Cargando…
Molecular characterization of canine coronaviruses: an enteric and pantropic approach
Canine coronavirus (CCoV) generally causes an infection with high morbidity and low mortality in dogs. In recent years, studies on coronaviruses have gained a momentum due to coronavirus outbreaks. Mutations in coronaviruses can result in deadly diseases in new hosts (such as SARS-CoV-2) or cause ch...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Vienna
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529357/ https://www.ncbi.nlm.nih.gov/pubmed/33005986 http://dx.doi.org/10.1007/s00705-020-04826-w |
_version_ | 1783589419711725568 |
---|---|
author | Timurkan, Mehmet Ozkan Aydin, Hakan Dincer, Ender Coskun, Nuvit |
author_facet | Timurkan, Mehmet Ozkan Aydin, Hakan Dincer, Ender Coskun, Nuvit |
author_sort | Timurkan, Mehmet Ozkan |
collection | PubMed |
description | Canine coronavirus (CCoV) generally causes an infection with high morbidity and low mortality in dogs. In recent years, studies on coronaviruses have gained a momentum due to coronavirus outbreaks. Mutations in coronaviruses can result in deadly diseases in new hosts (such as SARS-CoV-2) or cause changes in organ-tissue affinity, as occurred with feline infectious peritonitis virus, exacerbating their pathogenesis. In recent studies on different types of CCoV, the pantropic strains characterized by hypervirulent and multi-systemic infections are believed to be emerging, in contrast to classical enteric coronavirus infections. In this study, we investigated emerging hypervirulent and multi-systemic CCoV strains using molecular and bioinformatic analysis, and examined differences between enteric and pantropic CCoV strains at the phylogenetic level. RT-PCR was performed with specific primers to identify the coronavirus M (membrane) and S (spike) genes, and samples were then subjected to DNA sequencing. In phylogenetic analysis, four out of 26 samples were classified as CCoV-1. The remaining 22 samples were all classified as CCoV-2a. In the CCoV-2a group, six samples were in branches close to enteric strains, and 16 samples were in the branches close to pantropic strains. Enteric and pantropic strains were compared by molecular genotyping of CCoV in dogs. Phylogenetic analysis of hypervirulent pantropic strains was carried out at the amino acid and nucleotide sequence levels. CCoV was found to be divergent from the original strain. This implies that some CCoV strains have become pantropic strains that cause multisystemic infections, and they should not be ruled out as the cause of severe diarrhea and multisystemic infections. |
format | Online Article Text |
id | pubmed-7529357 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Vienna |
record_format | MEDLINE/PubMed |
spelling | pubmed-75293572020-10-02 Molecular characterization of canine coronaviruses: an enteric and pantropic approach Timurkan, Mehmet Ozkan Aydin, Hakan Dincer, Ender Coskun, Nuvit Arch Virol Original Article Canine coronavirus (CCoV) generally causes an infection with high morbidity and low mortality in dogs. In recent years, studies on coronaviruses have gained a momentum due to coronavirus outbreaks. Mutations in coronaviruses can result in deadly diseases in new hosts (such as SARS-CoV-2) or cause changes in organ-tissue affinity, as occurred with feline infectious peritonitis virus, exacerbating their pathogenesis. In recent studies on different types of CCoV, the pantropic strains characterized by hypervirulent and multi-systemic infections are believed to be emerging, in contrast to classical enteric coronavirus infections. In this study, we investigated emerging hypervirulent and multi-systemic CCoV strains using molecular and bioinformatic analysis, and examined differences between enteric and pantropic CCoV strains at the phylogenetic level. RT-PCR was performed with specific primers to identify the coronavirus M (membrane) and S (spike) genes, and samples were then subjected to DNA sequencing. In phylogenetic analysis, four out of 26 samples were classified as CCoV-1. The remaining 22 samples were all classified as CCoV-2a. In the CCoV-2a group, six samples were in branches close to enteric strains, and 16 samples were in the branches close to pantropic strains. Enteric and pantropic strains were compared by molecular genotyping of CCoV in dogs. Phylogenetic analysis of hypervirulent pantropic strains was carried out at the amino acid and nucleotide sequence levels. CCoV was found to be divergent from the original strain. This implies that some CCoV strains have become pantropic strains that cause multisystemic infections, and they should not be ruled out as the cause of severe diarrhea and multisystemic infections. Springer Vienna 2020-10-01 2021 /pmc/articles/PMC7529357/ /pubmed/33005986 http://dx.doi.org/10.1007/s00705-020-04826-w Text en © Springer-Verlag GmbH Austria, part of Springer Nature 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Timurkan, Mehmet Ozkan Aydin, Hakan Dincer, Ender Coskun, Nuvit Molecular characterization of canine coronaviruses: an enteric and pantropic approach |
title | Molecular characterization of canine coronaviruses: an enteric and pantropic approach |
title_full | Molecular characterization of canine coronaviruses: an enteric and pantropic approach |
title_fullStr | Molecular characterization of canine coronaviruses: an enteric and pantropic approach |
title_full_unstemmed | Molecular characterization of canine coronaviruses: an enteric and pantropic approach |
title_short | Molecular characterization of canine coronaviruses: an enteric and pantropic approach |
title_sort | molecular characterization of canine coronaviruses: an enteric and pantropic approach |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529357/ https://www.ncbi.nlm.nih.gov/pubmed/33005986 http://dx.doi.org/10.1007/s00705-020-04826-w |
work_keys_str_mv | AT timurkanmehmetozkan molecularcharacterizationofcaninecoronavirusesanentericandpantropicapproach AT aydinhakan molecularcharacterizationofcaninecoronavirusesanentericandpantropicapproach AT dincerender molecularcharacterizationofcaninecoronavirusesanentericandpantropicapproach AT coskunnuvit molecularcharacterizationofcaninecoronavirusesanentericandpantropicapproach |