SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene

We found that a single nucleotide polymorphism (SNP) in the nucleoprotein gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from a patient interfered with detection in a widely used commercial assay. Some 0.2% of the isolates in the EpiCoV database contain this SNP. Although SARS-...

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Autores principales: Ziegler, Katharina, Steininger, Philipp, Ziegler, Renate, Steinmann, Jörg, Korn, Klaus, Ensser, Armin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2020
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7531073/
https://www.ncbi.nlm.nih.gov/pubmed/33006300
http://dx.doi.org/10.2807/1560-7917.ES.2020.25.39.2001650
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author Ziegler, Katharina
Steininger, Philipp
Ziegler, Renate
Steinmann, Jörg
Korn, Klaus
Ensser, Armin
author_facet Ziegler, Katharina
Steininger, Philipp
Ziegler, Renate
Steinmann, Jörg
Korn, Klaus
Ensser, Armin
author_sort Ziegler, Katharina
collection PubMed
description We found that a single nucleotide polymorphism (SNP) in the nucleoprotein gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from a patient interfered with detection in a widely used commercial assay. Some 0.2% of the isolates in the EpiCoV database contain this SNP. Although SARS-CoV-2 was still detected by the other probe in the assay, this underlines the necessity of targeting two independent essential regions of a pathogen for reliable detection.
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spelling pubmed-75310732020-10-13 SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene Ziegler, Katharina Steininger, Philipp Ziegler, Renate Steinmann, Jörg Korn, Klaus Ensser, Armin Euro Surveill Rapid Communication We found that a single nucleotide polymorphism (SNP) in the nucleoprotein gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from a patient interfered with detection in a widely used commercial assay. Some 0.2% of the isolates in the EpiCoV database contain this SNP. Although SARS-CoV-2 was still detected by the other probe in the assay, this underlines the necessity of targeting two independent essential regions of a pathogen for reliable detection. European Centre for Disease Prevention and Control (ECDC) 2020-10-01 /pmc/articles/PMC7531073/ /pubmed/33006300 http://dx.doi.org/10.2807/1560-7917.ES.2020.25.39.2001650 Text en This article is copyright of the authors or their affiliated institutions, 2020. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.
spellingShingle Rapid Communication
Ziegler, Katharina
Steininger, Philipp
Ziegler, Renate
Steinmann, Jörg
Korn, Klaus
Ensser, Armin
SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene
title SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene
title_full SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene
title_fullStr SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene
title_full_unstemmed SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene
title_short SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene
title_sort sars-cov-2 samples may escape detection because of a single point mutation in the n gene
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7531073/
https://www.ncbi.nlm.nih.gov/pubmed/33006300
http://dx.doi.org/10.2807/1560-7917.ES.2020.25.39.2001650
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