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Serum and tissue expression of neuropilin 1 in precancerous and malignant vocal fold lesions

OBJECTIVES: The study was designed to evaluate the tissue expression of NRP-1 and serum level of sNRP-1 in the same patients with intraepithelial laryngeal lesions or early staged laryngeal cancer to identify the clinical significance of these biomarkers in the diagnosis of laryngeal lesions. MATERI...

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Autores principales: Rzepakowska, Anna, Żurek, Michał, Grzybowski, Jakub, Kotula, Iwona, Pihowicz, Paweł, Górnicka, Barbara, Demkow, Urszula, Niemczyk, Kazimierz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529309/
https://www.ncbi.nlm.nih.gov/pubmed/33002021
http://dx.doi.org/10.1371/journal.pone.0239550
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author Rzepakowska, Anna
Żurek, Michał
Grzybowski, Jakub
Kotula, Iwona
Pihowicz, Paweł
Górnicka, Barbara
Demkow, Urszula
Niemczyk, Kazimierz
author_facet Rzepakowska, Anna
Żurek, Michał
Grzybowski, Jakub
Kotula, Iwona
Pihowicz, Paweł
Górnicka, Barbara
Demkow, Urszula
Niemczyk, Kazimierz
author_sort Rzepakowska, Anna
collection PubMed
description OBJECTIVES: The study was designed to evaluate the tissue expression of NRP-1 and serum level of sNRP-1 in the same patients with intraepithelial laryngeal lesions or early staged laryngeal cancer to identify the clinical significance of these biomarkers in the diagnosis of laryngeal lesions. MATERIAL AND METHODS: A prospective analysis of tissue was performed on specimens and blood samples from 49 patients, who were admitted for surgical resection due to suspicious vocal fold lesions and were diagnosed as non-dysplasia, low-grade dysplasia, high-grade dysplasia and invasive cancers. RESULTS: ELISA was conducted on 48 blood samples. The minimum level of sNRP-1 was 0.15 ng/ml and maximum– 37.71 ng/ml. The Kruskal–Wallis one-way analysis of variance revealed no differences in sNRP-1 levels between different histopathological stages of vocal fold lesions (p = 0.234). IHC was conducted in 49 tissue samples. The evaluated mean scores of NRP-1 tissue expression were compared to histopathological stage of the lesion. The Kruskal–Wallis one-way analysis of variance revealed no differences in NRP-1 tissue expression between different histopathological stages of vocal fold lesions (p = 0.536). The correlation of tissue NRP-1 expression and serum levels of NRP-1 within analyzed group was insignificant. The Spearman’s rank correlation coefficient was 0.076 (p = 0.606). CONCLUSIONS: The NRP-1 tissue expression and serum levels are unlikely to be a prognostic factor for identification of laryngeal dysplasia or early stage laryngeal cancer. Further studies investigating biomolecules involved in laryngeal carcinogenesis are necessary.
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spelling pubmed-75293092020-10-08 Serum and tissue expression of neuropilin 1 in precancerous and malignant vocal fold lesions Rzepakowska, Anna Żurek, Michał Grzybowski, Jakub Kotula, Iwona Pihowicz, Paweł Górnicka, Barbara Demkow, Urszula Niemczyk, Kazimierz PLoS One Research Article OBJECTIVES: The study was designed to evaluate the tissue expression of NRP-1 and serum level of sNRP-1 in the same patients with intraepithelial laryngeal lesions or early staged laryngeal cancer to identify the clinical significance of these biomarkers in the diagnosis of laryngeal lesions. MATERIAL AND METHODS: A prospective analysis of tissue was performed on specimens and blood samples from 49 patients, who were admitted for surgical resection due to suspicious vocal fold lesions and were diagnosed as non-dysplasia, low-grade dysplasia, high-grade dysplasia and invasive cancers. RESULTS: ELISA was conducted on 48 blood samples. The minimum level of sNRP-1 was 0.15 ng/ml and maximum– 37.71 ng/ml. The Kruskal–Wallis one-way analysis of variance revealed no differences in sNRP-1 levels between different histopathological stages of vocal fold lesions (p = 0.234). IHC was conducted in 49 tissue samples. The evaluated mean scores of NRP-1 tissue expression were compared to histopathological stage of the lesion. The Kruskal–Wallis one-way analysis of variance revealed no differences in NRP-1 tissue expression between different histopathological stages of vocal fold lesions (p = 0.536). The correlation of tissue NRP-1 expression and serum levels of NRP-1 within analyzed group was insignificant. The Spearman’s rank correlation coefficient was 0.076 (p = 0.606). CONCLUSIONS: The NRP-1 tissue expression and serum levels are unlikely to be a prognostic factor for identification of laryngeal dysplasia or early stage laryngeal cancer. Further studies investigating biomolecules involved in laryngeal carcinogenesis are necessary. Public Library of Science 2020-10-01 /pmc/articles/PMC7529309/ /pubmed/33002021 http://dx.doi.org/10.1371/journal.pone.0239550 Text en © 2020 Rzepakowska et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rzepakowska, Anna
Żurek, Michał
Grzybowski, Jakub
Kotula, Iwona
Pihowicz, Paweł
Górnicka, Barbara
Demkow, Urszula
Niemczyk, Kazimierz
Serum and tissue expression of neuropilin 1 in precancerous and malignant vocal fold lesions
title Serum and tissue expression of neuropilin 1 in precancerous and malignant vocal fold lesions
title_full Serum and tissue expression of neuropilin 1 in precancerous and malignant vocal fold lesions
title_fullStr Serum and tissue expression of neuropilin 1 in precancerous and malignant vocal fold lesions
title_full_unstemmed Serum and tissue expression of neuropilin 1 in precancerous and malignant vocal fold lesions
title_short Serum and tissue expression of neuropilin 1 in precancerous and malignant vocal fold lesions
title_sort serum and tissue expression of neuropilin 1 in precancerous and malignant vocal fold lesions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529309/
https://www.ncbi.nlm.nih.gov/pubmed/33002021
http://dx.doi.org/10.1371/journal.pone.0239550
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