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Electronic cigarettes cause alteration in cardiac structure and function in diet-induced obese mice
In spite of the widespread use of electronic cigarettes, also known as e-cigarettes, and the proposed adverse cardiac effects of nicotine, the detrimental effects of e-cigarettes on the heart are not well known. This study examines the detrimental effects of e-cigarettes with nicotine at doses that...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529198/ https://www.ncbi.nlm.nih.gov/pubmed/33002059 http://dx.doi.org/10.1371/journal.pone.0239671 |
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author | Hasan, Kamrul M. Friedman, Theodore C. Parveen, Meher Espinoza-Derout, Jorge Bautista, Francisco Razipour, Mohammad M. Shao, Xuesi M. Jordan, Maria C. Roos, Kenneth P. Mahata, Sushil K. Sinha-Hikim, Amiya P. |
author_facet | Hasan, Kamrul M. Friedman, Theodore C. Parveen, Meher Espinoza-Derout, Jorge Bautista, Francisco Razipour, Mohammad M. Shao, Xuesi M. Jordan, Maria C. Roos, Kenneth P. Mahata, Sushil K. Sinha-Hikim, Amiya P. |
author_sort | Hasan, Kamrul M. |
collection | PubMed |
description | In spite of the widespread use of electronic cigarettes, also known as e-cigarettes, and the proposed adverse cardiac effects of nicotine, the detrimental effects of e-cigarettes on the heart are not well known. This study examines the detrimental effects of e-cigarettes with nicotine at doses that yield circulating nicotine and cotinine in the ranges similar to the levels found in habitual smokers, and a high fat diet (HFD) on cardiac structure and function in a commonly used model of diet-induced obesity (DIO). C57BL/6J mice on an HFD were exposed to e-cigarette in the presence (2.4% nicotine) or absence (0% nicotine) of nicotine and saline aerosol for 12 weeks. Echocardiographic data demonstrated a decrease in left ventricular (LV) fractional shortening, LV ejection fraction, and velocity of circumferential fiber shortening (VCF) in mice treated with e-cigarette (2.4% nicotine) compared to e-cigarette (0% nicotine) or saline exposed mice. Cardiomyocytes (CMs) of mice treated with e-cigarette (2.4% nicotine) exhibited LV abnormalities, including lipid accumulation (ventricular steatosis), myofibrillar derangement and destruction, and mitochondrial hypertrophy, as revealed by transmission electron microscopy. The detrimental effects of e-cigarettes (2.4% nicotine) on cardiac structure and function was accompanied by increased oxidative stress, plasma free fatty acid levels, CM apoptosis, and inactivation of AMP-activated protein kinase and activation of its downstream target, acetyl-CoA-carboxylase. Our results indicate profound adverse effects of e-cigarettes (2.4% nicotine) on the heart in obese mice and raise questions about the safety of the nicotine e-cigarettes use. |
format | Online Article Text |
id | pubmed-7529198 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-75291982020-10-02 Electronic cigarettes cause alteration in cardiac structure and function in diet-induced obese mice Hasan, Kamrul M. Friedman, Theodore C. Parveen, Meher Espinoza-Derout, Jorge Bautista, Francisco Razipour, Mohammad M. Shao, Xuesi M. Jordan, Maria C. Roos, Kenneth P. Mahata, Sushil K. Sinha-Hikim, Amiya P. PLoS One Research Article In spite of the widespread use of electronic cigarettes, also known as e-cigarettes, and the proposed adverse cardiac effects of nicotine, the detrimental effects of e-cigarettes on the heart are not well known. This study examines the detrimental effects of e-cigarettes with nicotine at doses that yield circulating nicotine and cotinine in the ranges similar to the levels found in habitual smokers, and a high fat diet (HFD) on cardiac structure and function in a commonly used model of diet-induced obesity (DIO). C57BL/6J mice on an HFD were exposed to e-cigarette in the presence (2.4% nicotine) or absence (0% nicotine) of nicotine and saline aerosol for 12 weeks. Echocardiographic data demonstrated a decrease in left ventricular (LV) fractional shortening, LV ejection fraction, and velocity of circumferential fiber shortening (VCF) in mice treated with e-cigarette (2.4% nicotine) compared to e-cigarette (0% nicotine) or saline exposed mice. Cardiomyocytes (CMs) of mice treated with e-cigarette (2.4% nicotine) exhibited LV abnormalities, including lipid accumulation (ventricular steatosis), myofibrillar derangement and destruction, and mitochondrial hypertrophy, as revealed by transmission electron microscopy. The detrimental effects of e-cigarettes (2.4% nicotine) on cardiac structure and function was accompanied by increased oxidative stress, plasma free fatty acid levels, CM apoptosis, and inactivation of AMP-activated protein kinase and activation of its downstream target, acetyl-CoA-carboxylase. Our results indicate profound adverse effects of e-cigarettes (2.4% nicotine) on the heart in obese mice and raise questions about the safety of the nicotine e-cigarettes use. Public Library of Science 2020-10-01 /pmc/articles/PMC7529198/ /pubmed/33002059 http://dx.doi.org/10.1371/journal.pone.0239671 Text en © 2020 Hasan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Hasan, Kamrul M. Friedman, Theodore C. Parveen, Meher Espinoza-Derout, Jorge Bautista, Francisco Razipour, Mohammad M. Shao, Xuesi M. Jordan, Maria C. Roos, Kenneth P. Mahata, Sushil K. Sinha-Hikim, Amiya P. Electronic cigarettes cause alteration in cardiac structure and function in diet-induced obese mice |
title | Electronic cigarettes cause alteration in cardiac structure and function in diet-induced obese mice |
title_full | Electronic cigarettes cause alteration in cardiac structure and function in diet-induced obese mice |
title_fullStr | Electronic cigarettes cause alteration in cardiac structure and function in diet-induced obese mice |
title_full_unstemmed | Electronic cigarettes cause alteration in cardiac structure and function in diet-induced obese mice |
title_short | Electronic cigarettes cause alteration in cardiac structure and function in diet-induced obese mice |
title_sort | electronic cigarettes cause alteration in cardiac structure and function in diet-induced obese mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529198/ https://www.ncbi.nlm.nih.gov/pubmed/33002059 http://dx.doi.org/10.1371/journal.pone.0239671 |
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