Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy

SARS-CoV-2 is the cause of the ongoing COVID-19 pandemic. Here, we investigated the interaction of this new coronavirus with Vero cells using high resolution scanning electron microscopy. Surface morphology, the interior of infected cells and the distribution of viral particles in both environments...

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Autores principales: Caldas, Lucio Ayres, Carneiro, Fabiana Avila, Higa, Luiza Mendonça, Monteiro, Fábio Luiz, da Silva, Gustavo Peixoto, da Costa, Luciana Jesus, Durigon, Edison Luiz, Tanuri, Amilcar, de Souza, Wanderley
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528159/
https://www.ncbi.nlm.nih.gov/pubmed/32999356
http://dx.doi.org/10.1038/s41598-020-73162-5
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author Caldas, Lucio Ayres
Carneiro, Fabiana Avila
Higa, Luiza Mendonça
Monteiro, Fábio Luiz
da Silva, Gustavo Peixoto
da Costa, Luciana Jesus
Durigon, Edison Luiz
Tanuri, Amilcar
de Souza, Wanderley
author_facet Caldas, Lucio Ayres
Carneiro, Fabiana Avila
Higa, Luiza Mendonça
Monteiro, Fábio Luiz
da Silva, Gustavo Peixoto
da Costa, Luciana Jesus
Durigon, Edison Luiz
Tanuri, Amilcar
de Souza, Wanderley
author_sort Caldas, Lucio Ayres
collection PubMed
description SARS-CoV-2 is the cause of the ongoing COVID-19 pandemic. Here, we investigated the interaction of this new coronavirus with Vero cells using high resolution scanning electron microscopy. Surface morphology, the interior of infected cells and the distribution of viral particles in both environments were observed 2 and 48 h after infection. We showed areas of viral processing, details of vacuole contents, and viral interactions with the cell surface. Intercellular connections were also approached, and viral particles were adhered to these extensions suggesting direct cell-to-cell transmission of SARS-CoV-2.
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spelling pubmed-75281592020-10-02 Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy Caldas, Lucio Ayres Carneiro, Fabiana Avila Higa, Luiza Mendonça Monteiro, Fábio Luiz da Silva, Gustavo Peixoto da Costa, Luciana Jesus Durigon, Edison Luiz Tanuri, Amilcar de Souza, Wanderley Sci Rep Article SARS-CoV-2 is the cause of the ongoing COVID-19 pandemic. Here, we investigated the interaction of this new coronavirus with Vero cells using high resolution scanning electron microscopy. Surface morphology, the interior of infected cells and the distribution of viral particles in both environments were observed 2 and 48 h after infection. We showed areas of viral processing, details of vacuole contents, and viral interactions with the cell surface. Intercellular connections were also approached, and viral particles were adhered to these extensions suggesting direct cell-to-cell transmission of SARS-CoV-2. Nature Publishing Group UK 2020-09-30 /pmc/articles/PMC7528159/ /pubmed/32999356 http://dx.doi.org/10.1038/s41598-020-73162-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Caldas, Lucio Ayres
Carneiro, Fabiana Avila
Higa, Luiza Mendonça
Monteiro, Fábio Luiz
da Silva, Gustavo Peixoto
da Costa, Luciana Jesus
Durigon, Edison Luiz
Tanuri, Amilcar
de Souza, Wanderley
Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy
title Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy
title_full Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy
title_fullStr Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy
title_full_unstemmed Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy
title_short Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy
title_sort ultrastructural analysis of sars-cov-2 interactions with the host cell via high resolution scanning electron microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528159/
https://www.ncbi.nlm.nih.gov/pubmed/32999356
http://dx.doi.org/10.1038/s41598-020-73162-5
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