SENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells

Pyroptosis is a kind of necrotic and inflammatory programmed cell death induced by inflammatory caspases. SENP7 is a SUMO-specific protease, which mainly acts on deconjugation of SUMOs from substrate proteins. We evaluated the effect of SENP7 knockdown on pyroptosis, NF-κB signaling pathway, and NLR...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Xun, Jiao, Fangzhou, Hong, Jia, Yang, Fan, Wang, Luwen, Gong, Zuojiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7530744/
https://www.ncbi.nlm.nih.gov/pubmed/33004957
http://dx.doi.org/10.1038/s41598-020-73400-w
_version_ 1783589630340235264
author Li, Xun
Jiao, Fangzhou
Hong, Jia
Yang, Fan
Wang, Luwen
Gong, Zuojiong
author_facet Li, Xun
Jiao, Fangzhou
Hong, Jia
Yang, Fan
Wang, Luwen
Gong, Zuojiong
author_sort Li, Xun
collection PubMed
description Pyroptosis is a kind of necrotic and inflammatory programmed cell death induced by inflammatory caspases. SENP7 is a SUMO-specific protease, which mainly acts on deconjugation of SUMOs from substrate proteins. We evaluated the effect of SENP7 knockdown on pyroptosis, NF-κB signaling pathway, and NLRP3 inflammasome in Raw 264.7 cells. The results showed that the GSDMD protein mainly expressed in the cytoplasm nearby nuclei of Raw 264.7 cells. It migrated to cytomembrane with the numbers of Raw 264.7 cell decreased when LPS + ATP were administrated. Which was inhibited by SENP7 knockdown. In addition, not only the pyroptosis of Raw 264.7 cells was inhibited, the activation of NF-κB signaling pathway and NLRP3 inflammasome were also attenuated by SENP7 knockdown. The mechanism may be associated with the over SUMOylation of proteins induced by SENP7 knockdown.
format Online
Article
Text
id pubmed-7530744
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-75307442020-10-02 SENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells Li, Xun Jiao, Fangzhou Hong, Jia Yang, Fan Wang, Luwen Gong, Zuojiong Sci Rep Article Pyroptosis is a kind of necrotic and inflammatory programmed cell death induced by inflammatory caspases. SENP7 is a SUMO-specific protease, which mainly acts on deconjugation of SUMOs from substrate proteins. We evaluated the effect of SENP7 knockdown on pyroptosis, NF-κB signaling pathway, and NLRP3 inflammasome in Raw 264.7 cells. The results showed that the GSDMD protein mainly expressed in the cytoplasm nearby nuclei of Raw 264.7 cells. It migrated to cytomembrane with the numbers of Raw 264.7 cell decreased when LPS + ATP were administrated. Which was inhibited by SENP7 knockdown. In addition, not only the pyroptosis of Raw 264.7 cells was inhibited, the activation of NF-κB signaling pathway and NLRP3 inflammasome were also attenuated by SENP7 knockdown. The mechanism may be associated with the over SUMOylation of proteins induced by SENP7 knockdown. Nature Publishing Group UK 2020-10-01 /pmc/articles/PMC7530744/ /pubmed/33004957 http://dx.doi.org/10.1038/s41598-020-73400-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Xun
Jiao, Fangzhou
Hong, Jia
Yang, Fan
Wang, Luwen
Gong, Zuojiong
SENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells
title SENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells
title_full SENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells
title_fullStr SENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells
title_full_unstemmed SENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells
title_short SENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells
title_sort senp7 knockdown inhibited pyroptosis and nf-κb/nlrp3 inflammasome pathway activation in raw 264.7 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7530744/
https://www.ncbi.nlm.nih.gov/pubmed/33004957
http://dx.doi.org/10.1038/s41598-020-73400-w
work_keys_str_mv AT lixun senp7knockdowninhibitedpyroptosisandnfkbnlrp3inflammasomepathwayactivationinraw2647cells
AT jiaofangzhou senp7knockdowninhibitedpyroptosisandnfkbnlrp3inflammasomepathwayactivationinraw2647cells
AT hongjia senp7knockdowninhibitedpyroptosisandnfkbnlrp3inflammasomepathwayactivationinraw2647cells
AT yangfan senp7knockdowninhibitedpyroptosisandnfkbnlrp3inflammasomepathwayactivationinraw2647cells
AT wangluwen senp7knockdowninhibitedpyroptosisandnfkbnlrp3inflammasomepathwayactivationinraw2647cells
AT gongzuojiong senp7knockdowninhibitedpyroptosisandnfkbnlrp3inflammasomepathwayactivationinraw2647cells

Ejemplares similares