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Microarray‐based transcriptional profiling of a mouse model of autoimmune hepatitis
Long noncoding RNAs (lncRNAs) are RNA molecules longer than 200 nucleotides that do not typically code for a protein. lncRNAs have regulatory roles in many physiological processes, and their dysregulation can contribute to cancer, cardiovascular and neurodegenerative diseases, as well as the onset o...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7530384/ https://www.ncbi.nlm.nih.gov/pubmed/32808463 http://dx.doi.org/10.1002/2211-5463.12953 |
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author | Liu, Yang Chen, Hao Hao, Jian‐heng Li, Zhen‐cheng Hou, Tiezheng Hao, Hui‐qin |
author_facet | Liu, Yang Chen, Hao Hao, Jian‐heng Li, Zhen‐cheng Hou, Tiezheng Hao, Hui‐qin |
author_sort | Liu, Yang |
collection | PubMed |
description | Long noncoding RNAs (lncRNAs) are RNA molecules longer than 200 nucleotides that do not typically code for a protein. lncRNAs have regulatory roles in many physiological processes, and their dysregulation can contribute to cancer, cardiovascular and neurodegenerative diseases, as well as the onset of autoimmune diseases, including systemic lupus erythematosus and rheumatoid arthritis. However, lncRNA expression changes in autoimmune hepatitis (AIH), a form of inflammation induced by immunological tolerance disorders, are poorly understood. Here, for the first time to our knowledge, we used microarrays to profile 1161 differentially expressed lncRNAs (DELs; 608 up‐ and 553 down‐regulated) and 11 512 differentially expressed mRNAs (DEMs; 5189 up‐ and 6323 down‐ regulated) in a concanavalin A‐induced AIH mouse model. We used quantitative real‐time PCR to confirm the expression of eight DELs and DEMs, and analyzed the coexpression relationship between them. Potential biological functions of screened DELs and DEMs were predicted with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis. DEL‐DEM interaction networks were also constructed. Our study revealed the roles of DELs and DEMs in the pathogenesis of AIH. We also provided potential candidate biomarkers that may have potential for future development into possible diagnostics or as a treatment for this disorder. |
format | Online Article Text |
id | pubmed-7530384 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75303842020-10-05 Microarray‐based transcriptional profiling of a mouse model of autoimmune hepatitis Liu, Yang Chen, Hao Hao, Jian‐heng Li, Zhen‐cheng Hou, Tiezheng Hao, Hui‐qin FEBS Open Bio Research Articles Long noncoding RNAs (lncRNAs) are RNA molecules longer than 200 nucleotides that do not typically code for a protein. lncRNAs have regulatory roles in many physiological processes, and their dysregulation can contribute to cancer, cardiovascular and neurodegenerative diseases, as well as the onset of autoimmune diseases, including systemic lupus erythematosus and rheumatoid arthritis. However, lncRNA expression changes in autoimmune hepatitis (AIH), a form of inflammation induced by immunological tolerance disorders, are poorly understood. Here, for the first time to our knowledge, we used microarrays to profile 1161 differentially expressed lncRNAs (DELs; 608 up‐ and 553 down‐regulated) and 11 512 differentially expressed mRNAs (DEMs; 5189 up‐ and 6323 down‐ regulated) in a concanavalin A‐induced AIH mouse model. We used quantitative real‐time PCR to confirm the expression of eight DELs and DEMs, and analyzed the coexpression relationship between them. Potential biological functions of screened DELs and DEMs were predicted with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis. DEL‐DEM interaction networks were also constructed. Our study revealed the roles of DELs and DEMs in the pathogenesis of AIH. We also provided potential candidate biomarkers that may have potential for future development into possible diagnostics or as a treatment for this disorder. John Wiley and Sons Inc. 2020-09-19 /pmc/articles/PMC7530384/ /pubmed/32808463 http://dx.doi.org/10.1002/2211-5463.12953 Text en © 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Liu, Yang Chen, Hao Hao, Jian‐heng Li, Zhen‐cheng Hou, Tiezheng Hao, Hui‐qin Microarray‐based transcriptional profiling of a mouse model of autoimmune hepatitis |
title | Microarray‐based transcriptional profiling of a mouse model of autoimmune hepatitis |
title_full | Microarray‐based transcriptional profiling of a mouse model of autoimmune hepatitis |
title_fullStr | Microarray‐based transcriptional profiling of a mouse model of autoimmune hepatitis |
title_full_unstemmed | Microarray‐based transcriptional profiling of a mouse model of autoimmune hepatitis |
title_short | Microarray‐based transcriptional profiling of a mouse model of autoimmune hepatitis |
title_sort | microarray‐based transcriptional profiling of a mouse model of autoimmune hepatitis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7530384/ https://www.ncbi.nlm.nih.gov/pubmed/32808463 http://dx.doi.org/10.1002/2211-5463.12953 |
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