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Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex
Mycobacterium abscessus complex (MABC) is a taxonomic group of rapidly growing, nontuberculous mycobacteria that are found as etiologic agents of various types of infections. They are considered as emerging human pathogens. MABC consists of 3 subspecies—M. abscessus subsp. bolletti, M. abscessus sub...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532137/ https://www.ncbi.nlm.nih.gov/pubmed/33009494 http://dx.doi.org/10.1038/s41598-020-73607-x |
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author | Minias, Alina Żukowska, Lidia Lach, Jakub Jagielski, Tomasz Strapagiel, Dominik Kim, Su-Young Koh, Won-Jung Adam, Heather Bittner, Ruth Truden, Sara Žolnir-Dovč, Manca Dziadek, Jarosław |
author_facet | Minias, Alina Żukowska, Lidia Lach, Jakub Jagielski, Tomasz Strapagiel, Dominik Kim, Su-Young Koh, Won-Jung Adam, Heather Bittner, Ruth Truden, Sara Žolnir-Dovč, Manca Dziadek, Jarosław |
author_sort | Minias, Alina |
collection | PubMed |
description | Mycobacterium abscessus complex (MABC) is a taxonomic group of rapidly growing, nontuberculous mycobacteria that are found as etiologic agents of various types of infections. They are considered as emerging human pathogens. MABC consists of 3 subspecies—M. abscessus subsp. bolletti, M. abscessus subsp. massiliense and M. abscessus subsp. abscessus. Here we present a novel method for subspecies differentiation of M. abscessus named Subspecies-Specific Sequence Detection (SSSD). This method is based on the presence of signature sequences present within the genomes of each subspecies of MABC. We tested this method against a virtual database of 1505 genome sequences of MABC. Further, we detected signature sequences of MABC in 45 microbiological samples through DNA hybridization. SSSD showed high levels of sensitivity and specificity for differentiation of subspecies of MABC, comparable to those obtained by rpoB sequence typing. |
format | Online Article Text |
id | pubmed-7532137 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-75321372020-10-06 Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex Minias, Alina Żukowska, Lidia Lach, Jakub Jagielski, Tomasz Strapagiel, Dominik Kim, Su-Young Koh, Won-Jung Adam, Heather Bittner, Ruth Truden, Sara Žolnir-Dovč, Manca Dziadek, Jarosław Sci Rep Article Mycobacterium abscessus complex (MABC) is a taxonomic group of rapidly growing, nontuberculous mycobacteria that are found as etiologic agents of various types of infections. They are considered as emerging human pathogens. MABC consists of 3 subspecies—M. abscessus subsp. bolletti, M. abscessus subsp. massiliense and M. abscessus subsp. abscessus. Here we present a novel method for subspecies differentiation of M. abscessus named Subspecies-Specific Sequence Detection (SSSD). This method is based on the presence of signature sequences present within the genomes of each subspecies of MABC. We tested this method against a virtual database of 1505 genome sequences of MABC. Further, we detected signature sequences of MABC in 45 microbiological samples through DNA hybridization. SSSD showed high levels of sensitivity and specificity for differentiation of subspecies of MABC, comparable to those obtained by rpoB sequence typing. Nature Publishing Group UK 2020-10-02 /pmc/articles/PMC7532137/ /pubmed/33009494 http://dx.doi.org/10.1038/s41598-020-73607-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Minias, Alina Żukowska, Lidia Lach, Jakub Jagielski, Tomasz Strapagiel, Dominik Kim, Su-Young Koh, Won-Jung Adam, Heather Bittner, Ruth Truden, Sara Žolnir-Dovč, Manca Dziadek, Jarosław Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex |
title | Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex |
title_full | Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex |
title_fullStr | Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex |
title_full_unstemmed | Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex |
title_short | Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex |
title_sort | subspecies-specific sequence detection for differentiation of mycobacterium abscessus complex |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532137/ https://www.ncbi.nlm.nih.gov/pubmed/33009494 http://dx.doi.org/10.1038/s41598-020-73607-x |
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