosphere interactions among different levels of resistant cultivars are still unknown. Here, two phenotypes of cucumber, one resistant and one with increased susceptibility to Fusarium oxysporum...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527982/ https://www.ncbi.nlm.nih.gov/pubmed/33082961 http://dx.doi.org/10.1038/s41438-020-00380-3 |
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author | Wen, Tao Yuan, Jun He, Xiaoming Lin, Yue Huang, Qiwei Shen, Qirong |
author_facet | Wen, Tao Yuan, Jun He, Xiaoming Lin, Yue Huang, Qiwei Shen, Qirong |
author_sort | Wen, Tao |
collection | PubMed |
description | Resistant cultivars have played important roles in controlling Fusarium wilt disease, but the roles of rhizosphere interactions among different levels of resistant cultivars are still unknown. Here, two phenotypes of cucumber, one resistant and one with increased susceptibility to Fusarium oxysporum f.sp. cucumerinum (Foc), were grown in the soil and hydroponically, and then 16S rRNA gene sequencing and nontargeted metabolomics techniques were used to investigate rhizosphere microflora and root exudate profiles. Relatively high microbial community evenness for the Foc-susceptible cultivar was detected, and the relative abundances of Comamonadaceae and Xanthomonadaceae were higher for the Foc-susceptible cultivar than for the other cultivar. FishTaco analysis revealed that specific functional traits, such as protein synthesis and secretion, bacterial chemotaxis, and small organic acid metabolism pathways, were significantly upregulated in the rhizobacterial community of the Foc-susceptible cultivar. A machine-learning approach in conjunction with FishTaco plus metabolic pathway analysis revealed that four organic acids (citric acid, pyruvate acid, succinic acid, and fumarate) were released at higher abundance by the Foc-susceptible cultivar compared with the resistant cultivar, which may be responsible for the recruitment of Comamonadaceae, a potential beneficial microbial group. Further validation demonstrated that Comamonadaceae can be “cultured” by these organic acids. Together, compared with the resistant cultivar, the susceptible cucumber tends to assemble beneficial microbes by secreting more organic acids. |
format | Online Article Text |
id | pubmed-7527982 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-75279822020-10-19 Enrichment of beneficial cucumber rhizosphere microbes mediated by organic acid secretion Wen, Tao Yuan, Jun He, Xiaoming Lin, Yue Huang, Qiwei Shen, Qirong Hortic Res Article Resistant cultivars have played important roles in controlling Fusarium wilt disease, but the roles of rhizosphere interactions among different levels of resistant cultivars are still unknown. Here, two phenotypes of cucumber, one resistant and one with increased susceptibility to Fusarium oxysporum f.sp. cucumerinum (Foc), were grown in the soil and hydroponically, and then 16S rRNA gene sequencing and nontargeted metabolomics techniques were used to investigate rhizosphere microflora and root exudate profiles. Relatively high microbial community evenness for the Foc-susceptible cultivar was detected, and the relative abundances of Comamonadaceae and Xanthomonadaceae were higher for the Foc-susceptible cultivar than for the other cultivar. FishTaco analysis revealed that specific functional traits, such as protein synthesis and secretion, bacterial chemotaxis, and small organic acid metabolism pathways, were significantly upregulated in the rhizobacterial community of the Foc-susceptible cultivar. A machine-learning approach in conjunction with FishTaco plus metabolic pathway analysis revealed that four organic acids (citric acid, pyruvate acid, succinic acid, and fumarate) were released at higher abundance by the Foc-susceptible cultivar compared with the resistant cultivar, which may be responsible for the recruitment of Comamonadaceae, a potential beneficial microbial group. Further validation demonstrated that Comamonadaceae can be “cultured” by these organic acids. Together, compared with the resistant cultivar, the susceptible cucumber tends to assemble beneficial microbes by secreting more organic acids. Nature Publishing Group UK 2020-10-01 /pmc/articles/PMC7527982/ /pubmed/33082961 http://dx.doi.org/10.1038/s41438-020-00380-3 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Wen, Tao Yuan, Jun He, Xiaoming Lin, Yue Huang, Qiwei Shen, Qirong Enrichment of beneficial cucumber rhizosphere microbes mediated by organic acid secretion |
title | Enrichment of beneficial cucumber rhizosphere microbes mediated by organic acid secretion |
title_full | Enrichment of beneficial cucumber rhizosphere microbes mediated by organic acid secretion |
title_fullStr | Enrichment of beneficial cucumber rhizosphere microbes mediated by organic acid secretion |
title_full_unstemmed | Enrichment of beneficial cucumber rhizosphere microbes mediated by organic acid secretion |
title_short | Enrichment of beneficial cucumber rhizosphere microbes mediated by organic acid secretion |
title_sort | enrichment of beneficial cucumber rhizosphere microbes mediated by organic acid secretion |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527982/ https://www.ncbi.nlm.nih.gov/pubmed/33082961 http://dx.doi.org/10.1038/s41438-020-00380-3 |
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