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Molecular characterization of clinical and environmental Vibrio parahaemolyticus isolates in Huzhou, China

Vibrio parahaemolyticus is responsible for seafood-borne gastroenteritis worldwide. Isolates of V. parahaemolyticus from clinical samples (n = 54) and environmental samples (n = 38) in Huzhou were analyzed by serological typing, virulence gene detection, antibiotic resistance testing, and pulsed-fie...

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Autores principales: Yan, Wei, Ji, Lei, Xu, Deshun, Chen, Liping, Wu, Xiaofang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7531842/
https://www.ncbi.nlm.nih.gov/pubmed/33007026
http://dx.doi.org/10.1371/journal.pone.0240143
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author Yan, Wei
Ji, Lei
Xu, Deshun
Chen, Liping
Wu, Xiaofang
author_facet Yan, Wei
Ji, Lei
Xu, Deshun
Chen, Liping
Wu, Xiaofang
author_sort Yan, Wei
collection PubMed
description Vibrio parahaemolyticus is responsible for seafood-borne gastroenteritis worldwide. Isolates of V. parahaemolyticus from clinical samples (n = 54) and environmental samples (n = 38) in Huzhou were analyzed by serological typing, virulence gene detection, antibiotic resistance testing, and pulsed-field gel electrophoresis (PFGE) for molecular typing. O3:K6 was the main serotype and tlh+tdh+trh- was the most frequently detected virulence genotype in clinical strains. O2:Kut was the main serotype and tlh+tdh-trh- was the most frequently detected virulence genotype in environmental strains. Antibiotic resistance testing indicated that the isolates were highly resistant to ampicillin (90.76%), followed by gentamicin and tetracycline. Following the restriction enzyme NotI digestion, the 91 strains yielded 81 PFGE patterns, and 16 clones had similarity values of > 85.00%, indicating a high level of diversity. Finally, there may be cross-contamination between freshwater and seawater products, so it is necessary to strengthen supervision of food processing.
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spelling pubmed-75318422020-10-08 Molecular characterization of clinical and environmental Vibrio parahaemolyticus isolates in Huzhou, China Yan, Wei Ji, Lei Xu, Deshun Chen, Liping Wu, Xiaofang PLoS One Research Article Vibrio parahaemolyticus is responsible for seafood-borne gastroenteritis worldwide. Isolates of V. parahaemolyticus from clinical samples (n = 54) and environmental samples (n = 38) in Huzhou were analyzed by serological typing, virulence gene detection, antibiotic resistance testing, and pulsed-field gel electrophoresis (PFGE) for molecular typing. O3:K6 was the main serotype and tlh+tdh+trh- was the most frequently detected virulence genotype in clinical strains. O2:Kut was the main serotype and tlh+tdh-trh- was the most frequently detected virulence genotype in environmental strains. Antibiotic resistance testing indicated that the isolates were highly resistant to ampicillin (90.76%), followed by gentamicin and tetracycline. Following the restriction enzyme NotI digestion, the 91 strains yielded 81 PFGE patterns, and 16 clones had similarity values of > 85.00%, indicating a high level of diversity. Finally, there may be cross-contamination between freshwater and seawater products, so it is necessary to strengthen supervision of food processing. Public Library of Science 2020-10-02 /pmc/articles/PMC7531842/ /pubmed/33007026 http://dx.doi.org/10.1371/journal.pone.0240143 Text en © 2020 Yan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yan, Wei
Ji, Lei
Xu, Deshun
Chen, Liping
Wu, Xiaofang
Molecular characterization of clinical and environmental Vibrio parahaemolyticus isolates in Huzhou, China
title Molecular characterization of clinical and environmental Vibrio parahaemolyticus isolates in Huzhou, China
title_full Molecular characterization of clinical and environmental Vibrio parahaemolyticus isolates in Huzhou, China
title_fullStr Molecular characterization of clinical and environmental Vibrio parahaemolyticus isolates in Huzhou, China
title_full_unstemmed Molecular characterization of clinical and environmental Vibrio parahaemolyticus isolates in Huzhou, China
title_short Molecular characterization of clinical and environmental Vibrio parahaemolyticus isolates in Huzhou, China
title_sort molecular characterization of clinical and environmental vibrio parahaemolyticus isolates in huzhou, china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7531842/
https://www.ncbi.nlm.nih.gov/pubmed/33007026
http://dx.doi.org/10.1371/journal.pone.0240143
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