Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2

SNAI2/SLUG, a metastasis-promoting transcription factor, is a labile protein that is degraded through the ubiquitin proteasome degradation system. Here, we conducted comprehensive gain- and loss-of-function screens using a human DUB cDNA library of 65 genes and an siRNA library of 98 genes, and iden...

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Detalles Bibliográficos
Autores principales: Li, Wenyang, Shen, Minhong, Jiang, Yi-Zhou, Zhang, Ruina, Zheng, Hanqiu, Wei, Yong, Shao, Zhi-Ming, Kang, Yibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2020
Materias:
Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528704/
https://www.ncbi.nlm.nih.gov/pubmed/32943575
http://dx.doi.org/10.1101/gad.339804.120
Descripción
Sumario:SNAI2/SLUG, a metastasis-promoting transcription factor, is a labile protein that is degraded through the ubiquitin proteasome degradation system. Here, we conducted comprehensive gain- and loss-of-function screens using a human DUB cDNA library of 65 genes and an siRNA library of 98 genes, and identified USP20 as a deubiquitinase (DUB) that regulates SNAI2 ubiquitination and stability. Further investigation of USP20 demonstrated its function in promoting migration, invasion, and metastasis of breast cancer. USP20 positively correlates with SNAI2 protein level in breast tumor samples, and higher USP20 expression is associated with poor prognosis in ER(−) breast cancer patients.

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