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Expression profiles of the SARS-CoV-2 host invasion genes in nasopharyngeal and oropharyngeal swabs of COVID-19 patients

We collect the nasopharyngeal and oropharyngeal swabs of 63 subjects with severe symptoms or contacts with COVID-19 confirmed cases to perform a pilot-study aimed to verify the “in situ” expression of SARS-CoV-2 host invasion genes (ACE2, TMPRSS2, PCSK3, EMILIN1, EMILIN2, MMRN1, MMRN2, DPP4). ACE2 (...

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Autores principales: Amati, Francesca, Vancheri, Chiara, Latini, Andrea, Colona, Vito Luigi, Grelli, Sandro, D'Apice, Maria Rosaria, Balestrieri, Emanuela, Passarelli, Chiara, Minutolo, Antonella, Loddo, Sara, Di Lorenzo, Andrea, Rogliani, Paola, Andreoni, Massimo, Novelli, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528978/
https://www.ncbi.nlm.nih.gov/pubmed/33024851
http://dx.doi.org/10.1016/j.heliyon.2020.e05143
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author Amati, Francesca
Vancheri, Chiara
Latini, Andrea
Colona, Vito Luigi
Grelli, Sandro
D'Apice, Maria Rosaria
Balestrieri, Emanuela
Passarelli, Chiara
Minutolo, Antonella
Loddo, Sara
Di Lorenzo, Andrea
Rogliani, Paola
Andreoni, Massimo
Novelli, Giuseppe
author_facet Amati, Francesca
Vancheri, Chiara
Latini, Andrea
Colona, Vito Luigi
Grelli, Sandro
D'Apice, Maria Rosaria
Balestrieri, Emanuela
Passarelli, Chiara
Minutolo, Antonella
Loddo, Sara
Di Lorenzo, Andrea
Rogliani, Paola
Andreoni, Massimo
Novelli, Giuseppe
author_sort Amati, Francesca
collection PubMed
description We collect the nasopharyngeal and oropharyngeal swabs of 63 subjects with severe symptoms or contacts with COVID-19 confirmed cases to perform a pilot-study aimed to verify the “in situ” expression of SARS-CoV-2 host invasion genes (ACE2, TMPRSS2, PCSK3, EMILIN1, EMILIN2, MMRN1, MMRN2, DPP4). ACE2 (FC = +1.88, p ≤ 0.05) and DPP4 (FC = +3, p < 0.01) genes showed a significant overexpression in COVID-19 patients. ACE2 and DPP4 expression levels had a good performance (AUC = 0.75; p < 0.001) in distinguishing COVID-19 patients from negative subjects. Interestingly, we found a significant positive association of ACE2 mRNA and PCSK3, EMILIN1, MMRN1 and MMRN2 expression and of DPP4 mRNA and EMILIN2 expression only in COVID-19 patients. Noteworthy, a subgroup of severe COVID-19 (n = 7) patients, showed significant high level of ACE2 mRNA and another subgroup of less severe COVID-19 patients (n = 6) significant raised DPP4 levels. These results indicate that a group of SARS-CoV-2 host invasion genes are functionally related in COVID-19 patients and suggests that ACE2 and DPP4 expression level could act as genomic biomarkers. Moreover, at the best of our knowledge, this is the first study that shows an elevated DPP4 expression in naso- and oropharyngeal swabs of COVID-19 patient thus suggesting a functional role of DPP4 in SARS-CoV-2 infections.
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spelling pubmed-75289782020-10-02 Expression profiles of the SARS-CoV-2 host invasion genes in nasopharyngeal and oropharyngeal swabs of COVID-19 patients Amati, Francesca Vancheri, Chiara Latini, Andrea Colona, Vito Luigi Grelli, Sandro D'Apice, Maria Rosaria Balestrieri, Emanuela Passarelli, Chiara Minutolo, Antonella Loddo, Sara Di Lorenzo, Andrea Rogliani, Paola Andreoni, Massimo Novelli, Giuseppe Heliyon Research Article We collect the nasopharyngeal and oropharyngeal swabs of 63 subjects with severe symptoms or contacts with COVID-19 confirmed cases to perform a pilot-study aimed to verify the “in situ” expression of SARS-CoV-2 host invasion genes (ACE2, TMPRSS2, PCSK3, EMILIN1, EMILIN2, MMRN1, MMRN2, DPP4). ACE2 (FC = +1.88, p ≤ 0.05) and DPP4 (FC = +3, p < 0.01) genes showed a significant overexpression in COVID-19 patients. ACE2 and DPP4 expression levels had a good performance (AUC = 0.75; p < 0.001) in distinguishing COVID-19 patients from negative subjects. Interestingly, we found a significant positive association of ACE2 mRNA and PCSK3, EMILIN1, MMRN1 and MMRN2 expression and of DPP4 mRNA and EMILIN2 expression only in COVID-19 patients. Noteworthy, a subgroup of severe COVID-19 (n = 7) patients, showed significant high level of ACE2 mRNA and another subgroup of less severe COVID-19 patients (n = 6) significant raised DPP4 levels. These results indicate that a group of SARS-CoV-2 host invasion genes are functionally related in COVID-19 patients and suggests that ACE2 and DPP4 expression level could act as genomic biomarkers. Moreover, at the best of our knowledge, this is the first study that shows an elevated DPP4 expression in naso- and oropharyngeal swabs of COVID-19 patient thus suggesting a functional role of DPP4 in SARS-CoV-2 infections. Elsevier 2020-10-01 /pmc/articles/PMC7528978/ /pubmed/33024851 http://dx.doi.org/10.1016/j.heliyon.2020.e05143 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Amati, Francesca
Vancheri, Chiara
Latini, Andrea
Colona, Vito Luigi
Grelli, Sandro
D'Apice, Maria Rosaria
Balestrieri, Emanuela
Passarelli, Chiara
Minutolo, Antonella
Loddo, Sara
Di Lorenzo, Andrea
Rogliani, Paola
Andreoni, Massimo
Novelli, Giuseppe
Expression profiles of the SARS-CoV-2 host invasion genes in nasopharyngeal and oropharyngeal swabs of COVID-19 patients
title Expression profiles of the SARS-CoV-2 host invasion genes in nasopharyngeal and oropharyngeal swabs of COVID-19 patients
title_full Expression profiles of the SARS-CoV-2 host invasion genes in nasopharyngeal and oropharyngeal swabs of COVID-19 patients
title_fullStr Expression profiles of the SARS-CoV-2 host invasion genes in nasopharyngeal and oropharyngeal swabs of COVID-19 patients
title_full_unstemmed Expression profiles of the SARS-CoV-2 host invasion genes in nasopharyngeal and oropharyngeal swabs of COVID-19 patients
title_short Expression profiles of the SARS-CoV-2 host invasion genes in nasopharyngeal and oropharyngeal swabs of COVID-19 patients
title_sort expression profiles of the sars-cov-2 host invasion genes in nasopharyngeal and oropharyngeal swabs of covid-19 patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528978/
https://www.ncbi.nlm.nih.gov/pubmed/33024851
http://dx.doi.org/10.1016/j.heliyon.2020.e05143
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