Cargando…
Radionecrosis and cellular changes in small volume stereotactic brain radiosurgery in a porcine model
Stereotactic radiosurgery (SRS) has proven an effective tool for the treatment of brain tumors, arteriovenous malformation, and functional conditions. However, radiation-induced therapeutic effect in viable cells in functional SRS is also suggested. Evaluation of the proposed modulatory effect of ir...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529917/ https://www.ncbi.nlm.nih.gov/pubmed/33004849 http://dx.doi.org/10.1038/s41598-020-72876-w |
_version_ | 1783589503986827264 |
---|---|
author | Zaer, Hamed Glud, Andreas Nørgaard Schneider, Bret M. Lukacova, Slávka Vang Hansen, Kim Adler, John R. Høyer, Morten Jensen, Morten Bjørn Hansen, Rune Hoffmann, Lone Worm, Esben Schjødt Sørensen, Jens Chr. Hedemann Orlowski, Dariusz |
author_facet | Zaer, Hamed Glud, Andreas Nørgaard Schneider, Bret M. Lukacova, Slávka Vang Hansen, Kim Adler, John R. Høyer, Morten Jensen, Morten Bjørn Hansen, Rune Hoffmann, Lone Worm, Esben Schjødt Sørensen, Jens Chr. Hedemann Orlowski, Dariusz |
author_sort | Zaer, Hamed |
collection | PubMed |
description | Stereotactic radiosurgery (SRS) has proven an effective tool for the treatment of brain tumors, arteriovenous malformation, and functional conditions. However, radiation-induced therapeutic effect in viable cells in functional SRS is also suggested. Evaluation of the proposed modulatory effect of irradiation on neuronal activity without causing cellular death requires the knowledge of radiation dose tolerance at very small tissue volume. Therefore, we aimed to establish a porcine model to study the effects of ultra-high radiosurgical doses in small volumes of the brain. Five minipigs received focal stereotactic radiosurgery with single large doses of 40–100 Gy to 5–7.5 mm fields in the left primary motor cortex and the right subcortical white matter, and one animal remained as unirradiated control. The animals were followed-up with serial MRI, PET scans, and histology 6 months post-radiation. We observed a dose-dependent relation of the histological and MRI changes at 6 months post-radiation. The necrotic lesions were seen in the grey matter at 100 Gy and in white matter at 60 Gy. Furthermore, small volume radiosurgery at different dose levels induced vascular, as well as neuronal cell changes and glial cell remodeling. |
format | Online Article Text |
id | pubmed-7529917 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-75299172020-10-02 Radionecrosis and cellular changes in small volume stereotactic brain radiosurgery in a porcine model Zaer, Hamed Glud, Andreas Nørgaard Schneider, Bret M. Lukacova, Slávka Vang Hansen, Kim Adler, John R. Høyer, Morten Jensen, Morten Bjørn Hansen, Rune Hoffmann, Lone Worm, Esben Schjødt Sørensen, Jens Chr. Hedemann Orlowski, Dariusz Sci Rep Article Stereotactic radiosurgery (SRS) has proven an effective tool for the treatment of brain tumors, arteriovenous malformation, and functional conditions. However, radiation-induced therapeutic effect in viable cells in functional SRS is also suggested. Evaluation of the proposed modulatory effect of irradiation on neuronal activity without causing cellular death requires the knowledge of radiation dose tolerance at very small tissue volume. Therefore, we aimed to establish a porcine model to study the effects of ultra-high radiosurgical doses in small volumes of the brain. Five minipigs received focal stereotactic radiosurgery with single large doses of 40–100 Gy to 5–7.5 mm fields in the left primary motor cortex and the right subcortical white matter, and one animal remained as unirradiated control. The animals were followed-up with serial MRI, PET scans, and histology 6 months post-radiation. We observed a dose-dependent relation of the histological and MRI changes at 6 months post-radiation. The necrotic lesions were seen in the grey matter at 100 Gy and in white matter at 60 Gy. Furthermore, small volume radiosurgery at different dose levels induced vascular, as well as neuronal cell changes and glial cell remodeling. Nature Publishing Group UK 2020-10-01 /pmc/articles/PMC7529917/ /pubmed/33004849 http://dx.doi.org/10.1038/s41598-020-72876-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Zaer, Hamed Glud, Andreas Nørgaard Schneider, Bret M. Lukacova, Slávka Vang Hansen, Kim Adler, John R. Høyer, Morten Jensen, Morten Bjørn Hansen, Rune Hoffmann, Lone Worm, Esben Schjødt Sørensen, Jens Chr. Hedemann Orlowski, Dariusz Radionecrosis and cellular changes in small volume stereotactic brain radiosurgery in a porcine model |
title | Radionecrosis and cellular changes in small volume stereotactic brain radiosurgery in a porcine model |
title_full | Radionecrosis and cellular changes in small volume stereotactic brain radiosurgery in a porcine model |
title_fullStr | Radionecrosis and cellular changes in small volume stereotactic brain radiosurgery in a porcine model |
title_full_unstemmed | Radionecrosis and cellular changes in small volume stereotactic brain radiosurgery in a porcine model |
title_short | Radionecrosis and cellular changes in small volume stereotactic brain radiosurgery in a porcine model |
title_sort | radionecrosis and cellular changes in small volume stereotactic brain radiosurgery in a porcine model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529917/ https://www.ncbi.nlm.nih.gov/pubmed/33004849 http://dx.doi.org/10.1038/s41598-020-72876-w |
work_keys_str_mv | AT zaerhamed radionecrosisandcellularchangesinsmallvolumestereotacticbrainradiosurgeryinaporcinemodel AT gludandreasnørgaard radionecrosisandcellularchangesinsmallvolumestereotacticbrainradiosurgeryinaporcinemodel AT schneiderbretm radionecrosisandcellularchangesinsmallvolumestereotacticbrainradiosurgeryinaporcinemodel AT lukacovaslavka radionecrosisandcellularchangesinsmallvolumestereotacticbrainradiosurgeryinaporcinemodel AT vanghansenkim radionecrosisandcellularchangesinsmallvolumestereotacticbrainradiosurgeryinaporcinemodel AT adlerjohnr radionecrosisandcellularchangesinsmallvolumestereotacticbrainradiosurgeryinaporcinemodel AT høyermorten radionecrosisandcellularchangesinsmallvolumestereotacticbrainradiosurgeryinaporcinemodel AT jensenmortenbjørn radionecrosisandcellularchangesinsmallvolumestereotacticbrainradiosurgeryinaporcinemodel AT hansenrune radionecrosisandcellularchangesinsmallvolumestereotacticbrainradiosurgeryinaporcinemodel AT hoffmannlone radionecrosisandcellularchangesinsmallvolumestereotacticbrainradiosurgeryinaporcinemodel AT wormesbenschjødt radionecrosisandcellularchangesinsmallvolumestereotacticbrainradiosurgeryinaporcinemodel AT sørensenjenschrhedemann radionecrosisandcellularchangesinsmallvolumestereotacticbrainradiosurgeryinaporcinemodel AT orlowskidariusz radionecrosisandcellularchangesinsmallvolumestereotacticbrainradiosurgeryinaporcinemodel |