A compendium of DNA-binding specificities of transcription factors in Pseudomonas syringae

Pseudomonas syringae is a Gram-negative and model pathogenic bacterium that causes plant diseases worldwide. Here, we set out to identify binding motifs for all 301 annotated transcription factors (TFs) of P. syringae using HT-SELEX. We successfully identify binding motifs for 100 TFs. We map functi...

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Detalles Bibliográficos
Autores principales: Fan, Ligang, Wang, Tingting, Hua, Canfeng, Sun, Wenju, Li, Xiaoyu, Grunwald, Lucas, Liu, Jingui, Wu, Nan, Shao, Xiaolong, Yin, Yimeng, Yan, Jian, Deng, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532196/
https://www.ncbi.nlm.nih.gov/pubmed/33009392
http://dx.doi.org/10.1038/s41467-020-18744-7
Descripción
Sumario:Pseudomonas syringae is a Gram-negative and model pathogenic bacterium that causes plant diseases worldwide. Here, we set out to identify binding motifs for all 301 annotated transcription factors (TFs) of P. syringae using HT-SELEX. We successfully identify binding motifs for 100 TFs. We map functional interactions between the TFs and their targets in virulence-associated pathways, and validate many of these interactions and functions using additional methods such as ChIP-seq, electrophoretic mobility shift assay (EMSA), RT-qPCR, and reporter assays. Our work identifies 25 virulence-associated master regulators, 14 of which had not been characterized as TFs before.

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