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G-Quadruplex Helicase DHX36/G4R1 Engages Nuclear Lamina Proteins in Quiescent Breast Cancer Cells

[Image: see text] G-quadruplexes (G4s) are nucleic acid structures found enriched within gene regulatory sequences. G4s control fundamental cellular processes, including replication, transcription, and translation. Proto-oncogenes are enriched with G4 sequences, while tumor-suppressor genes are depl...

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Autores principales: Richardson, Adam. E., Zentz, Zachary. A., Chambers, Antonio E., Sandwith, Siara N., Reisinger, Michael A., Saunders, Destinee W., Tompkins, Joshua D., Riggs, Arthur D., Routh, Eric D., Rubenstein, Eric M., Smaldino, Melissa A., Vaughn, James P., Haney, Robert A., Smaldino, Philip J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528498/
https://www.ncbi.nlm.nih.gov/pubmed/33015511
http://dx.doi.org/10.1021/acsomega.0c03723
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author Richardson, Adam. E.
Zentz, Zachary. A.
Chambers, Antonio E.
Sandwith, Siara N.
Reisinger, Michael A.
Saunders, Destinee W.
Tompkins, Joshua D.
Riggs, Arthur D.
Routh, Eric D.
Rubenstein, Eric M.
Smaldino, Melissa A.
Vaughn, James P.
Haney, Robert A.
Smaldino, Philip J.
author_facet Richardson, Adam. E.
Zentz, Zachary. A.
Chambers, Antonio E.
Sandwith, Siara N.
Reisinger, Michael A.
Saunders, Destinee W.
Tompkins, Joshua D.
Riggs, Arthur D.
Routh, Eric D.
Rubenstein, Eric M.
Smaldino, Melissa A.
Vaughn, James P.
Haney, Robert A.
Smaldino, Philip J.
author_sort Richardson, Adam. E.
collection PubMed
description [Image: see text] G-quadruplexes (G4s) are nucleic acid structures found enriched within gene regulatory sequences. G4s control fundamental cellular processes, including replication, transcription, and translation. Proto-oncogenes are enriched with G4 sequences, while tumor-suppressor genes are depleted, suggesting roles for G4s in cell survival and proliferation. Specialized helicases participate in G4-mediated gene regulation via enzymatic unwinding activity. One such enzyme, DHX36/G4R1, is the major G4-helicase and is a master regulator of G4-DNAs and mRNAs. G4-resolution promotes the expression of proproliferative genes; as such, DHX36/G4R1 promotes cell proliferation. Little is known about how DHX36/G4R1 itself is regulated in nondividing cells. We hypothesized that DHX36/G4R1 protein binding partners are altered when a cell transitions from a dividing to a quiescent state. We found that DHX36/G4R1 co-purifies with a distinct set of proteins under quiescent conditions, which may represent a novel complex that regulates DHX36/G4R1 during cell cycle transitions and have implications for development and cancer.
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spelling pubmed-75284982020-10-02 G-Quadruplex Helicase DHX36/G4R1 Engages Nuclear Lamina Proteins in Quiescent Breast Cancer Cells Richardson, Adam. E. Zentz, Zachary. A. Chambers, Antonio E. Sandwith, Siara N. Reisinger, Michael A. Saunders, Destinee W. Tompkins, Joshua D. Riggs, Arthur D. Routh, Eric D. Rubenstein, Eric M. Smaldino, Melissa A. Vaughn, James P. Haney, Robert A. Smaldino, Philip J. ACS Omega [Image: see text] G-quadruplexes (G4s) are nucleic acid structures found enriched within gene regulatory sequences. G4s control fundamental cellular processes, including replication, transcription, and translation. Proto-oncogenes are enriched with G4 sequences, while tumor-suppressor genes are depleted, suggesting roles for G4s in cell survival and proliferation. Specialized helicases participate in G4-mediated gene regulation via enzymatic unwinding activity. One such enzyme, DHX36/G4R1, is the major G4-helicase and is a master regulator of G4-DNAs and mRNAs. G4-resolution promotes the expression of proproliferative genes; as such, DHX36/G4R1 promotes cell proliferation. Little is known about how DHX36/G4R1 itself is regulated in nondividing cells. We hypothesized that DHX36/G4R1 protein binding partners are altered when a cell transitions from a dividing to a quiescent state. We found that DHX36/G4R1 co-purifies with a distinct set of proteins under quiescent conditions, which may represent a novel complex that regulates DHX36/G4R1 during cell cycle transitions and have implications for development and cancer. American Chemical Society 2020-09-16 /pmc/articles/PMC7528498/ /pubmed/33015511 http://dx.doi.org/10.1021/acsomega.0c03723 Text en This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Richardson, Adam. E.
Zentz, Zachary. A.
Chambers, Antonio E.
Sandwith, Siara N.
Reisinger, Michael A.
Saunders, Destinee W.
Tompkins, Joshua D.
Riggs, Arthur D.
Routh, Eric D.
Rubenstein, Eric M.
Smaldino, Melissa A.
Vaughn, James P.
Haney, Robert A.
Smaldino, Philip J.
G-Quadruplex Helicase DHX36/G4R1 Engages Nuclear Lamina Proteins in Quiescent Breast Cancer Cells
title G-Quadruplex Helicase DHX36/G4R1 Engages Nuclear Lamina Proteins in Quiescent Breast Cancer Cells
title_full G-Quadruplex Helicase DHX36/G4R1 Engages Nuclear Lamina Proteins in Quiescent Breast Cancer Cells
title_fullStr G-Quadruplex Helicase DHX36/G4R1 Engages Nuclear Lamina Proteins in Quiescent Breast Cancer Cells
title_full_unstemmed G-Quadruplex Helicase DHX36/G4R1 Engages Nuclear Lamina Proteins in Quiescent Breast Cancer Cells
title_short G-Quadruplex Helicase DHX36/G4R1 Engages Nuclear Lamina Proteins in Quiescent Breast Cancer Cells
title_sort g-quadruplex helicase dhx36/g4r1 engages nuclear lamina proteins in quiescent breast cancer cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528498/
https://www.ncbi.nlm.nih.gov/pubmed/33015511
http://dx.doi.org/10.1021/acsomega.0c03723
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