Cargando…

Collocation of avian and mammal antibodies to develop a rapid and sensitive diagnostic tool for Russell's Vipers Snakebite

Russell’s vipers (RVs) envenoming is an important public health issue in South-East Asia. Disseminated intravascular coagulopathy, systemic bleeding, hemolysis, and acute renal injury are obvious problems that develop in most cases, and neuromuscular junction blocks are an additional problem caused...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Jing-Hua, Lo, Che-Min, Chuang, Ssu-Han, Chiang, Chao-Hung, Wang, Sheng-Der, Lin, Tsung-Yi, Liao, Jiunn-Wang, Hung, Dong-Zong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529284/
https://www.ncbi.nlm.nih.gov/pubmed/32956365
http://dx.doi.org/10.1371/journal.pntd.0008701
_version_ 1783589405828579328
author Lin, Jing-Hua
Lo, Che-Min
Chuang, Ssu-Han
Chiang, Chao-Hung
Wang, Sheng-Der
Lin, Tsung-Yi
Liao, Jiunn-Wang
Hung, Dong-Zong
author_facet Lin, Jing-Hua
Lo, Che-Min
Chuang, Ssu-Han
Chiang, Chao-Hung
Wang, Sheng-Der
Lin, Tsung-Yi
Liao, Jiunn-Wang
Hung, Dong-Zong
author_sort Lin, Jing-Hua
collection PubMed
description Russell’s vipers (RVs) envenoming is an important public health issue in South-East Asia. Disseminated intravascular coagulopathy, systemic bleeding, hemolysis, and acute renal injury are obvious problems that develop in most cases, and neuromuscular junction blocks are an additional problem caused by western RV snakebite. The complex presentations usually are an obstacle to early diagnosis and antivenom administration. Here, we tried to produce highly specific antibodies in goose yolks for use in a paper-based microfluidic diagnostic kit, immunochromatographic test of viper (ICT-Viper), to distinguish RVs from other vipers and even cobra snakebite in Asia. We used indirect ELISA to monitor specific goose IgY production and western blotting to illustrate the interaction of avian or mammal antibody with venom proteins. The ICT-Viper was tested not only in prepared samples but also in stored patient serum to demonstrate its preliminary efficacy. The results revealed that specific anti-Daboia russelii IgY could be raised in goose eggs effectively without inducing adverse effects. When it was collocated with horse anti-Daboia siamensis antibody, which broadly reacted with most of the venom proteins of both types of Russell’s viper, the false cross-reactivity was reduced, and the test showed good performance. The limit of detection was reduced to 10 ng/ml in vitro, and the test showed good detection ability in clinical snake envenoming case samples. The ICT-Viper performed well and could be combined with a cobra venom detection kit (ICT-Cobra) to create a multiple detection strip (ICT-VC), which broadens its applications while maintaining its detection ability for snake envenomation identification. Nonetheless, the use of the ICT-Viper in the South-East Asia region is pending additional laboratory and field investigations and regional collaboration. We believe that the development of this practical diagnostic tool marks the beginning of positive efforts to face the global snakebite issue.
format Online
Article
Text
id pubmed-7529284
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-75292842020-10-08 Collocation of avian and mammal antibodies to develop a rapid and sensitive diagnostic tool for Russell's Vipers Snakebite Lin, Jing-Hua Lo, Che-Min Chuang, Ssu-Han Chiang, Chao-Hung Wang, Sheng-Der Lin, Tsung-Yi Liao, Jiunn-Wang Hung, Dong-Zong PLoS Negl Trop Dis Research Article Russell’s vipers (RVs) envenoming is an important public health issue in South-East Asia. Disseminated intravascular coagulopathy, systemic bleeding, hemolysis, and acute renal injury are obvious problems that develop in most cases, and neuromuscular junction blocks are an additional problem caused by western RV snakebite. The complex presentations usually are an obstacle to early diagnosis and antivenom administration. Here, we tried to produce highly specific antibodies in goose yolks for use in a paper-based microfluidic diagnostic kit, immunochromatographic test of viper (ICT-Viper), to distinguish RVs from other vipers and even cobra snakebite in Asia. We used indirect ELISA to monitor specific goose IgY production and western blotting to illustrate the interaction of avian or mammal antibody with venom proteins. The ICT-Viper was tested not only in prepared samples but also in stored patient serum to demonstrate its preliminary efficacy. The results revealed that specific anti-Daboia russelii IgY could be raised in goose eggs effectively without inducing adverse effects. When it was collocated with horse anti-Daboia siamensis antibody, which broadly reacted with most of the venom proteins of both types of Russell’s viper, the false cross-reactivity was reduced, and the test showed good performance. The limit of detection was reduced to 10 ng/ml in vitro, and the test showed good detection ability in clinical snake envenoming case samples. The ICT-Viper performed well and could be combined with a cobra venom detection kit (ICT-Cobra) to create a multiple detection strip (ICT-VC), which broadens its applications while maintaining its detection ability for snake envenomation identification. Nonetheless, the use of the ICT-Viper in the South-East Asia region is pending additional laboratory and field investigations and regional collaboration. We believe that the development of this practical diagnostic tool marks the beginning of positive efforts to face the global snakebite issue. Public Library of Science 2020-09-21 /pmc/articles/PMC7529284/ /pubmed/32956365 http://dx.doi.org/10.1371/journal.pntd.0008701 Text en © 2020 Lin et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lin, Jing-Hua
Lo, Che-Min
Chuang, Ssu-Han
Chiang, Chao-Hung
Wang, Sheng-Der
Lin, Tsung-Yi
Liao, Jiunn-Wang
Hung, Dong-Zong
Collocation of avian and mammal antibodies to develop a rapid and sensitive diagnostic tool for Russell's Vipers Snakebite
title Collocation of avian and mammal antibodies to develop a rapid and sensitive diagnostic tool for Russell's Vipers Snakebite
title_full Collocation of avian and mammal antibodies to develop a rapid and sensitive diagnostic tool for Russell's Vipers Snakebite
title_fullStr Collocation of avian and mammal antibodies to develop a rapid and sensitive diagnostic tool for Russell's Vipers Snakebite
title_full_unstemmed Collocation of avian and mammal antibodies to develop a rapid and sensitive diagnostic tool for Russell's Vipers Snakebite
title_short Collocation of avian and mammal antibodies to develop a rapid and sensitive diagnostic tool for Russell's Vipers Snakebite
title_sort collocation of avian and mammal antibodies to develop a rapid and sensitive diagnostic tool for russell's vipers snakebite
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529284/
https://www.ncbi.nlm.nih.gov/pubmed/32956365
http://dx.doi.org/10.1371/journal.pntd.0008701
work_keys_str_mv AT linjinghua collocationofavianandmammalantibodiestodeveloparapidandsensitivediagnostictoolforrussellsviperssnakebite
AT lochemin collocationofavianandmammalantibodiestodeveloparapidandsensitivediagnostictoolforrussellsviperssnakebite
AT chuangssuhan collocationofavianandmammalantibodiestodeveloparapidandsensitivediagnostictoolforrussellsviperssnakebite
AT chiangchaohung collocationofavianandmammalantibodiestodeveloparapidandsensitivediagnostictoolforrussellsviperssnakebite
AT wangshengder collocationofavianandmammalantibodiestodeveloparapidandsensitivediagnostictoolforrussellsviperssnakebite
AT lintsungyi collocationofavianandmammalantibodiestodeveloparapidandsensitivediagnostictoolforrussellsviperssnakebite
AT liaojiunnwang collocationofavianandmammalantibodiestodeveloparapidandsensitivediagnostictoolforrussellsviperssnakebite
AT hungdongzong collocationofavianandmammalantibodiestodeveloparapidandsensitivediagnostictoolforrussellsviperssnakebite