Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2

SNAI2/SLUG, a metastasis-promoting transcription factor, is a labile protein that is degraded through the ubiquitin proteasome degradation system. Here, we conducted comprehensive gain- and loss-of-function screens using a human DUB cDNA library of 65 genes and an siRNA library of 98 genes, and iden...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Wenyang, Shen, Minhong, Jiang, Yi-Zhou, Zhang, Ruina, Zheng, Hanqiu, Wei, Yong, Shao, Zhi-Ming, Kang, Yibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2020
Materias:
Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528704/
https://www.ncbi.nlm.nih.gov/pubmed/32943575
http://dx.doi.org/10.1101/gad.339804.120
_version_ 1783589314039382016
author Li, Wenyang
Shen, Minhong
Jiang, Yi-Zhou
Zhang, Ruina
Zheng, Hanqiu
Wei, Yong
Shao, Zhi-Ming
Kang, Yibin
author_facet Li, Wenyang
Shen, Minhong
Jiang, Yi-Zhou
Zhang, Ruina
Zheng, Hanqiu
Wei, Yong
Shao, Zhi-Ming
Kang, Yibin
author_sort Li, Wenyang
collection PubMed
description SNAI2/SLUG, a metastasis-promoting transcription factor, is a labile protein that is degraded through the ubiquitin proteasome degradation system. Here, we conducted comprehensive gain- and loss-of-function screens using a human DUB cDNA library of 65 genes and an siRNA library of 98 genes, and identified USP20 as a deubiquitinase (DUB) that regulates SNAI2 ubiquitination and stability. Further investigation of USP20 demonstrated its function in promoting migration, invasion, and metastasis of breast cancer. USP20 positively correlates with SNAI2 protein level in breast tumor samples, and higher USP20 expression is associated with poor prognosis in ER(−) breast cancer patients.
format Online
Article
Text
id pubmed-7528704
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Cold Spring Harbor Laboratory Press
record_format MEDLINE/PubMed
spelling pubmed-75287042021-04-01 Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2 Li, Wenyang Shen, Minhong Jiang, Yi-Zhou Zhang, Ruina Zheng, Hanqiu Wei, Yong Shao, Zhi-Ming Kang, Yibin Genes Dev Research Communication SNAI2/SLUG, a metastasis-promoting transcription factor, is a labile protein that is degraded through the ubiquitin proteasome degradation system. Here, we conducted comprehensive gain- and loss-of-function screens using a human DUB cDNA library of 65 genes and an siRNA library of 98 genes, and identified USP20 as a deubiquitinase (DUB) that regulates SNAI2 ubiquitination and stability. Further investigation of USP20 demonstrated its function in promoting migration, invasion, and metastasis of breast cancer. USP20 positively correlates with SNAI2 protein level in breast tumor samples, and higher USP20 expression is associated with poor prognosis in ER(−) breast cancer patients. Cold Spring Harbor Laboratory Press 2020-10-01 /pmc/articles/PMC7528704/ /pubmed/32943575 http://dx.doi.org/10.1101/gad.339804.120 Text en © 2020 Li et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Communication
Li, Wenyang
Shen, Minhong
Jiang, Yi-Zhou
Zhang, Ruina
Zheng, Hanqiu
Wei, Yong
Shao, Zhi-Ming
Kang, Yibin
Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2
title Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2
title_full Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2
title_fullStr Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2
title_full_unstemmed Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2
title_short Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2
title_sort deubiquitinase usp20 promotes breast cancer metastasis by stabilizing snai2
topic Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528704/
https://www.ncbi.nlm.nih.gov/pubmed/32943575
http://dx.doi.org/10.1101/gad.339804.120
work_keys_str_mv AT liwenyang deubiquitinaseusp20promotesbreastcancermetastasisbystabilizingsnai2
AT shenminhong deubiquitinaseusp20promotesbreastcancermetastasisbystabilizingsnai2
AT jiangyizhou deubiquitinaseusp20promotesbreastcancermetastasisbystabilizingsnai2
AT zhangruina deubiquitinaseusp20promotesbreastcancermetastasisbystabilizingsnai2
AT zhenghanqiu deubiquitinaseusp20promotesbreastcancermetastasisbystabilizingsnai2
AT weiyong deubiquitinaseusp20promotesbreastcancermetastasisbystabilizingsnai2
AT shaozhiming deubiquitinaseusp20promotesbreastcancermetastasisbystabilizingsnai2
AT kangyibin deubiquitinaseusp20promotesbreastcancermetastasisbystabilizingsnai2

Ejemplares similares