Protocol for Cloning Epithelial Stem Cell Variants from Human Lung

The plurality of clonogenic cells derived from human lung includes a spectrum of diverse p63+ stem cells responsible for the regeneration of normal epithelial tissue and disease-associated metaplastic lesions. Here, we report protocols for the cloning, expansion, and characterization of these stem c...

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Autores principales: Rao, Wei, Niroula, Suchan, Wang, Shan, Vincent, Matthew, McKeon, Frank, Xian, Wa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529324/
https://www.ncbi.nlm.nih.gov/pubmed/33015646
http://dx.doi.org/10.1016/j.xpro.2020.100063
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author Rao, Wei
Niroula, Suchan
Wang, Shan
Vincent, Matthew
McKeon, Frank
Xian, Wa
author_facet Rao, Wei
Niroula, Suchan
Wang, Shan
Vincent, Matthew
McKeon, Frank
Xian, Wa
author_sort Rao, Wei
collection PubMed
description The plurality of clonogenic cells derived from human lung includes a spectrum of diverse p63+ stem cells responsible for the regeneration of normal epithelial tissue and disease-associated metaplastic lesions. Here, we report protocols for the cloning, expansion, and characterization of these stem cell variants, which in general assist in analyses of stem cell heterogeneity, genome editing, drug screening, and regenerative medicine. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2011), Zuo et al. (2015), and Rao et al. (2020).
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spelling pubmed-75293242020-10-01 Protocol for Cloning Epithelial Stem Cell Variants from Human Lung Rao, Wei Niroula, Suchan Wang, Shan Vincent, Matthew McKeon, Frank Xian, Wa STAR Protoc Protocol The plurality of clonogenic cells derived from human lung includes a spectrum of diverse p63+ stem cells responsible for the regeneration of normal epithelial tissue and disease-associated metaplastic lesions. Here, we report protocols for the cloning, expansion, and characterization of these stem cell variants, which in general assist in analyses of stem cell heterogeneity, genome editing, drug screening, and regenerative medicine. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2011), Zuo et al. (2015), and Rao et al. (2020). Elsevier 2020-07-09 /pmc/articles/PMC7529324/ /pubmed/33015646 http://dx.doi.org/10.1016/j.xpro.2020.100063 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Rao, Wei
Niroula, Suchan
Wang, Shan
Vincent, Matthew
McKeon, Frank
Xian, Wa
Protocol for Cloning Epithelial Stem Cell Variants from Human Lung
title Protocol for Cloning Epithelial Stem Cell Variants from Human Lung
title_full Protocol for Cloning Epithelial Stem Cell Variants from Human Lung
title_fullStr Protocol for Cloning Epithelial Stem Cell Variants from Human Lung
title_full_unstemmed Protocol for Cloning Epithelial Stem Cell Variants from Human Lung
title_short Protocol for Cloning Epithelial Stem Cell Variants from Human Lung
title_sort protocol for cloning epithelial stem cell variants from human lung
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529324/
https://www.ncbi.nlm.nih.gov/pubmed/33015646
http://dx.doi.org/10.1016/j.xpro.2020.100063
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