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Molecular detection and characterization of Carnivore chaphamaparvovirus 1 in dogs

Canine chaphamaparvovirus (CaChPV) is a newly recognised parvovirus discovered by metagenomic analysis during an outbreak of diarrhoea in dogs in Colorado, USA, in 2017 and more recently detected in diarrhoeic dogs in China. Whether the virus plays a role as canine pathogen and whether it is distrib...

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Autores principales: Palombieri, Andrea, Di Profio, Federica, Lanave, Gianvito, Capozza, Paolo, Marsilio, Fulvio, Martella, Vito, Di Martino, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528909/
https://www.ncbi.nlm.nih.gov/pubmed/33069035
http://dx.doi.org/10.1016/j.vetmic.2020.108878
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author Palombieri, Andrea
Di Profio, Federica
Lanave, Gianvito
Capozza, Paolo
Marsilio, Fulvio
Martella, Vito
Di Martino, Barbara
author_facet Palombieri, Andrea
Di Profio, Federica
Lanave, Gianvito
Capozza, Paolo
Marsilio, Fulvio
Martella, Vito
Di Martino, Barbara
author_sort Palombieri, Andrea
collection PubMed
description Canine chaphamaparvovirus (CaChPV) is a newly recognised parvovirus discovered by metagenomic analysis during an outbreak of diarrhoea in dogs in Colorado, USA, in 2017 and more recently detected in diarrhoeic dogs in China. Whether the virus plays a role as canine pathogen and whether it is distributed elsewhere, in other geographical areas, is not known. We performed a case-control study to investigate the possible association of CaChPV with enteritis in dogs. CaChPV DNA was detected both in the stools of diarrhoeic dogs (1.9 %, 3/155) and of healthy animals (1.6 %, 2/120). All the CaChPV-infected dogs with diarrhea were mixed infected with other enteric viruses such as canine parvovirus (formerly CPV-2), canine bufavirus (CBuV) and canine coronavirus (CCoV), whilst none of the asymptomatic CaChPV positive animals resulted co-infected. The nearly full-length genome and the partial capsid protein (VP) gene of three canine strains, Te/36OVUD/19/ITA, Te/37OVUD/19/ITA and Te/70OVUD/19/ITA, were reconstructed. Upon phylogenetic analyses based on the NS1 and VP aa sequences, the Italian CaChPV strains tightly clustered with the American reference viruses. Distinctive residues could be mapped to the deduced variable regions of the VP of canine and feline chaphamaparvoviruses, considered as important markers of host range and pathogenicity for parvoviruses.
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spelling pubmed-75289092020-10-02 Molecular detection and characterization of Carnivore chaphamaparvovirus 1 in dogs Palombieri, Andrea Di Profio, Federica Lanave, Gianvito Capozza, Paolo Marsilio, Fulvio Martella, Vito Di Martino, Barbara Vet Microbiol Article Canine chaphamaparvovirus (CaChPV) is a newly recognised parvovirus discovered by metagenomic analysis during an outbreak of diarrhoea in dogs in Colorado, USA, in 2017 and more recently detected in diarrhoeic dogs in China. Whether the virus plays a role as canine pathogen and whether it is distributed elsewhere, in other geographical areas, is not known. We performed a case-control study to investigate the possible association of CaChPV with enteritis in dogs. CaChPV DNA was detected both in the stools of diarrhoeic dogs (1.9 %, 3/155) and of healthy animals (1.6 %, 2/120). All the CaChPV-infected dogs with diarrhea were mixed infected with other enteric viruses such as canine parvovirus (formerly CPV-2), canine bufavirus (CBuV) and canine coronavirus (CCoV), whilst none of the asymptomatic CaChPV positive animals resulted co-infected. The nearly full-length genome and the partial capsid protein (VP) gene of three canine strains, Te/36OVUD/19/ITA, Te/37OVUD/19/ITA and Te/70OVUD/19/ITA, were reconstructed. Upon phylogenetic analyses based on the NS1 and VP aa sequences, the Italian CaChPV strains tightly clustered with the American reference viruses. Distinctive residues could be mapped to the deduced variable regions of the VP of canine and feline chaphamaparvoviruses, considered as important markers of host range and pathogenicity for parvoviruses. Elsevier B.V. 2020-12 2020-10-01 /pmc/articles/PMC7528909/ /pubmed/33069035 http://dx.doi.org/10.1016/j.vetmic.2020.108878 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Palombieri, Andrea
Di Profio, Federica
Lanave, Gianvito
Capozza, Paolo
Marsilio, Fulvio
Martella, Vito
Di Martino, Barbara
Molecular detection and characterization of Carnivore chaphamaparvovirus 1 in dogs
title Molecular detection and characterization of Carnivore chaphamaparvovirus 1 in dogs
title_full Molecular detection and characterization of Carnivore chaphamaparvovirus 1 in dogs
title_fullStr Molecular detection and characterization of Carnivore chaphamaparvovirus 1 in dogs
title_full_unstemmed Molecular detection and characterization of Carnivore chaphamaparvovirus 1 in dogs
title_short Molecular detection and characterization of Carnivore chaphamaparvovirus 1 in dogs
title_sort molecular detection and characterization of carnivore chaphamaparvovirus 1 in dogs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528909/
https://www.ncbi.nlm.nih.gov/pubmed/33069035
http://dx.doi.org/10.1016/j.vetmic.2020.108878
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