Cargando…

Direct Clinical Evidence Recommending the Use of Proteinase K or Dithiothreitol to Pretreat Sputum for Detection of SARS-CoV-2

One of the primary tools for diagnosing COVID-19 is the nucleic acid-based real-time reverse transcriptase-polymerase chain reaction (RT-PCR) test performed on respiratory specimens. The detection rate of SARS-CoV-2 in lower respiratory specimens (such as sputum) is higher than that for upper respir...

Descripción completa

Detalles Bibliográficos
Autores principales:	Peng, Jing, Lu, Yanjun, Song, Juan, Vallance, Bruce A., Jacobson, Kevan, Yu, Hong Bing, Sun, Ziyong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Medicine
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7530184/ https://www.ncbi.nlm.nih.gov/pubmed/33043036 http://dx.doi.org/10.3389/fmed.2020.549860

Ejemplares similares

The Effects of Dithiothreitol on DNA
por: Fjelstrup, Søren, et al.
Publicado: (2017)

High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitol
por: Papagianni, Maria, et al.
Publicado: (2007)

INHIBITION OF NUCLEAR RECONSTRUCTION BY DITHIOTHREITOL
por: Wolfe, Jason, et al.
Publicado: (1974)

Toxicity of Dithiothreitol (DTT) to Drosophila melanogaster
por: Wang, Yiwen, et al.
Publicado: (2020)

Immobilization of Proteinase K for urine pretreatment to improve diagnostic accuracy of active tuberculosis
por: Panraksa, Yosita, et al.
Publicado: (2021)

Sperm Pretreatment with Dithiothreitol Increases Male Pronucleus Formation Rates After Intracytoplasmic Sperm Injection (ICSI) in Swamp Buffalo Oocytes
por: CHANKITISAKUL, Vibuntita, et al.
Publicado: (2012)

Dithiothreitol and the translocation of preprolactin across mammalian endoplasmic reticulum
Publicado: (1987)

Effects of Dithiothreitol on Fertilization and Early Development in Sea Urchin
por: Limatola, Nunzia, et al.
Publicado: (2021)

Electrocatalytic Depolymerization of Self-Immolative Poly(Dithiothreitol) Derivatives
por: Hansen-Felby, Magnus, et al.
Publicado: (2022)

Dithiothreitol Affects the Fertilization Response in Immature and Maturing Starfish Oocytes
por: Limatola, Nunzia, et al.
Publicado: (2023)

Elastic Fibres in Sputum
Publicado: (1909)

Paradoxical Acceleration of Dithiothreitol-Induced Aggregation of Insulin in the Presence of a Chaperone
por: Bumagina, Zoya, et al.
Publicado: (2010)

A Chemosensor Based on Gold Nanoparticles and Dithiothreitol (DTT) for Acrylamide Electroanalysis
por: Alam, Shahenvaz, et al.
Publicado: (2021)

Dithiothreitol causes toxicity in C. elegans by modulating the methionine–homocysteine cycle
por: G, Gokul, et al.
Publicado: (2022)

Eosinophile Cells in the Sputum in Asthma
Publicado: (1910)

Better resolving of anti‐CD38 antibody interference with blood compatibility testing by using manual polybrene method compared with dithiothreitol‐pretreatment indirect antiglobulin test
por: Feng, Chiu‐Che, et al.
Publicado: (2023)

Performance of Xpert MTB/RIF and sputum microscopy compared to sputum culture for diagnosis of tuberculosis in seven hospitals in Indonesia
por: Karuniawati, Anis, et al.
Publicado: (2023)

Proteinase K-pretreated ConA-based ELISA assay: a novel urine LAM detection strategy for TB diagnosis
por: Huang, Huan, et al.
Publicado: (2023)

A New Method for the Sputum Cytology Test Without Direct Contact to Specimens During COVID-19 Pandemic
por: Cui, Junqi, et al.
Publicado: (2022)

Comparison of two reducing agents dithiothreitol and tris(3-hydroxypropyl)phosphine for in vitro kinetic assay of vitamin K epoxide reductase
por: Takeda, Kazuki, et al.
Publicado: (2020)

Family with sequence similarity 134 member B-mediated reticulophagy ameliorates hepatocyte apoptosis induced by dithiothreitol
por: Guo, Yi-Xin, et al.
Publicado: (2022)

Acid-Fast Bacilli in Non-Tuberculous Sputum
Publicado: (1909)

Proteinase K resistant cores of prions and amyloids
por: Kushnirov, Vitaly V., et al.
Publicado: (2019)

Unfolding and Aggregation of Lysozyme under the Combined Action of Dithiothreitol and Guanidine Hydrochloride: Optical Studies
por: Sarimov, Ruslan M., et al.
Publicado: (2021)

Investigation of the Efficacy of Dithiothreitol and Glutathione on In Vitro Fertilization of Cryopreserved Large White Boar Semen
por: Ledwaba, Mahlatsana Ramaesela, et al.
Publicado: (2022)

Sputasol (Dithiothreitol 0.54%) Improves the Detection of Human Papillomaviruses Using the Cobas 4800 System
por: Wang, Qing-Yong, et al.
Publicado: (2017)

Structural analysis of Cytochrome P450 BM3 mutant M11 in complex with dithiothreitol
por: Frydenvang, Karla, et al.
Publicado: (2019)

Distinct Roles of Nrf1 and Nrf2 in Monitoring the Reductive Stress Response to Dithiothreitol (DTT)
por: Wufuer, Reziyamu, et al.
Publicado: (2022)

Harnessing Big Data to Optimize an Algorithm for Rapid Diagnosis of Pulmonary Tuberculosis in a Real-World Setting
por: Peng, Jing, et al.
Publicado: (2021)

Bloodstained sputum of unknown etiology
por: Fernandes, Filipa, et al.
Publicado: (2017)

SARS-CoV-2 Molecular Diagnostics in China
por: Lu, Yanjun, et al.
Publicado: (2022)

Compare the Effect of Inhaled Corticosteroids and Systemic Corticosteroids on Sputum Microbiome of AECOPD
por: Ma, Nan, et al.
Publicado: (2021)

Vitality of Proteinase K in rRTPCR Detection of SARS-CoV2 Bypassing RNA Extraction
por: Shukla, Alka, et al.
Publicado: (2021)

Proteinases in the joint: clinical relevance of proteinases in joint destruction
por: Rengel, Yvonne, et al.
Publicado: (2007)

Engineering proteinase K using machine learning and synthetic genes
por: Liao, Jun, et al.
Publicado: (2007)

Effect of the Solvent Temperatures on Dynamics of Serine Protease Proteinase K
por: Sang, Peng, et al.
Publicado: (2016)

Circulating autoreactive proteinase 3(+) B cells and tolerance checkpoints in ANCA-associated vasculitis
por: Berti, Alvise, et al.
Publicado: (2021)

Enhancement of Photoluminescence from Semiconducting Nanotubes in Aqueous Suspensions due to Cysteine and Dithiothreitol Doping: Influence of the Sonication Treatment
por: Kurnosov, Nikita V., et al.
Publicado: (2016)

Dithiothreitol‐Measured Oxidative Potential of Size‐Segregated Particulate Matter in Fukuoka, Japan: Effects of Asian Dust Events
por: Nishita‐Hara, Chiharu, et al.
Publicado: (2019)

Dithiothreitol‐Measured Oxidative Potential of Reference Materials of Mineral Dust: Implications for the Toxicity of Mineral Dust Aerosols in the Atmosphere
por: Nishita‐Hara, Chiharu, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_86g17uukc4jnrs22ubf1pe7kah