Development of Digital Image Processing as an Innovative Method for Activated Sludge Biomass Quantification

Activated sludge process is the most common method for biological treatment of industrial and municipal wastewater. One of the most important parameters in performance of activated sludge systems is quantitative monitoring of biomass to keep the cell concentration in an optimum range. In this study, a novel method for activated sludge quantification based on image processing and RGB analysis is proposed. According to the results, the intensity of blue color in the macroscopic image of activated sludge culture can be a very accurate index for cell concentration measurement and R(2) coefficient, Root Mean Square Error (RMSE), Mean Absolute Error (MAE), and Mean Absolute Percentage Error (MAPE) which are 0.990, 2.000, 0.323, and 13.848, respectively, prove this claim. Besides, in order to avoid the difficulties of working in the three-parameter space of RGB, converting to grayscale space has been applied which can estimate cell concentration with R(2) = 0.99. Ultimately, an exponential correlation between RGB values and cell concentrations in lower amounts of biomass has been proposed based on Beer-Lambert law which can estimate activated sludge biomass concentration with R(2) = 0.97 based on B index.