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A confocal Raman microscopic visualization of small penetrants in cellulose acetate using a deuterium-labeling technique
The purpose of the present study was to visualize the sorption dynamics of small compounds, such as propylene glycol (PG) in cellulose acetate (CA) film, by deuterium (d) labeling-aided confocal Raman spectroscopy (CRM). Substitution of hydrogen atoms in the target molecule with deuterium caused a m...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532217/ https://www.ncbi.nlm.nih.gov/pubmed/33009478 http://dx.doi.org/10.1038/s41598-020-73464-8 |
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author | Kubota, Hiroyuki Sakamoto, Koji Matsui, Toshiro |
author_facet | Kubota, Hiroyuki Sakamoto, Koji Matsui, Toshiro |
author_sort | Kubota, Hiroyuki |
collection | PubMed |
description | The purpose of the present study was to visualize the sorption dynamics of small compounds, such as propylene glycol (PG) in cellulose acetate (CA) film, by deuterium (d) labeling-aided confocal Raman spectroscopy (CRM). Substitution of hydrogen atoms in the target molecule with deuterium caused a marked shift of C-H bond-related Raman bands to low wavenumbers, while the number of deuterium did not affect the magnitude of the shift. Raman bands derived from the stretching vibration of C–H near 2900 cm(−1) for PG and ethanol were shifted to approximately 2100 cm(−1) for PG-d6 and ethanol-d5 in the silent region of the CA Raman spectrum. When PG-d6 was dissolved in glycerol triacetate (GTA), the observed Raman intensity ratio at 2123 cm(−1) of PG-d6 against 1739 cm(−1) of GTA (C=O bond-related) showed a linear relationship between the molar and intensity ratios, indicating that the observed Raman intensity can be used for quantitative assay of the target in polymer film. The depth profiling experiments by CRM revealed that the distribution (or sorption) of PG-d6 in CA film was successfully visualized as a function of Raman band at the characteristic 2123 cm(−1) intensity ratio. |
format | Online Article Text |
id | pubmed-7532217 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-75322172020-10-06 A confocal Raman microscopic visualization of small penetrants in cellulose acetate using a deuterium-labeling technique Kubota, Hiroyuki Sakamoto, Koji Matsui, Toshiro Sci Rep Article The purpose of the present study was to visualize the sorption dynamics of small compounds, such as propylene glycol (PG) in cellulose acetate (CA) film, by deuterium (d) labeling-aided confocal Raman spectroscopy (CRM). Substitution of hydrogen atoms in the target molecule with deuterium caused a marked shift of C-H bond-related Raman bands to low wavenumbers, while the number of deuterium did not affect the magnitude of the shift. Raman bands derived from the stretching vibration of C–H near 2900 cm(−1) for PG and ethanol were shifted to approximately 2100 cm(−1) for PG-d6 and ethanol-d5 in the silent region of the CA Raman spectrum. When PG-d6 was dissolved in glycerol triacetate (GTA), the observed Raman intensity ratio at 2123 cm(−1) of PG-d6 against 1739 cm(−1) of GTA (C=O bond-related) showed a linear relationship between the molar and intensity ratios, indicating that the observed Raman intensity can be used for quantitative assay of the target in polymer film. The depth profiling experiments by CRM revealed that the distribution (or sorption) of PG-d6 in CA film was successfully visualized as a function of Raman band at the characteristic 2123 cm(−1) intensity ratio. Nature Publishing Group UK 2020-10-02 /pmc/articles/PMC7532217/ /pubmed/33009478 http://dx.doi.org/10.1038/s41598-020-73464-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kubota, Hiroyuki Sakamoto, Koji Matsui, Toshiro A confocal Raman microscopic visualization of small penetrants in cellulose acetate using a deuterium-labeling technique |
title | A confocal Raman microscopic visualization of small penetrants in cellulose acetate using a deuterium-labeling technique |
title_full | A confocal Raman microscopic visualization of small penetrants in cellulose acetate using a deuterium-labeling technique |
title_fullStr | A confocal Raman microscopic visualization of small penetrants in cellulose acetate using a deuterium-labeling technique |
title_full_unstemmed | A confocal Raman microscopic visualization of small penetrants in cellulose acetate using a deuterium-labeling technique |
title_short | A confocal Raman microscopic visualization of small penetrants in cellulose acetate using a deuterium-labeling technique |
title_sort | confocal raman microscopic visualization of small penetrants in cellulose acetate using a deuterium-labeling technique |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532217/ https://www.ncbi.nlm.nih.gov/pubmed/33009478 http://dx.doi.org/10.1038/s41598-020-73464-8 |
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